SBP1 is an adhesion-associated factor without the involvement of virulence in Streptococcus suis serotype 2

Qian, Yufeng; Zhang, Yuhang; Yu, Yun William; Quan, Li Na; Guo, Genglin; Fu, Yang; Yao, Huochun; Chen, Lu; Zhang, Wei

doi:10.1016/j.micpath.2018.06.008

Cited by 6 publications

(5 citation statements)

References 31 publications

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“…Among these genes, such as rgg (SSU05_1997) (Zheng et al, 2011), type 1 restriction-modification system cluster component hsdS (SSU05_1451) (Xu et al, 2017) was already be studied to be related to the virulence of SS2. Based on this research, two gene cluster, srtBCD gene cluster (SSU05_2096-SSU05_2099) (Qian et al, 2018;Shao, Zhang, Wu, & Lu, 2014;Yu et al, 2016) and an nmt gene cluster (SSU05_1971-SSU05_1973), were proved associated with virulence of SS2 by our group (Li, Zhang, Dechao, Yanfei, & Zhang, 2018).…”

Section: Virulence-associated Gene Identified Through Comparative Ana...mentioning

confidence: 76%

Pan‐genome analysis of Streptococcus suis serotype 2 revealed genomic diversity among strains of different virulence

Guo

et al. 2020

Transbounding Emerging Dis

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Section: Virulence-associated Gene Identified Through Comparative Ana...mentioning

confidence: 76%

Pan‐genome analysis of Streptococcus suis serotype 2 revealed genomic diversity among strains of different virulence

Guo

et al. 2020

Transbounding Emerging Dis

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“…Another 21 previously uncharacterized patient isolates were also positive using these primers (Appendix Table S8). Other published isolates with high virulence and human infecting potential (Yu et al, 2016;Qian et al, 2018) were also tested positive. As an important control, all 10 lowvirulence isolates from pigs were tested negative (Appendix Fig S13).…”

Section: Clade-specific Signatures Contribute To the Virulence Of Human-associated Isolatesmentioning

confidence: 99%

“…In order to confirm if the primer pairs work well in silico, they were tested using a perl script publicly available at https://github.com/egonozer/in_silico_pcr. To further assess the validity of these markers, two groups of S. suis isolates were used in this study: (i) a training collection of 24 isolates (12 isolates from HAC, 6 each from clade B1 and clade B2, respectively) and (ii) an out-of-sample test collection of 43 isolates (21 previously uncharacterized isolates from patient and 22 isolates (21 isolates from pigs and an isolate from patient) with known virulence published before) (Yu et al, 2016;Qian et al, 2018). PCR was performed for 5 min at 95°C, followed by 30 cycles of 30 s at 95°C, 30 s at 55°C, and 2 min at 72°C.…”

Section: Pan-genome Analysis Of Potential Markersmentioning

confidence: 99%

The global emergence of a novel Streptococcus suis clade associated with human infections

et al. 2021

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Streptococcus suis, a ubiquitous bacterial colonizer in pigs, has recently extended host range to humans, leading to a global surge of deadly human infections and three large outbreaks since 1998. To better understand the mechanisms for the emergence of crossspecies transmission and virulence in human, we have sequenced 366 S. suis human and pig isolates from 2005 to 2016 and performed a large-scale phylogenomic analysis on 1,634 isolates from 14 countries over 36 years. We show the formation of a novel human-associated clade (HAC) diversified from swine S. suis isolates. Phylogeographic analysis identified Europe as the origin of HAC, coinciding with the exportation of European swine breeds between 1960s and 1970s. HAC is composed of three sub-lineages and contains several healthy-pig isolates that display high virulence in experimental infections, suggesting healthy-pig carriers as a potential source for human infection. New HAC-specific genes are identified as promising markers for pathogen detection and surveillance. Our discovery of a human-associated S. suis clade provides insights into the evolution of this emerging human pathogen and extend our understanding of S. suis epidemics worldwide.

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“…Considering the essential role of SrtA in the pathogenic mechanisms of Gram-positive bacteria, it is also considered an ideal target for potential drugs (Spirig et al, 2011 ). SrtC sorting enzyme not only anchors smaller substrates but also participates in pilus assembly, suggesting that the formation of pili is an inherent ability of SS2 to promote microbial adhesion and biofilm formation (LeMieux et al, 2008 ; Qian et al, 2018 ; Faulds-Pain et al, 2019 ). In addition, during the process of adhering to endothelial cells and epithelial cells in host tissues, SS2 mediates host cell invasion through its own ECM-binding protein, binding to host ECM components fibronectin (FN) and laminin (LN; Schwarz-Linek et al, 2003 ; Wahid et al, 2005 ; Ragunathan et al, 2009 ).…”

Section: Discussionmentioning

confidence: 99%

Pan-genome analysis of Streptococcus suis serotype 2 highlights genes associated with virulence and antibiotic resistance

Zhou,

Tu,

Yao

et al. 2024

Front. Microbiol.

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Streptococcus suis serotype 2 (SS2) is a Gram-positive bacterium. It is a common and significant pathogen in pigs and a common cause of zoonotic meningitis in humans. It can lead to sepsis, endocarditis, arthritis, and pneumonia. If not diagnosed and treated promptly, it has a high mortality rate. The pan-genome of SS2 is open, and with an increasing number of genes, the core genome and accessory genome may exhibit more pronounced differences. Due to the diversity of SS2, the genes related to its virulence and resistance are still unclear. In this study, a strain of SS2 was isolated from a pig farm in Sichuan Province, China, and subjected to whole-genome sequencing and characterization. Subsequently, we conducted a Pan-Genome-Wide Association Study (Pan-GWAS) on 230 strains of SS2. Our analysis indicates that the core genome is composed of 1,458 genes related to the basic life processes of the bacterium. The accessory genome, consisting of 4,337 genes, is highly variable and a major contributor to the genetic diversity of SS2. Furthermore, we identified important virulence and resistance genes in SS2 through pan-GWAS. The virulence genes of SS2 are mainly associated with bacterial adhesion. In addition, resistance genes in the core genome may confer natural resistance of SS2 to fluoroquinolone and glycopeptide antibiotics. This study lays the foundation for further research on the virulence and resistance of SS2, providing potential new drug and vaccine targets against SS2.

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SBP1 is an adhesion-associated factor without the involvement of virulence in Streptococcus suis serotype 2

Cited by 6 publications

References 31 publications

Pan‐genome analysis of Streptococcus suis serotype 2 revealed genomic diversity among strains of different virulence

Pan‐genome analysis of Streptococcus suis serotype 2 revealed genomic diversity among strains of different virulence

The global emergence of a novel Streptococcus suis clade associated with human infections

Pan-genome analysis of Streptococcus suis serotype 2 highlights genes associated with virulence and antibiotic resistance

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