Sarcolectin (SCL): Structure and expression of the recombinant molecule

Kaba, Aboubacar; Jiang, Pan; F, Chany-Fournier; Chany, C

doi:10.1016/s0300-9084(99)80128-x

Cited by 9 publications

(5 citation statements)

References 21 publications

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“…The type II cytokeratin is specifically expressed in the epithelial lining of the internal organ cavities, gland ducts, and blood vessels. In addition, the K2C7 monomer sarcolecti functions as a tissue growth factor in balance with interferons; thus, it may be involved in the regulation of cell growth and differentiation [40].…”

Section: Discussionmentioning

confidence: 99%

Proliferating cell nuclear antigen in the cytoplasm interacts with components of glycolysis and cancer

Naryzhny

Lee

2010

FEBS Letters

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MINT‐7995351: G3P (uniprotkb:P04406) and PCNA (uniprotkb:P12004) colocalize (MI:0403) by fluorescence microscopy (MI:0416)MINT‐7995334: ENOA (uniprotkb:P06733) and PCNA (uniprotkb:P12004) colocalize (MI:0403) by fluorescence microscopy (MI:0416)MINT‐7995368: ALDOA (uniprotkb:P04075) and PCNA (uniprotkb:P12004) colocalize (MI:0403) by fluorescence microscopy (MI:0416)MINT‐7995141: G3P (uniprotkb:P04406) binds (MI:0407) to PCNA (uniprotkb:P12004) by far western blotting (MI:0047)MINT‐7995182: ENOA (uniprotkb:P06733) binds (MI:0407) to PCNA (uniprotkb:P12004) by far western blotting (MI:0047)MINT‐7995132: G3P (uniprotkb:P04406) physically interacts (MI:0915) with PCNA (uniprotkb:P12004) by far western blotting (MI:0047)MINT‐7995228: PRDX6 (uniprotkb:P30041) physically interacts (MI:0915) with PCNA (uniprotkb:P12004) by far western blotting (MI:0047)MINT‐7995220: CAH2 (uniprotkb:P00918) physically interacts (MI:0915) with PCNA (uniprotkb:P12004) by far western blotting (MI:0047)MINT‐7995114: Triosephosphate isomerase (uniprotkb:P60174) binds (MI:0407) to PCNA (uniprotkb:P12004) by far western blotting (MI:0047)MINT‐7995244: K2C7 (uniprotkb:P08729) physically interacts (MI:0915) with PCNA (uniprotkb:P12004) by far western blotting (MI:0047)MINT‐7995252: ANXA2 (uniprotkb:P07355) physically interacts (MI:0915) with PCNA (uniprotkb:P12004) by far western blotting (MI:0047)MINT‐7995122: Triosephosphate isomerase (uniprotkb:P60174) physically interacts (MI:0915) with PCNA (uniprotkb:P12004) by far western blotting (MI:0047)MINT‐7995093: ALDOA (uniprotkb:P04075) physically interacts (MI:0915) with PCNA (uniprotkb:P12004) by far western blotting (MI:0047)MINT‐7995148: PGK1 (uniprotkb:P00558) physically interacts (MI:0915) with PCNA (uniprotkb:P12004) by far western blotting (MI:0047)MINT‐7995158: PGAM1 (uniprotkb:P18669) physically interacts (MI:0915) with PCNA (uniprotkb:P12004) by far western blotting (MI:0047)MINT‐7995166: PGAM1 (uniprotkb:P18669) binds (MI:0407) to PCNA (uniprotkb:P12004) by far western blotting (MI:0047)MINT‐7995105: ALDOA (uniprotkb:P04075) binds (MI:0407) to PCNA (uniprotkb:P12004) by far western blotting (MI:0047)MINT‐7995260: PPIA (uniprotkb:P62937) physically interacts (MI:0915) with PCNA (uniprotkb:P12004) by far western blotting (MI:0047)MINT‐7995173: ENOA (uniprotkb:P06733) physically interacts (MI:0915) with PCNA (uniprotkb:P12004) by far western blotting (MI:0047)MINT‐7995268: EF1A (uniprotkb:P68104) physically interacts (MI:0915) with PCNA (uniprotkb:P12004) by far western blotting (MI:0047)MINT‐7995236: MDHM (uniprotkb:P40926) physically interacts (MI:0915) with PCNA (uniprotkb:P12004) by far western blotting (MI:0047)MINT‐7995189: RSSA (uniprotkb:P08865) physically interacts (MI:0915) with PCNA (uniprotkb:P12004) by far western blotting (MI:0047)MINT‐7995282: PCNA (uniprotkb:P12004) physically interacts (MI:0915) with ALDOA (uniprotkb:P00883) and G3P (uniprotkb:P46406) by anti bait coimmunoprecipitation (MI:0006).

