Scaffold-in-Scaffold Potential to Induce Growth and Differentiation of Cardiac Progenitor Cells

Ciocci, Matteo; Mochi, Federico; Carotenuto, Felicia; Giovanni, Emilia Di; Prosposito, P.; Francini, R.; F, De Matteis; Решетов, И. В.; Casalboni, M.; Melino, Sonia; Nardo, Paolo Di

doi:10.1089/scd.2017.0051

Cited by 27 publications

(25 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In this context, a promising example of the cradle infrastructure is represented by the ‘scaffold‐in‐scaffold’ design, in which a stiffer woodpile microstructure is embedded in a biocompatible hydrogel and the whole structure is soaked into the culture medium. Stem cells are incorporated into the hydrogel, successfully grown and induced to differentiate into cardiomyocytes on the woodpile structure that rudimentarily mimics the organization of the ECM . In the ‘scaffold‐in‐scaffold’ design, a three‐dimensional intricate network of fibres dynamically releases mechano‐structural signals into the cells (Figure ).…”

Section: Supplying Cells With Proper Microenvironmental Cuesmentioning

confidence: 99%

“…The image comes from a set of experiments made in our laboratory. The essential results were published …”

Section: Supplying Cells With Proper Microenvironmental Cuesmentioning

confidence: 99%

“…Stem cells are incorporated into the hydrogel, successfully grown and induced to differentiate into cardiomyocytes on the woodpile structure that rudimentarily mimics the organization of the ECM. 65 In the 'scaffold-in-scaffold' design, a three-dimensional intricate network of fibres dynamically releases mechano-structural signals into the cells (Figure 2). Natural and synthetic materials are under investigation for use in this novel family of structures, but much remains to be examined about their optimal chemistry and design, and, above all, the adaptive strategies utilized by cells when in the presence of unfamiliar materials.…”

Section: Supplying Cell S With Proper Microenvironmental Cue Smentioning

confidence: 99%

“…The image comes from a set of experiments made in our laboratory. The essential results were published 65 turn, modify the organization and composition of the ECM. 66 In this context, cardiac differentiation is propelled by a complex dynamics of biochemical, biophysical and bioelectrical signals.…”

Section: Supplying Cell S With Proper Microenvironmental Cue Smentioning

confidence: 99%

See 3 more Smart Citations

Turning regenerative technologies into treatment to repair myocardial injuries

Carotenuto

Teodori

Maccari

et al. 2019

J Cellular Molecular Medi

Self Cite

View full text Add to dashboard Cite

Regenerative therapies including stem cell treatments hold promise to allow curing patients affected by severe cardiac muscle diseases. However, the clinical efficacy of stem cell therapy remains elusive, so far. The two key roadblocks that still need to be overcome are the poor cell engraftment into the injured myocardium and the limited knowledge of the ideal mixture of bioactive factors to be locally delivered for restoring heart function. Thus, therapeutic strategies for cardiac repair are directed to increase the retention and functional integration of transplanted cells in the damaged myocardium or to enhance the endogenous repair mechanisms through cell‐free therapies. In this context, biomaterial‐based technologies and tissue engineering approaches have the potential to dramatically impact cardiac translational medicine. This review intends to offer some consideration on the cell‐based and cell‐free cardiac therapies, their limitations and the possible future developments.

show abstract

Section: Supplying Cells With Proper Microenvironmental Cuesmentioning

confidence: 99%

“…The image comes from a set of experiments made in our laboratory. The essential results were published …”

Section: Supplying Cells With Proper Microenvironmental Cuesmentioning

confidence: 99%

Section: Supplying Cell S With Proper Microenvironmental Cue Smentioning

confidence: 99%

Section: Supplying Cell S With Proper Microenvironmental Cue Smentioning

confidence: 99%

See 2 more Smart Citations

Turning regenerative technologies into treatment to repair myocardial injuries

Carotenuto

Teodori

Maccari

et al. 2019

J Cellular Molecular Medi

Self Cite

View full text Add to dashboard Cite

show abstract

“…One study used a novel concept called "scaffold-in-scaffold" to promote human CPC growth and differentiation in vitro [258]. The aim of this approach was to create a structure with different physical characteristics to better mimic the ECM microarchitecture.…”

Section: Synthetic Scaffoldsmentioning

confidence: 99%

Cardiac Progenitor Cells from Stem Cells: Learning from Genetics and Biomaterials

Barreto

Hamel

Schiatti

et al. 2019

Cells

View full text Add to dashboard Cite

Cardiac Progenitor Cells (CPCs) show great potential as a cell resource for restoring cardiac function in patients affected by heart disease or heart failure. CPCs are proliferative and committed to cardiac fate, capable of generating cells of all the cardiac lineages. These cells offer a significant shift in paradigm over the use of human induced pluripotent stem cell (iPSC)-derived cardiomyocytes owing to the latter’s inability to recapitulate mature features of a native myocardium, limiting their translational applications. The iPSCs and direct reprogramming of somatic cells have been attempted to produce CPCs and, in this process, a variety of chemical and/or genetic factors have been evaluated for their ability to generate, expand, and maintain CPCs in vitro. However, the precise stoichiometry and spatiotemporal activity of these factors and the genetic interplay during embryonic CPC development remain challenging to reproduce in culture, in terms of efficiency, numbers, and translational potential. Recent advances in biomaterials to mimic the native cardiac microenvironment have shown promise to influence CPC regenerative functions, while being capable of integrating with host tissue. This review highlights recent developments and limitations in the generation and use of CPCs from stem cells, and the trends that influence the direction of research to promote better application of CPCs.

show abstract

Cardiac Regeneration: the Heart of the Issue

2021

Self Cite

View full text Add to dashboard Cite

Purpose of Review The regenerative capacity of the heart is insufficient to compensate for the pathological loss of cardiomyocytes during a large injury, such as a myocardial infarction. Therapeutic options for patients after cardiac infarction are limited: treatment with drugs that only treat the symptoms or extraordinary measures, such as heart transplantation. Cell therapies offer a promising strategy for cardiac regeneration. In this brief review, the major issues in these areas are discussed, and possible directions for future research are indicated. Recent Findings Cardiac regeneration can be obtained by at least two strategies: the first is direct to generate an ex vivo functional myocardial tissue that replaces damaged tissue; the second approach aims to stimulate endogenous mechanisms of cardiac repair. However, current cell therapies are still hampered by poor translation into actual clinical applications. Summary In this scenario, recent advancements in cell biology and biomaterial-based technologies can play a key role to design effective therapeutic approaches.

show abstract

Scaffold-in-Scaffold Potential to Induce Growth and Differentiation of Cardiac Progenitor Cells

Abstract: Stem Cells and Development Scaffold-in-scaffold potential to induce growth and differentiation of cardiac progenitor cells

Cited by 27 publications

References 41 publications

Turning regenerative technologies into treatment to repair myocardial injuries

Turning regenerative technologies into treatment to repair myocardial injuries

Cardiac Progenitor Cells from Stem Cells: Learning from Genetics and Biomaterials

Cardiac Regeneration: the Heart of the Issue

Contact Info

Product

Resources

About