Satellite DNA sequence content of polylysine-titratable and nuclease-resistant fractions of mouse liver hepatoma chromatin

Duerksen, Jacob D.; Paul, Izhak J.

doi:10.1093/nar/3.9.2277

Cited by 6 publications

(2 citation statements)

References 35 publications

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“…We have illustrated the present technique by using mouse satellite DNA as a hybridization probe. Although this highly repetitive DNA sequence family has been shown previously to be packaged into nucleosomes (43)(44)(45), little was known with regard to the properties of the nucleosomes along this sequence. Our results show that satellite chromatin is relatively resistant to micrococcal nuclease digestion (Figs.…”

Section: Discussionmentioning

confidence: 99%

Transferring DNA from electrophoretically resolved nucleosomes to diazobenzyloxymethyl cellulose: properties of nucleosomes along mouse satellite DNA

Reudelhuber¹,

Ball²,

Davis³

et al. 1982

Nucl Acids Res

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Electrophoresis fractionates nucleosomes which possess different protein compositions. We report here a procedure for transferring the DNA components of electrophoretically resolved nucleosomes to diazobenzyloxymethyl cellulose (DBM-paper). Histones are first removed from nucleosome components by electrophoresis in the presence of cetyltrimethylammonium bromide (CTAB), leaving DNA fragments fixed within the original gel as the CTAB salts. The DNA is then converted to the sodium salt, denatured, and electrophoretically transferred to DBM-paper. The overall pattern of DNA on the resulting blot is visualized either by fluorography or by immunoautoradiography. This DNA pattern is then compared with autoradiograms obtained after hybridizing the same blot with specific 32P-labeled probes. Using mouse satellite DNA as a hybridization probe, we illustrate the above techniques and demonstrate that nucleosomes carrying satellite sequences are compositionally heterogeneous. The procedures described here should also be useful in the analysis of the nucleic acid components associated with other nucleoprotein complexes.

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Section: Discussionmentioning

confidence: 99%

Transferring DNA from electrophoretically resolved nucleosomes to diazobenzyloxymethyl cellulose: properties of nucleosomes along mouse satellite DNA

Reudelhuber¹,

Ball²,

Davis³

et al. 1982

Nucl Acids Res

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“…To this end, two different in vitro methods were employed: a murine hepatoma cell line, namely TLT cells, and precision-cut rat liver slices (PCLS). The first model (TLT cells) has been widely used to assess both in vivo and in vitro cytotoxic anticancer effects [12][13][14], facilitating the investigation of possible mechanisms involved in cancer cell death. The second method, PCLS, is a suitable model for studying the toxic effects of xenobiotics on healthy tissues at both molecular and cell biology levels [15][16][17][18].…”

Section: Introductionmentioning

confidence: 99%

An in vitro comparative study with furyl-1,4-quinones endowed with anticancer activities

et al. 2010

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We describe the biological activity of some furylbenzo- and naphthoquinones (furylquinones) on hepatocarcinoma cells and healthy rat liver slices. The effects of furylquinones on cancer cells (Transplantable Liver Tumor, TLT) were assessed by measuring cell death (membrane cell lysis); intracellular contents of ATP and GSH and the activity of caspase-3 were used to determine the type of cell death. Most of the furylquinones tested (at a concentration of 25 μg/ml) induced caspase-independent cell death but compound 4 had no cytotoxic effects. The levels of both ATP and GSH were severely affected by quinones 1, 2 and 5, while no effect was observed with compound 4. These cytotoxic properties of quinones are associated with physico-chemical properties as shown by the LUMO energies and lipophilicity. Interestingly, no cytotoxic effects of furylquinones were detected when the in vitro model of precision-cut liver slices (PCLS) was used. Indeed, although CYP2E1 activity was slightly affected, ATP and GSH levels as well as protein synthesis were not modified by furylquinones. Paracetamol, a well-known hepatotoxicant, reduced these parameters by more than 80% compared to control conditions. Taking into account the considerable incidence of adverse-effects induced by most current anticancer drugs, the selective cytotoxicity shown by compounds 1, 2 and 5, in particular that of 1, represents a safety factor that encourages the further development of these quinones as new drugs in cancer therapy.

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Replication: Organization and Replication of the Eukaryotic Chromosome

Nagl

1977

Progress in Botany / Fortschritte Der Botanik

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Satellite DNA sequence content of polylysine-titratable and nuclease-resistant fractions of mouse liver hepatoma chromatin

Cited by 6 publications

References 35 publications

Transferring DNA from electrophoretically resolved nucleosomes to diazobenzyloxymethyl cellulose: properties of nucleosomes along mouse satellite DNA

Transferring DNA from electrophoretically resolved nucleosomes to diazobenzyloxymethyl cellulose: properties of nucleosomes along mouse satellite DNA

An in vitro comparative study with furyl-1,4-quinones endowed with anticancer activities

Replication: Organization and Replication of the Eukaryotic Chromosome

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