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Section: Discussionmentioning

confidence: 99%

Proliferating cell nuclear antigen in the cytoplasm interacts with components of glycolysis and cancer

Naryzhny

Lee

2010

FEBS Letters

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“…Results of immunocytochemical (ICC) staining and western blot showed that the level of cytokeratin 7 (CK7) expression increased after CoCl 2 treatment. CK7 was first isolated and purified by Kaba et al in 1988 8 and was also named sarcolectin (SCL) because of its high concentration in human osteosarcomas 9. Cytokeratin 7 ( CK7) is specifically expressed in the simple epithelia lining the cavities of internal organs and in transitional epithelium.…”

Section: Introductionmentioning

confidence: 99%

CK7 expression associates with the location, differentiation, lymph node metastasis, and the Dukes' stage of primary colorectal cancers

Fei

Cao³

et al. 2019

J. Cancer

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Purpose : Most colorectal cancers (CRCs) show positive immunohistochemical (IHC) staining for CK20 and negative staining for CK7. However, in clinical settings, some CRCs show positive IHC staining for CK7, and the clinicopathological significance of this needs to be studied. This study investigated the clinicopathological significance of CK7 positivity in CRCs. Materials and Methods : A total of 178 patients with CRC were used to study the clinicopathological significance of CK7 positivity. Western blotting and immunocytochemical (ICC) staining were used to compare the expression levels of CK7 before and after CoCl 2 treatment. Results : CK7 expression was associated with the location, differentiation, lymph node metastasis, and the Dukes' stage of CRCs. CK7 positive cells were mainly distributed at the edge of cancer nests, at the invasion front, as single stromal polyploid giant cancer cells (PGCCs), in tumor buds, in intravascular tumor emboli, and in a micropapillary pattern. Results of ICC staining showed that CK7 expression was almost negative in LoVo and HCT116 before CoCl 2 treatment. After CoCl 2 treatment, the PGCCs and their daughter cells of LoVo and HCT116 yielded positive results in CK7 ICC staining. Results of western blotting also confirmed that there was higher CK7 expression in LoVo and HCT116 after CoCl 2 treatment than in the control. Conclusion : CRC cells expressing CK7 may have strong invasive and metastatic abilities. Some metastasis-related morphological characteristics in CRCs including the invasion front, micropapillary pattern, tumor emboli, and single stromal PGCCs associated with CK7 positive expression.

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“…In some experiments, commercial K2C7 monoclonal antibodies (mAb) were used (Sigma C-6417) because of the close structural relationship between SCL and mesothelial cytokeratin K2C7 as control. (6) ELISA assay ELISA assay was performed by the routine procedure. The antigen was found to 96-well EIA/RIA flat-bottom plates (Costar, Cambridge, MA) by incubating the preparation overnight at 4°C or 2 h at ambient temperature.…”

Section: Polyclonal Monospecific Monoclonal and Oligopeptide Antibomentioning

confidence: 99%

“…Membranes were blocked 2 h with PBS containing 0.05% Tween 20 and 5% skim milk at ambient temperature and then incubated with primary antibodies overnight at 4°C (or 2 h at ambient temperature). The following antibodies were used: antibodies raised against NANApurified SCL antigens, oligopeptide antibodies synthesized according to the SCL sequences listed above, or commercial mAb K2C7 (Sigma C-6417) as reported (6) (all diluted 1:200). Bound antibody was probed with a second antibody combined with horseradish peroxidase (HRP) monkey antirabbit IgG (Amersham Pharmacia Biotech RPN 1064, Paris, France) at a final dilution of 1:20,000 in PBS-Tween for 1 h. Immunoreactive proteins were visualized using an enhanced chemoluminescence (ECL) detection kit (Amersham Pharmacia Biotech) according to the supplier's instructions.…”

Section: Immunoblotting Analysismentioning

confidence: 99%

See 1 more Smart Citation

The Interferon Antagonist Sarcolectin in the Progress of HIV-1 Infection and in AIDS

Kaba

Ilunga

Achour

et al. 2002

Journal of Interferon & Cytokine Research

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Sarcolectin (SCL) is a nonspecific stimulator of cellular DNA synthesis that was found in all animal sera tested to date. It inhibits the established interferon (IFN)-dependent antiviral state, restoring cells to their normal status. In this study, we examined the excretion/secretion of the IFN antagonist SCL in sera from healthy donors and in sera collected during different periods of human immunodeficiency virus type 1 (HIV-1) infection. We followed HIV-1-infected patients during all stages of development (seroconversion, initial and advanced phases of AIDS) and found a significant increase in SCL in sera of HIV-infected patients compared with seronegative subjects used as controls. This increase was established during seroconversion, and then the titers leveled off. In the final stage of the disease, the SCL titer increased again very significantly. We attribute this rapid rise to the virus-dependent destruction of T cells that can no longer be repaired. The high SCL level observed at this final stage, which is most predictive of the disease's progression, suggests that the action, rather than the production, of IFN is impaired.

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Sarcolectin (SCL): Structure and expression of the recombinant molecule

Cited by 9 publications

References 21 publications

Proliferating cell nuclear antigen in the cytoplasm interacts with components of glycolysis and cancer

Proliferating cell nuclear antigen in the cytoplasm interacts with components of glycolysis and cancer

CK7 expression associates with the location, differentiation, lymph node metastasis, and the Dukes' stage of primary colorectal cancers

The Interferon Antagonist Sarcolectin in the Progress of HIV-1 Infection and in AIDS

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