SARI , a novel target gene of glucocorticoid receptor, plays an important role in dexamethasone-mediated killing of B lymphoma cells

Huang, Yinghui; Zhou, Jie; Huang, Yan; He, Jintao; Wang, Yuting; Yang, Chao‐Hui; Liu, Dongbo; Zhang, Li; He, Fengtian

doi:10.1016/j.canlet.2016.01.034

Cited by 24 publications

(41 citation statements)

References 40 publications

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“…In agreement with previous evidence for the tumour suppressor role of SARI in mouse models of colon and other cancers, 15,19,20 we showed that SARI deficiency enhances intestinal tumorigenesis in an inflammation-dependent model. AOM is a chemical agent that initiates cancer via facilitating base mispairings, 21 whereas DSS is a pro-inflammatory reagent for mimicking chronic colitis-associated tumour development when combined with AOM.…”

Section: Discussionsupporting

confidence: 92%

SARI suppresses colitis‐associated cancer development by maintaining MCP‐1‐mediated tumour‐associated macrophage recruitment

Dai

Liu

Yin

et al. 2019

J Cellular Molecular Medi

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SARI (suppressor of AP‐1, regulated by IFN) impaired tumour growth by promoting apoptosis and inhibiting cell proliferation and tumour angiogenesis in various cancers. However, the role of SARI in regulating tumour‐associated inflammation microenvironment is still elusive. In our study, the colitis‐dependent and ‐independent primary model were established in SARI deficiency mice and immuno‐reconstructive mice to investigate the functional role of SARI in regulating tumour‐associated inflammation microenvironment and primary colon cancer formation. The results have shown that SARI deficiency promotes colitis‐associated cancer (CAC) development only in the presence of colon inflammation. SARI inhibited tumour‐associated macrophages (TAM) infiltration in colon tissues, and SARI deficiency in bone marrow cells has no observed role in the promotion of intestinal tumorigenesis. Mechanism investigations indicated that SARI down‐regulates p‐STAT1 and STAT1 expression in colon cancer cells, following inhibition of MCP‐1/CCR2 axis activation during CAC development. Inverse correlations between SARI expression and macrophage infiltration, MCP‐1 expression and p‐STAT1 expression were also demonstrated in colon malignant tissues. Collectively, our results prove the inhibition role of SARI in colon cancer formation through regulating TAM infiltration.

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Section: Discussionsupporting

confidence: 92%

SARI suppresses colitis‐associated cancer development by maintaining MCP‐1‐mediated tumour‐associated macrophage recruitment

Dai

Liu

Yin

et al. 2019

J Cellular Molecular Medi

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“…ChIP assays were performed using a ChIP kit (Millipore, Billerica, MA) as described [34]. Briefly, HepG2 cells were treated with PA (0.2 mM) or vehicle control (0.1% ethanol) for 24 h, and then fixed with 1% formaldehyde for 10 min at 37 °C to cross-link the nuclear proteins to DNA.…”

Section: Chromatin Immunoprecipitation (Chip) Analysismentioning

confidence: 99%

Resveratrol Ameliorates Lipid Droplet Accumulation in Liver Through a SIRT1/ ATF6-Dependent Mechanism

Zhou

et al. 2018

Cell Physiol Biochem

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Background/Aims: Lipid droplets (LDs) are dynamic organelles that store neutral lipids during times of energy excess, and an increased accumulation of LDs in the liver is closely linked to hepatic steatosis. Our previous studies suggested that resveratrol (RSV) supplement could improve hepatic steatosis, but the underlying mechanism, particularly which related to LD accumulation, has not yet been elucidated. Methods: A high-fat diet (HFD) and palmitic acid were used to induce hepatic steatosis in mouse liver and hepatocytes, respectively. The effects of RSV on LD accumulation were analyzed in vivo and in vitro. The effects of RSV on the expression levels of LD-associated genes (ATF6, Fsp27β/CIDEC, CREBH, and PLIN1) were measured by qRT-PCR and western blot assays, followed by KD or overexpression of SIRT1 and ATF6 with small interfering RNAs or overexpressed plasmids, respectively. The dual luciferase reporter assay, chromatin immunoprecipitation assay, coimmunoprecipitation, and proximity ligation assay were utilized to clarify the mechanism of transcriptional regulation and possible interaction between SIRT1 and ATF6. Results: There was a significant increase in the accumulation of LDs in liver and hepatocytes during the process of HFD-induced steatosis, respectively, which was significantly inhibited by RSV supplementation. RSV notably activated SIRT1 expression and decreased the expression levels of ATF6, Fsp27β/CIDEC, CREBH, and PLIN1, which are associated with LD accumulation. Interestingly, the inhibitory effects of RSV on LD accumulation and the associated expression of genes in hepatocytes were abrogated or strengthened with SIRT1 silencing or overexpression, respectively. On the contrary, the benefits of RSV in hepatocytes were eliminated or aggravated when transfected with the overexpressed ATF6 or ATF6 siRNA, respectively. Furthermore, we found that RSV stimulated SIRT1 expression significantly, which was followed by increased deacetylation and inactivation of ATF6, resulting in a positive feedback loop for SIRT1 transcription associated with ATF6 binding to the SIRT1 promoter region. Conclusion: Taken together, these findings indicate that RSV supplementation improves hepatic steatosis by ameliorating the accumulation of LDs, and this might be partially mediated by a SIRT1/ATF6-dependent mechanism.

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“…Cell proliferation was measured using Cell Counting Kit-8 (Dojindo Laboratories, Kumamoto, Japan) as previously described 38. HT-29 and HCT116 cells were treated with vehicle control or various doses of IR-58 for 48 hours, with or without 3MA.…”

Section: Methodsmentioning

confidence: 99%

“…Real-time PCR was performed using a SYBR Green qPCR master mix (Takara) according to the manufacturer's protocol, as previously described 38. The primers for the qRT-PCR are listed in online supplementary table S1.…”

Section: Methodsmentioning

confidence: 99%

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Identification of a fluorescent small-molecule enhancer for therapeutic autophagy in colorectal cancer by targeting mitochondrial protein translocase TIM44

Huang

Zhou

Luo

et al. 2016

Gut

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SARI , a novel target gene of glucocorticoid receptor, plays an important role in dexamethasone-mediated killing of B lymphoma cells

Cited by 24 publications

References 40 publications

SARI suppresses colitis‐associated cancer development by maintaining MCP‐1‐mediated tumour‐associated macrophage recruitment

SARI suppresses colitis‐associated cancer development by maintaining MCP‐1‐mediated tumour‐associated macrophage recruitment

Resveratrol Ameliorates Lipid Droplet Accumulation in Liver Through a SIRT1/ ATF6-Dependent Mechanism

Identification of a fluorescent small-molecule enhancer for therapeutic autophagy in colorectal cancer by targeting mitochondrial protein translocase TIM44

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