Scaffold degradation during bone tissue reconstruction in Macaca nemestrina mandible

Bachtiar, Endang W.; Amir, Lisa Rinanda; Suhardi, Pradono; Abas, Basril

doi:10.1556/1646.8.2016.2.5

Cited by 10 publications

(11 citation statements)

References 11 publications

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“…The prognosis for this condition is quite poor owing to the difficulties faced in stabilizing the graft material until regeneration starts [5,13]. An earlier study on M. nemestrina and a human mandible showed the first evidence of improved bone regeneration when using chitosan as a scaffold [4,9]. Furthermore, the use of ligament periodontal cell sheets was found to promote regeneration in an animal study [14,15].…”

Section: Discussionmentioning

confidence: 99%

“…A bone defect with crater defects or a vertical defect with a three-wall bony defect is suitable for the bone grafting procedure [2]. A one-wall bone defect or horizontal bone defect has less healthy cells and a large defect, causing difficulties in the ability of grafts to produce regenerative cells that are similar to or that resemble the lost tissue [4,5]. To overcome this limitation, several combinations of regenerative therapies and tissue engineering applications have been studied.…”

Section: Introductionmentioning

confidence: 99%

“…Chitosan can form an insoluble complex with connective tissue molecules such as collagen and glycosaminoglycan to form a three-dimensional porous structure, making it a good choice for a scaffold material in tissue engineering. An ideal scaffold should have the ability to facilitate new bone formation, and the use of chitosan in a Macaca nemestrina study showed that a chitosan scaffold biodegrades after 4 weeks [4,9,10].…”

Section: Introductionmentioning

confidence: 99%

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Evaluation of Regenerative Therapy Using Cell Sheet Through Cementum Protein-1 Expression on Macaca Nemestrina

et al. 2018

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Methods:The CEMP-1 was analyzed expression in a sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) gel electrophoresis assay.Results: CEMP-1 expression in gingival crevicular fluid was observed using the SDS-PAGE method every week for 3 weeks. Protein band expressions on SDS-PAGE gel were identified at around 50 kDa with different thicknesses between groups. The chitosan, chitosan cell sheet, and RGD-modified chitosan cell sheet groups showed protein bands of CEMP-1 between 50 and 70 kDa at weeks 1, 2, and 3; weeks 2 and 3; and weeks 1 and 2, respectively. Conclusion:Our results demonstrated that the application of chitosan and RGD-modified chitosan cell sheets could enhance bone regeneration, as evidenced by CEMP-1 protein expression levels.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Evaluation of Regenerative Therapy Using Cell Sheet Through Cementum Protein-1 Expression on Macaca Nemestrina

et al. 2018

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“…Cell isolation procedures were as previously described. 14,22 Briefly, PDL tissue was obtained from apical two-thirds of the root. Enzymatic dissociation was performed with 4 mg/mL collagenase I and 3 mg/mL dispase in a 15 mL tube at 37°C under 5% CO 2 in an incubator for 1 hour.…”

Section: Cell Culturementioning

confidence: 99%

Periodontal Ligament Cell Sheets and RGD-Modified Chitosan Improved Regeneration in the Horizontal Periodontal Defect Model

