Satellite DNA methylation status and expression of selected genes inBos indicusblastocysts producedin vivoandin vitro

Urrego, Rodrigo; Bernal-Ulloa, Sandra; Chavarría, N.; Herrera-Puerta, E.; Lucas‐Hahn, Andrea; Herrmann, Doris; Winkler, Sylke; Dorit, Pache; Niemann, Heiner; Rodríguez-Osorio, Nélida

doi:10.1017/s096719941600040x

Cited by 16 publications

(12 citation statements)

References 55 publications

(75 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Dramatic biological changes during embryonic development, such as gene expression with major embryonic genome activation [1] and epigenetic modifications with DNA methylation and histone modifications [2], have been analyzed in mammals, including cows. In bovine embryos, the methylation status and expression of specific genes in in vitro -developed blastocysts have been studied but suggested to be different from those in in vivo -derived blastocysts [3]. Observing in vivo -derived embryos is required to understand the precise mechanisms of biological changes during early embryonic development.…”

mentioning

confidence: 99%

The effects of electroporation on viability and quality of in vivo-derived bovine blastocysts

Tanihara

Hirata

Morikawa

et al. 2019

Journal of Reproduction and Development

View full text Add to dashboard Cite

The introduction of exogenous molecules into embryos is required for analyses of molecular dynamics and specific gene functions during early embryonic development. Electroporation is an effective method to transport exogenous molecules into cells, but is rarely used in bovine embryos. First, we evaluated the viability of in vivo-derived bovine blastocysts after electroporation with fluorescein (FAM) labeled-oligonucleotides with varying pulse numbers (3, 5, 7, and 10), while keeping the pulse duration at 1 msec and the electric field of 20 V/mm. Next, we examined the effects of zona pellucida status on blastocyst quality after electroporation, by comparing the average diameter of blastocysts before and after electroporation using blastocysts with intact zona pellucida and hatching/hatched blastocysts. Electroporation successfully introduced exogenous molecules into in vivo-derived bovine blastocysts without loss of viability. Moreover, the status of the zona pellucida may be associated with the quality of blastocysts after electroporation.

show abstract

mentioning

confidence: 99%

The effects of electroporation on viability and quality of in vivo-derived bovine blastocysts

Tanihara

Hirata

Morikawa

et al. 2019

Journal of Reproduction and Development

View full text Add to dashboard Cite

show abstract

“…The whole collection of satDNAs makes up large portions (usually >30%) of animal and plant genomes (reviewed by Plohl et al (2014) ). Although satDNAs do not code for proteins, they may play important cellular roles, including participation in chromatin packaging ( Blattes et al 2006 ; Feliciello et al 2015 ), centromere formation/maintenance ( Rošić et al 2014 ; Aldrup-MacDonald et al 2016 ), and gene regulation ( Menon et al 2014 ; Feliciello et al 2015 ; Urrego et al 2017 ).…”

mentioning

confidence: 99%

Dissecting the Satellite DNA Landscape in Three CactophilicDrosophilaSequenced Genomes

Svartman

Kuhn

2017

G3 Genes|Genomes|Genetics

View full text Add to dashboard Cite

Eukaryote genomes are replete with repetitive DNAs. This class includes tandemly repeated satellite DNAs (satDNA) which are among the most abundant, fast evolving (yet poorly studied) genomic components. Here, we used high-throughput sequencing data from three cactophilic Drosophila species, D. buzzatii, D. seriema, and D. mojavensis, to access and study their whole satDNA landscape. In total, the RepeatExplorer software identified five satDNAs, three previously described (pBuM, DBC-150 and CDSTR198) and two novel ones (CDSTR138 and CDSTR130). Only pBuM is shared among all three species. The satDNA repeat length falls within only two classes, between 130 and 200 bp or between 340 and 390 bp. FISH on metaphase and polytene chromosomes revealed the presence of satDNA arrays in at least one of the following genomic compartments: centromeric, telomeric, subtelomeric, or dispersed along euchromatin. The chromosomal distribution ranges from a single chromosome to almost all chromosomes of the complement. Fiber-FISH and sequence analysis of contigs revealed interspersion between pBuM and CDSTR130 in the microchromosomes of D. mojavensis. Phylogenetic analyses showed that the pBuM satDNA underwent concerted evolution at both interspecific and intraspecific levels. Based on RNA-seq data, we found transcription activity for pBuM (in D. mojavensis) and CDSTR198 (in D. buzzatii) in all five analyzed developmental stages, most notably in pupae and adult males. Our data revealed that cactophilic Drosophila present the lowest amount of satDNAs (1.9–2.9%) within the Drosophila genus reported so far. We discuss how our findings on the satDNA location, abundance, organization, and transcription activity may be related to functional aspects.

