SARS, SARS again, and MERS. Review of animal models of human respiratory syndromes caused by coronavirus infections

Nagornykh, Aleksey M.; Tyumentsev, Aleksandr I.; Tyumentseva, Marina A.; Акимкин, В. Г.

doi:10.36233/0372-9311-2020-97-5-6

Cited by 4 publications

(2 citation statements)

References 53 publications

(128 reference statements)

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“…Сфероидтардың қой шешегі вирусын жұқтырғаннан кейін бірінші пассажда вирус титрі 5,33±0,04 lg ТЦД 50 /см 3 болды. Екінші және үшінші пассажда вирустың биологиялық белсенділігі сәйкесінше 6,33±0,04 және 6,41±0,04 lg ТЦД 50 /см 3…”

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Culture of sheep poxvirus in a 3D Vero cells culture model

Nakhanov,

Zhapparova,

Bulatov

et al. 2024

BMG

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In the article the production of a three-dimensional culture of Vero cells using the methods of “hanging drop” and roller cultivation was described. The resulting spheroids remain viable for more than four days. For the formation of large spheroids with good proliferative activity, the inoculum concentration should be 200000 cells/ml. The additional study of the cell proliferation index in spheroids showed that cell growth was 3.5 times on the second day. A comparative study of the biological activity of the sheep poxvirus in 3D cell culture was carried out in comparison with traditional2D cultivation. After infection of the spheroids with sheep poxvirus in the first passage, the virus titer was 5.33±0.04 lg TCID50/ml. At the second and third passages, the biological activity of the virus increased to 6.33±0.04 and 6.41±0.04 lg TCID50/ml, respectively. Considering these data, three-dimensional culture allows for obtaining much cell biomass with high biological activity compared to the traditional culture method.

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Section: Fundingunclassified

“…Viral infections are a significant global concern, with notable outbreaks caused by coronaviruses such as SARS-CoV, MERS-CoV, and SARS-CoV-2 [1][2][3]. Virology research has successfully utilized cell cultures as a crucial tool for researchers in various fields.…”

Section: Introductionmentioning

confidence: 99%

Culture of sheep poxvirus in a 3D Vero cells culture model

Nakhanov,

Zhapparova,

Bulatov

et al. 2024

BMG

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Features of the course of COVID-19 in patients with comorbid pathology

et al. 2022

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Comorbidity is an important problem of modern medicine, the study of which is a priority for public health. According to the literature, the prevalence of comorbidity reaches a third of the population in some countries, while there is a tendency for an increase in the number of such patients in all age groups. In the context of the COVID-19 pandemic, the presence of comorbidity in patients is a significant risk factor affecting the course and prognosis of a new coronavirus infection; at the same time, it is comorbid patients who belong to the most vulnerable group. The review presents data indicating a significant impact of comorbid pathologies on an increase in the number of complications and mortality; the most common combinations of diseases in patients with a new coronavirus infection were analyzed. There is an increase in the prevalence of comorbidity in all age groups, which dictates the need to develop an integrated interdisciplinary approach for such patients. The vast majority of clinical guidelines for the treatment of comorbidities in COVID-19 are mainly focused on individual diseases, which limits the use of these algorithms in comorbid patients. Due to the high risk of contracting a viral infection, as well as the frequent development of complications and mortality in comorbid patients, preventive measures should be focused simultaneously on two tasks: measures aimed at preventing infection with COVID-19 and compensating for comorbid pathology. In the case of SARS-CoV-2 infection, careful monitoring of such patients, most often in a hospital setting, is necessary in order to prevent complications and adverse outcomes.

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SARS-CоV-2 spread in humans and animals

Акимова

Семакина

Митрофанова

et al. 2021

Veterinariâ segodnâ

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Coronaviruses are a large family of viruses and they are wide spread in animals and humans. They can cause respiratory tract illnesses of various severity. The latest recently discovered coronavirus (SARS-CoV-2) is an agent ofCOVID-19. The first human cases were reported in Wuhan (People’s Republic of China) in December2019. Since then, the disease infected over 153 million people and became the cause of more than 3 million deaths all over the world. Among the leaders in the confirmed cases are the USA, India, Brazil, France, Turkey and Russia. In February 2020, it was determined that some animal species, including domestic cats and dogs, can be infected with SARS-CоV-2. Reports of animal infection in zoos were submitted from the USA, Argentina, Czech Republic, Sweden, Spain, Estonia, RSA and India. Cases of SARS-CoV-2 infection in fur-farmed minks were reported by 13countries. The most large-scale COVID-19 outbreak in minks that involved about 300 mink farms was reported in Denmark. During the COVID-19 pandemic, the agent’s transmission from humans to canines (Canidae), felines (Felidae), mustelids (Mustelidae) and hominids (Hominidae) was confirmed. As of early May2021, the disease cases in animals were reported by 33countries. Due to COVID-19 epidemic spread and detection of animal infection cases, diagnosis tools and methods were developed in the Russian Federation, and screening tests were performed in susceptible animal populations in different regions of the country. COVID-19 monitoring results demonstrated the virus in two cats (in Moscow and Tyumen).

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SARS, SARS again, and MERS. Review of animal models of human respiratory syndromes caused by coronavirus infections

Cited by 4 publications

References 53 publications

Culture of sheep poxvirus in a 3D Vero cells culture model

Culture of sheep poxvirus in a 3D Vero cells culture model

Features of the course of COVID-19 in patients with comorbid pathology

SARS-CоV-2 spread in humans and animals

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