et al. 2020

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Objective The aim of this study was to examine the potential of periodontal ligament (PDL) cells sheet and arginine-glycyl-aspartic acid (RGD)-modified chitosan scaffold for periodontal tissue regeneration in horizontal periodontal defect model. Materials and Methods PDL cell cytotoxicity was tested with 3–[4,5- dimethylthiazol-2yl]–2,5-diphenyl-2H-tetrazolium bromide assay. Cell migration toward the chitosan-based materials was analyzed with trans-well migration assay. Horizontal periodontal defect model was created in four maxillary and mandibular lateral incisors of Macaque nemestrina. Following periodontal therapy, the sites were transplanted with various regenerative materials: (1) chitosan, (2) RGD-modified chitosan, (3) PDL cell sheet with chitosan, (4) PDL cell sheet with RGD-modified chitosan. The periodontal tissue regeneration was evaluated clinically and radiographically. Gingival crevicular fluids were collected each week to evaluate cementum protein-1 (CEMP-1) expression with enzyme-linked immunosorbent assay, while the biopsies were retrieved after 4 weeks for histological and microcomputed tomography evaluation. Statistical Analysis Data was statistically analyzed using GraphPad Prism 6 for MacOS X. Normality was tested using the Shapiro–Wilk normality test. The Kruskal–Wallis test was used to compare the groups. Significance was accepted when p < 0.05. Results Clinical examination revealed more epithelial attachment was formed in the group with PDL cell sheet with RGD-modified chitosan. Similarly, digital subtraction radiography analysis showed higher gray scale, an indication of higher alveolar bone density surrounded the transplanted area, as well as higher CEMP-1 protein expression in this group. The incorporation of RGD peptide to chitosan scaffold in the group with or without PDL cells sheet reduced the distance of cement–enamel junction to the alveolar bone crest; hence, more periodontal tissue formed. Conclusions Horizontal periodontal defect model could be successfully created in M. nemestrina model. Combination of PDL cell sheet and RGD-modified chitosan resulted in the higher potential for periodontal tissue regeneration. The results of this study highlight the PDL cell sheet and RGD-modified chitosan as a promising approach for future clinical use in periodontal regeneration.

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“…Scientists have developed a biopolymer material, known as chitosan, for use in dentistry ( Dutta et al, 2004 , Bachtiar et al, 2016 , Ikono et al, 2019a , Ikono et al, 2019b ). Chitosan is derived from shrimp shells and obtained through partial and full alkaline deacetylation processes and by combining organic and inorganic structures ( Waibel et al, 2011 ).…”

Section: Introductionmentioning

confidence: 99%

Chitosan gel prevents the growth of Porphyromonas gingivalis, Tannerella forsythia, and Treponema denticola in mini-implant during orthodontic treatment

Anggani

Rusli

Bachtiar

2021

The Saudi Dental Journal

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Aims We evaluated the effect of chitosan gel on total oral bacteria, Porphyromonas gingivalis , Tannerella forsythia , and Treponema denticola , during orthodontic treatment with mini-implants. Material and methods Thirty subjects with 52 orthodontic mini-implants were divided into three groups: one group was treated with chitosan gel, the other group with chlorhexidine gel, and the control group with placebo. The plaque of the orthodontic peri -mini-implant area was collected before and after gel treatment. The total oral bacteria and red-complex bacteria of P. pingivalis , T. forsythia , and T. denticola were determined with reverse transcription-quantitative PCR. Results Thirty-four orthodontic mini-implants (65.38%) appeared as healthy and showed no clinical signs of inflammation. The total number of bacteria was reduced after chitosan gel application. The highest decrease in the proportion of P. gingivalis was observed in the chlorhexidine gel application group, which showed a value of 70.86%, whereas the chitosan gel application showed a reduction of only 26.59%, and the control gel application showed the lowest reduction effect of only 2.55%. The difference in the reduction between gel application groups was significant (P < 0.05) for T. denticola and T. forsythia . Conclusion The gel containing chitosan reduced the levels of total oral bacteria and red-complex bacteria.

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Scaffold degradation during bone tissue reconstruction in Macaca nemestrina mandible

Abstract: Degradation of the scaffold did not induce higher inflammatory response compared to the control yet it induced more osteoclast activity.

Cited by 10 publications

References 11 publications

Evaluation of Regenerative Therapy Using Cell Sheet Through Cementum Protein-1 Expression on Macaca Nemestrina

Evaluation of Regenerative Therapy Using Cell Sheet Through Cementum Protein-1 Expression on Macaca Nemestrina

Periodontal Ligament Cell Sheets and RGD-Modified Chitosan Improved Regeneration in the Horizontal Periodontal Defect Model

Chitosan gel prevents the growth of Porphyromonas gingivalis, Tannerella forsythia, and Treponema denticola in mini-implant during orthodontic treatment

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