show abstract

“…La morfología de los COC se ha utilizado como un parámetro de selección de calidad de los oocitos ya que parece existir una relación entre la morfología y el desarrollo competente 27 . Algunos estudios realizados en B. taurus, han mostrado que COC con signos de atresia temprana determinada por su aspecto morfológico (leve expansión de las células del cúmulo periféricas y ooplasma granulado) parecen tener la misma o mejor capacidad de desarrollo que los considerados morfológicamente normales 13,18,28 , aunque actualmente el dogma y el proceder durante los procedimientos de PIVE consiste en incluir solamente los oocitos con cúmulo compacto y ooplasma homogeneo denominados como morfológicamente normales [29][30][31] dejando atrás posiblemente COC competentes que si fueran incluidos podrían aumentar el número de COC que entran a los programas de PIVE y por ende las posibilidades de mejorar las tasas de producción de blastocistos de dichos programas. El presente estudio permitió evaluar el efecto de la morfología de los COC con signos de atresia temprana sobre las tasas de desarrollo embrionario in vitro en bovinos B. indicus.…”

Section: Discussionunclassified

Producción in vitro de embriones a partir de complejos cúmulos oocitos tipo II en bovinos Bos indicus

Velez¹,

Chica²,

Urrego³

et al. 2017

Ces. Med. Vet. Zootec.

View full text Add to dashboard Cite

The morphological selection of cumulus-oocyte complexes (COCs) is an important step for in vitro embryo production. It has been suggested that COC showing signs of atresia have the ability to generate embryos. The objective of this work was to evaluate the effect of COC morphology from Bos indicus animals with signs of early atresia versus no signs of atresia on in vitro embryo production. COC were classified in: Type I (TI): homogeneous ooplasm with ≥ 4 layers of compact cumulus cells (CC) and Type II (TII): granular ooplasm and ≥ 4 layers of CC slightly expanded. The COC were matured in vitro for 24 hours in TCM199 medium and subsequently fertilized in vitro for 18 h. The suspected zygotes were cultured in vitro for seven days in modified SOFaa medium. Embryonic quality was determined by blastomeric count following staining with Hoechst 33342. Student test was used to determine statistical differences for cleavage, blastocyst rate and blastomeric counts between types of COC. The cleavage rate for TI (n = 220) and TII (n = 161) was 88 ± 4 % and 89 ± 8 % respectively (p> 0.05); embryo development rate was 36 ± 7 % and 33 ± 8 % (p>0.05) respectively. The blastomeric count for both groups was 101 and 104 cells for TI and TII respectively (n = 10), (p>0.05). These results demonstrate that there is no difference in the quantity and quality of embryos produced in vitro using COC type I or type II, suggesting that both types could be used for bovine in vitro embryo production in Bos indicus cows. Keywords: atresia, competence, cumulus-oocyte complex.Resumen La selección morfológica de los complejos cúmulo-oocito (COC) es crucial para la producción de embriones in vitro. Se ha sugerido que COC que muestran signos de atresia poseen capacidad de generar embriones. El objetivo de este trabajo fue evaluar el efecto de la morfología de los COC provenientes de animales Bos indicus con signos de atresia temprana y sin signos de atresia sobre la producción de embriones in vitro. Se clasificaron COC obtenidos de ovarios de faenado en dos grupos: Tipo I (TI): ooplasma homogéneo con ≥ 4 capas de células del cumulo (CC) compactas y Tipo II (TII): ooplasma

show abstract

Satellite DNA methylation status and expression of selected genes inBos indicusblastocysts producedin vivoandin vitro

Cited by 16 publications

References 55 publications

The effects of electroporation on viability and quality of <i>in vivo</i>-derived bovine blastocysts

The effects of electroporation on viability and quality of <i>in vivo</i>-derived bovine blastocysts

Dissecting the Satellite DNA Landscape in Three CactophilicDrosophilaSequenced Genomes

Producción in vitro de embriones a partir de complejos cúmulos oocitos tipo II en bovinos Bos indicus

Contact Info

Product

Resources

About