Sarcoplasmic Calcium-Binding Protein: Identification as a New Allergen of the Black Tiger Shrimp <i>Penaeus monodon</i>

Abstract

Background: Tropomyosin and arginine kinase have been identified as crustacean allergens. During purification of arginine kinase from black tiger shrimp Penaeus monodon, we found a new allergen of 20-kDa. Methods: A 20-kDa allergen was purified from the abdominal muscle of black tiger shrimp by salting-out, anion-exchange HPLC and reverse-phase HPLC. Following digestion of the 20-kDa allergen with lysyl endopeptidase, peptide fragments were isolated by reverse-phase HPLC, and 2 of them were sequenced. The 20-kDa allergen, together with tropomyosin and arginine kinase purified from black tiger shrimp, was evaluated for IgE reactivity by ELISA. Five species of crustaceans (kuruma shrimp, American lobster, pink shrimp, king crab and snow crab) were surveyed for the 20-kDa allergen by immunoblotting. Results: The 20-kDa allergen was purified from black tiger shrimp and identified as a sarcoplasmic calcium-binding protein (SCP) based on the determined amino acid sequences of 2 enzymatic fragments. Of 16 sera from crustacean-allergic patients, 8 and 13 reacted to SCP and tropomyosin, respectively; the reactivity to arginine kinase was weakly recognized with 10 sera. In immunoblotting, an IgE-reactive 20-kDa protein was also detected in kuruma shrimp, American lobster and pink shrimp but not in 2 species of crab. Preadsorption of the sera with black tiger shrimp SCP abolished the IgE reactivity of the 20-kDa protein, suggesting the 20-kDa protein to be an SCP. Conclusions: SCP is a new crustacean allergen, and distribution of IgE-reactive SCP is probably limited to shrimp and crayfish.

Shrimp Allergy in Italian Adults: A Multicenter Study Showing a High Prevalence of Sensitivity to Novel High Molecular Weight Allergens

“…Recently, several new shrimp allergens have been detected, including arginine kinase (Pen m 2, Lit v 2; 40 kDa) [9,10], sarcoplasmic calcium-binding protein (Lit v 4; 20 kDa) [11,12] and myosin light chain (Lit v 3; 20 kDa) [13]. By comparing the molecular weight of these allergens with those recognized by our patients on immunoblot analyses, it is possible to conclude that a proportion of our Pen a 1-negative patients was most probably reactive to AK, SCB or MLC.…”

“…Tropomyosin has been considered the major allergen in shrimp and molluscs and a minor allergen in other invertebrates ever since, and it has been reported that 85% of shrimp-allergic individuals show reactivity to this protein [8]. In recent years, several other shrimp allergens have been detected and characterized, including arginine kinase [9,10], sarcoplasmic calcium-binding protein [11,12] and myosin light chain [13], although little is known about the clinical relevance of these allergen proteins, and large population-based studies of shrimp allergy are missing. In the present study many Italian allergy centers participated in the detection and clinical characterization of shrimp-allergic subjects with the aim to investigate which are the shrimp allergens most frequently associated with clinically relevant shrimp allergy in this country.…”

Extraction of Squid (Photololigo Duvaucelii) Myofibrillar and Sarcoplasmic Proteins to Create A Skin Prick Test Reagent In The Diagnosis of Food Allergy

“…Sarcoplasmic protein (A) SDS-PAGE Electrophoresis results of squid sarcoplasmic protein Several seafood sarcoplasmic proteins which can cause allergens is the sarcoplasmic calcium binding protein with 20 kDa molecular weight [20], hemocyanin with 72 kDa molecular weight [21], arginine kinase with 42 kDa molecular weight [22] and amylase [23]. Protein with a molecular weight of 42 kDa is suspected as arginine kinase that can bind to specific IgE antibodies in 4, 5 and 12 serums.…”

El camarón como una fuente de alérgenos

“…monodon, la arginina-cinasa (Pen m2) es capaz de unir anticuerpos IgE de 62 % de las personas alérgicas (42). Este alérgeno presenta 92 % de identidad en la secuencia de aminoácidos con Lit v 2 y Cra c 2 del camarón Crangon crangon, hecho que podría tener relevancia en la reacción cruzada entre estas especies (43)(44)(45).…”

“…Este alérgeno se identificó en las especies de camarón L. vannamei, P. monodon y C. cangron, y en la nomenclatura oficial corresponde al grupo 4 (42,43,51 …”