‘Satellite DNA transcripts have diverse biological roles in Drosophila’

Kuhn, Gustavo C. S.

doi:10.1038/hdy.2015.12

Cited by 29 publications

(35 citation statements)

References 22 publications

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“…In particular, the presence of A-T stretches would favor stable DNA curvature that would play a relevant role in heterochromatin condensation (Capriglione et al, 1991;Caputo, Giovannotti, Nisi Cerioni, Splendiani, & Olmo, 2009;Ng, Ness, & Carbon, 1986;Radic, Lundgren, & Hamkalo, 1987). Indeed, A-T stretches occur in the IMO-TaqI element and, as also observed by Capriglione et al (1991), the fact that they are conserved across species investigated could indicateous important biological roles can be fulfilled by satDNA transcripts (e.g., Feliciello, Akrap, & Ugarković, 2015; Grewal & Elgin, 2007;Kuhn, 2015), despite satDNAs have been considered as useless genomic elements for a long time (Ohno, 1972;Orgel & Crick, 1980), it is clear that further investigations will be needed to assess the that they possess a functional role. However, given that more and more scientific evidence has been indicating that vari presence of transcripts of this elements that could help explain this remarkable conservation across the phylogeny of lacertid lizards.…”

Section: Discussionmentioning

confidence: 97%

Characterization of a satellite DNA in the genera Lacerta and Timon (Reptilia, Lacertidae) and its role in the differentiation of the W chromosome

et al. 2018

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In this study, IMO-TaqI satDNA, previously isolated in several species of Lacertidae, was isolated and characterized from four species of the genus Lacerta and three of the genus Timon. The aim was to gain further insights into the evolutionary dynamics of this satDNA, its occurrence among lacertids and to understand if it plays any role in sex chromosome evolution in these seven species. The results here obtained highlighted the presence of this repetitive element in the genome of all the species investigated, thus indicating that IMO-TaqI satDNA is evolutionary conserved among a wide variety of lacertids. In addition, this element was found to be very abundant in the constitutive heterochromatin of the W-sex chromosome of the four Lacerta species investigated. The occurrence of IMO-TaqI satDNA on Lacerta heterochromosome suggests that it is involved in the differentiation of the W chromosome by heterochromatinization, and the fact that it is absent in the W of other lacertids investigated seems to confirm that repetitive DNA sequences would remain randomly trapped into the sex chromosomes, undergoing amplification as a consequence of the suppression of recombination.

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Section: Discussionmentioning

confidence: 97%

Characterization of a satellite DNA in the genera Lacerta and Timon (Reptilia, Lacertidae) and its role in the differentiation of the W chromosome

et al. 2018

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“…In some species, it was shown that these repetitive sequences are involved in genetic degeneration, chromatin organization and regulation of expression 36, 57, 59 . This could also be the case in the E .…”

Section: Discussionmentioning

confidence: 99%

High-throughput analysis of the satellitome revealed enormous diversity of satellite DNAs in the neo-Y chromosome of the cricket Eneoptera surinamensis

Palacios-Gimenez

Dias

Lima

et al. 2017

Sci Rep

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Satellite DNAs (satDNAs) constitute large portion of eukaryote genomes, comprising non-protein-coding sequences tandemly repeated. They are mostly found in heterochromatic regions of chromosomes such as around centromere or near telomeres, in intercalary heterochromatin, and often in non-recombining segments of sex chromosomes. We examined the satellitome in the cricket Eneoptera surinamensis (2n = 9, neo-X1X2Y, males) to characterize the molecular evolution of its neo-sex chromosomes. To achieve this, we analyzed illumina reads using graph-based clustering and complementary analyses. We found an unusually high number of 45 families of satDNAs, ranging from 4 bp to 517 bp, accounting for about 14% of the genome and showing different modular structures and high diversity of arrays. FISH mapping revealed that satDNAs are located mostly in C-positive pericentromeric regions of the chromosomes. SatDNAs enrichment was also observed in the neo-sex chromosomes in comparison to autosomes. Especially astonishing accumulation of satDNAs loci was found in the highly differentiated neo-Y, including 39 satDNAs over-represented in this chromosome, which is the greatest satDNAs diversity yet reported for sex chromosomes. Our results suggest possible involvement of satDNAs in genome increasing and in molecular differentiation of the neo-sex chromosomes in this species, contributing to the understanding of sex chromosome composition and evolution in Orthoptera.

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“…Furthermore, we highlight recent studies on insects in which differential transcription was detected at different stages of development, although its function is currently unknown (Lima et al, 2017, among others). Despite the foregoing, and as indicated above, the transcripts of the 1,688 satellite DNA from Drosophila melanogaster seem to have key roles, making their study highly relevant for structural, functional and evolutionary genomics (Kuhn, 2015).…”

Section: Resultsmentioning

confidence: 99%

Characterization and transcriptional analysis of a subtelomeric satellite DNA family in the ladybird beetle Henosepilachna argus (Coleoptera: Coccinellidae)

Mora¹,

Vela²,

Ruiz-Mena³

et al. 2017

Eur. J. Entomol.

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Satellite DNAs are the major repetitive DNA components in eukaryotic genomes. Although satellite DNA has long been called "parasite DNA" there is substantial evidence that it could be associated with some functions of chromosome biology. Ladybird beetles (Coccinellidae) are one of the largest and most important groups of beetles. Many ladybirds are of economic interest as biological control agents because they eat some agricultural pests such as aphids and scale insects. However, other species are phytophagous and can damage crops. Despite the ecological importance of the latter group there are no studies on their satellite DNA. A satellite DNA family was isolated and characterized in the ladybird Henosepilachna argus. This satellite DNA is organized in tandem repeats of 658 bp and is A + T rich (67.3%). The recorded high sequence conservation of the monomers together with the detection of putative gene conversion processes indicate concerted evolution. Reverse transcription polymerase chain reaction (RT-PCR) revealed that this satellite DNA is transcribed and in situ hybridization its location in the subtelomeric regions of all chromosomes except the long arm of the X chromosome. The presence of this satellite DNA in other species of the genus Henosepilachna and Epilachna was also tested using PCR. The results indicate that this satellite DNA sequence is so far specifi c to H. argus.

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‘Satellite DNA transcripts have diverse biological roles in Drosophila’

Cited by 29 publications

References 22 publications

Characterization of a satellite DNA in the genera Lacerta and Timon (Reptilia, Lacertidae) and its role in the differentiation of the W chromosome

Characterization of a satellite DNA in the genera Lacerta and Timon (Reptilia, Lacertidae) and its role in the differentiation of the W chromosome

High-throughput analysis of the satellitome revealed enormous diversity of satellite DNAs in the neo-Y chromosome of the cricket Eneoptera surinamensis

Characterization and transcriptional analysis of a subtelomeric satellite DNA family in the ladybird beetle Henosepilachna argus (Coleoptera: Coccinellidae)

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