SARS-CoV-2 Inactivation by Ozonated Water: A Preliminary Alternative for Environmental Disinfection

Martins, Ronaldo B.; Castro, Ítalo A.; Pontelli, Marjorie Cornejo; Souza, Juliano P.; Lima, Thais M.; Melo, Stella Rezende; Siqueira, João Paulo Zen; Caetano, Maicon Henrique; Arruda, Eurico; Almeida, Margarete Teresa Gottardo de

doi:10.1080/01919512.2020.1842998

Cited by 37 publications

(28 citation statements)

References 12 publications

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“…to inactivate SARS-CoV-2 on different materials, most of them require long duration and energy consumption and even present side effects on the human body and environment. 33–36 Thus, a more effective, economical, without side effects, and green mean to avoid environmental transmission of SARS-CoV-2 is urgent.…”

Section: Introductionmentioning

confidence: 99%

Cold atmospheric plasma: A non-negligible strategy for viral RNA inactivation to prevent SARS-CoV-2 environmental transmission

Jin

Dai

et al. 2021

AIP Advances

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Cold atmospheric plasma (CAP), regarded as a powerful physics technology, displays antimicrobial, antitumor, and even antiviral properties, but the underlying mechanism is rarely studied. In this study, four CAP exposure doses (30, 60, 120, and 240 s) were applied to inactivate a severe acute respiratory syndrome coronavirus 2 like pseudovirus on a stainless steel disk, which comprised spike protein on its membrane and can express a green fluorescent protein. In order to unravel the potential effects of CAP irradiation on pseudovirus, infection assay, optical emission spectra analysis, transmission electron microscopy (TEM), sodium dodecyl sulfate polyacrylamide gel electrophoresis, ELISA, and qPCR experiments were carried out. As a result, our study indicated that CAP irradiation can significantly decrease the infectivity of pseudovirus in a dose dependent manner through destroying the cell membrane and further damaging viral RNA, with the molecular weight and conformation of spike receptor binding domain protein unchanged.

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Section: Introductionmentioning

confidence: 99%

Cold atmospheric plasma: A non-negligible strategy for viral RNA inactivation to prevent SARS-CoV-2 environmental transmission

Jin

Dai

et al. 2021

AIP Advances

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“…OW has recently been shown to reduce the infectivity of SARS-CoV-2 following exposure of the virus to low concentrations of OW for 1 min (Martins et al, 2021 ). In addition, 10 ppm OW, which is a high ozone concentration for OW, rapidly inactivates SARS-CoV-2 (Inagaki et al, 2020).…”

Section: Discussionmentioning

confidence: 99%

“…In particular, OW is known as a powerful inactivating agent against bacteria, fungi, protozoa, and viruses (Kim et al, 1999 ), and has been suggested to exert antimicrobial effects in running water (Nakamura et al, 2018 ). More recently, low concentrations of OW were shown to reduce viral infectivity upon exposure to SARS-CoV-2 for 1 min (Martins et al, 2021 ). In addition, it was reported that high ozone concentrations in OW rapidly inactivated SARS-CoV-2 (Inagaki et al, 2021 ).…”

Section: Introductionmentioning

confidence: 99%

Inactivation of SARS-CoV-2 by Ozonated Glycerol

et al. 2021

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We evaluated the SARS-CoV-2-inactivation activity of ozonated glycerol (OG). When a viral solution with 1% fetal bovine serum (FBS) was mixed with test solutions at a ratio of 1:19 and incubated for 20 s, OG with ozone concentrations of over 1000 ppm inactivated ≥ 94.38% of the virus. Extension of the reaction time to 1 h led to the inactivation of ≥ 99.82% of the virus (the viral titer was below the detection limit). Extension to 24 h resulted in concentrations over 200 ppm OG inactivating ≥ 99.87% of the virus (the viral titers were below the detection limit). Next, viral solutions with 1, 20, and 40% FBS were mixed with test solutions at a ratio of 1:19 and incubated for 5 min. Whereas the virucidal activity of 500 ppm OG was very limited in the presence of 1% FBS (79.47% inactivation), it increased in the presence of 20 and 40% FBS (95.13 and 97.95% inactivation, respectively; the viral titers were not below the detection limit). Meanwhile, over 1000 ppm OG inactivated ≥ 99.44% of the virus regardless of the FBS concentration (the viral titers were below the detection limit). Extension of the reaction time to 1 h led to 500 ppm OG inactivating ≥ 99.91 and ≥ 99.95% of the virus with 20 and 40% FBS, respectively (the viral titers were below the detection limit). These results suggested that OG might be useful as a virucidal agent against SARS-CoV-2.

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“…To quantify the amount of virus produced, a tenfold serial dilution of the plasmid was prepared in the range from 10 6 to 1 plasmid copy. The coefficient of determination ( R 2 ) for the plasmid standard curve was 0.999, with efficiency above 91% reached using any set of primers/probe 51 .…”

Section: Models and Methodsmentioning

confidence: 97%

Drug repurposing to face Covid-19: Celastrol, a potential leading drug capable of inhibiting SARS-CoV-2 replication and induced inflammation

Fuzo

Martins

Fraga-Silva

et al. 2021

Preprint

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The global emergence of Covid-19 has caused huge human casualties. Clinical manifestations of the disease vary from asymptomatic to lethal, and the symptomatic form can be associated with cytokine storm and non-homeostatic inflammatory response. In face of the urgent demand for effective drugs to treat Covid-19, we have searched for candidate compounds using a drug repurposing approach based on in silico analysis followed by biological validation. Here we identified celastrol, a pentacyclic triterpene isolated from Tripterygium wilfordii Hook F – a plant used in traditional Chinese medicine – as one of the best compounds out of 39 repurposed drug candidates. Celastrol reverted gene expression signature from SARS-CoV-2-infected cells; bound with high-affinity energy to viral molecular targets such as main protease (Mpro) and receptor-biding domain (RBD); inhibited SARS-CoV-2 replication in monkey (Vero and Vero-ACE2) and human (Caco-2 and Calu-3) cell lines; and decreased interleukin-6 (IL-6) secretion in SARS-CoV-2-infected human cell lines. Interestingly, celastrol acted in a concentration-dependent manner, with undetectable signs of cytotoxicity. Therefore, celastrol is a promising lead drug candidate to treat Covid-19 due to its ability to suppress SARS-CoV-2 replication and IL-6 production in infected cells, two critical events in the pathophysiology of this disease.

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SARS-CoV-2 Inactivation by Ozonated Water: A Preliminary Alternative for Environmental Disinfection

Cited by 37 publications

References 12 publications

Cold atmospheric plasma: A non-negligible strategy for viral RNA inactivation to prevent SARS-CoV-2 environmental transmission

Cold atmospheric plasma: A non-negligible strategy for viral RNA inactivation to prevent SARS-CoV-2 environmental transmission

Inactivation of SARS-CoV-2 by Ozonated Glycerol

Drug repurposing to face Covid-19: Celastrol, a potential leading drug capable of inhibiting SARS-CoV-2 replication and induced inflammation

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