Scaffolds for bone regeneration made of hydroxyapatite microspheres in a collagen matrix

Cholas, Rahmatullah H.; Padmanabhan, Sanosh Kunjalukkal; Gervaso, Francesca; Udayan, Gayatri; Monaco, Graziana; Sannino, Alessandro; Licciulli, Antonio

doi:10.1016/j.msec.2016.03.022

Cited by 68 publications

(42 citation statements)

References 37 publications

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“…Cholas et al [51] embedded spray-dried hydroxyapatite (HA) microspheres within a collagen matrix. HA microspheres presented a mesoporous structure which in combination with the collagen matrix formed a good composite scaffold for human bone tissue engineering.…”

Section: Orthopedic Fieldmentioning

confidence: 99%

Spray Drying: An Overview

Santos¹,

Maurício²,

Sencadas³

et al. 2018

Biomaterials - Physics and Chemistry - New Edition

124

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Spray drying is a well-known method of particle production which comprises the transformation of a fluid material into dried particles, taking advantage of a gaseous hot drying medium, with clear advantages for the fabrication of medical devices. In fact, it is quite common the production of microspheres and microcapsules designed for drug delivery systems. This review describes the different stages of the mechanism of the spray-drying process: atomization, droplet-to-particle conversion and particle collection. In particular, this work addresses the diversity of available atomizers, the drying kinetics and the importance of the configuration of the drying chamber, and the efficiency of the collection devices. The final properties of the dried products are influenced by a variety of factors, namely the spray dryer design, the feed characteristics and the processing parameters. The impact of those variables in optimizing both the spray-drying process and the synthesis of dried particles with desirable characteristics is discussed. The scalability of this manufacturing process in obtaining dried particles in submicron-to-micron scale favors a variety of applications within the food, chemical, polymeric, pharmaceutical, biotechnology and medical industries.

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Section: Orthopedic Fieldmentioning

confidence: 99%

Spray Drying: An Overview

Santos¹,

Maurício²,

Sencadas³

et al. 2018

Biomaterials - Physics and Chemistry - New Edition

124

View full text Add to dashboard Cite

show abstract

“…This allows for an enhanced capability to release growth factors because absorption can occur in the shell and within the hollow core (Fu et al, ). The mesoporous structure of the HA shell can also enhance cell adhesion (Cholas et al, ). Disadvantages of using HA include its low mechanical strength, limiting its use in load bearing bones, and its slow degradation rate (Szpalski et al, ; Zhou & Lee, ).…”

Section: Introductionmentioning

confidence: 99%

Relaxin enhances bone regeneration with BMP‐2‐loaded hydroxyapatite microspheres

Injamuri

Rahaman

Yan

et al. 2020

J Biomedical Materials Res

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Our aims were to 1) evaluate the capacity of hollow hydroxyapatite (HA) microspheres (212–250 μm) to serve as a delivery system for controlled release of BMP‐2 in vitro and 2) examine relaxin as an enhancer of BMP‐2 for bone regeneration. Hollow HA microspheres were converted from borate glass microspheres and characterized using X‐ray diffraction, Fourier‐transform infrared spectroscopy, scanning electron microscopy, and the Brunauer–Emmett–Teller method. The microspheres loaded with BMP‐2 and relaxin were implanted for 6 weeks in Sprague Dawley rats with calvarial defects. BMP‐2 alone in the range up to 1 μg per defect exhibited dose‐dependent bone regeneration while relaxin alone in the range up to 0.25 μg per defect did not promote bone regeneration. When compared with BMP‐2 alone (1 μg per defect), a 50% reduction in the BMP‐2 dose was achieved with the addition of 0.05, 0.1, or 0.25 μg of relaxin per defect. These results show that loading HA microspheres with a combination of relaxin and BMP‐2 can significantly reduce the BMP‐2 dose required to regenerate an equivalent amount of bone.

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“…Composites of HA and collagen have been studied to produce composite scaffolds [13,14]. Gelatin, the cost-effective, denatured form of collagen, has also been combined with HA [15].…”

Section: Introductionmentioning

confidence: 99%

Preparation and Characterization of Porous Hydroxyapatite and Alginate Composite Scaffolds for Bone Tissue Engineering

2018

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Objective: To prepare and characterize composite scaffolds of a hydroxyapatite (HA) and an alginate having high viscosity. Materials and Methods:HA powder was synthesized using wet chemical precipitation, the alginate powder was extracted from the Sargassum duplicatum seaweed, and the HA/alginate composite scaffolds were prepared by freeze-drying. X-ray diffraction and Fourier transform infrared techniques were utilized to characterize the HA and alginate, and electron microscopy was used to evaluate the HA and the HA/alginate composite scaffolds. The HA/alginate composite scaffold obtained from the commercially available HA and alginate powders were employed as a comparison.Results: Synthesized HAs were identified as the HA phase, which contained absorbed water, phosphate, and carbonate groups. The extracted alginate contained the carboxyl, cyclic ether and hydroxyl groups. The scaffolds prepared from the HA and alginate mixture were three-dimensional and containing interconnected pores with a diameter ranging from 150 to 300 µm and pore walls of a composite construction. Conclusion:A three-dimensional scaffold was produced using a freeze-drying method from a composite of HA and the high viscosity alginate solution. The scaffold was highly porous and showed interconnected pores, with a diameter ranging from 150 to 300 µm.

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Scaffolds for bone regeneration made of hydroxyapatite microspheres in a collagen matrix

Cited by 68 publications

References 37 publications

Spray Drying: An Overview

Spray Drying: An Overview

Relaxin enhances bone regeneration with BMP‐2‐loaded hydroxyapatite microspheres

Preparation and Characterization of Porous Hydroxyapatite and Alginate Composite Scaffolds for Bone Tissue Engineering

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