Scaffolds from Self-Assembling Tetrapeptides Support 3D Spreading, Osteogenic Differentiation, and Angiogenesis of Mesenchymal Stem Cells

Alshehri, Salwa; Susapto, Hepi Hari; Hauser, Charlotte

doi:10.1021/acs.biomac.1c00205

Cited by 41 publications

(44 citation statements)

References 107 publications

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“… 2 These biocompatible peptide hydrogels have been shown to have promising applications in various biomedical applications such as tissue engineering, drug delivery, regenerative medicine, microbiology, and biosensing. 3 – 9 …”

Section: Introductionmentioning

confidence: 99%

Peptide nanogels as a scaffold for fabricating dermal grafts and 3D vascularized skin models

et al. 2022

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Millions of people worldwide suffer from skin injuries, which create significant problems in their lives and are costly to cure. Tissue engineering is a promising approach that aims to fabricate functional organs using biocompatible scaffolds. We designed ultrashort tetrameric peptides with promising properties required for skin tissue engineering. Our work aimed to test the efficacy of these scaffolds for the fabrication of dermal grafts and 3D vascularized skin tissue models. We found that the direct contact of keratinocytes and fibroblasts enhanced the proliferation of the keratinocytes. Moreover, the expression levels of TGF-β1, b-FGF, IL-6, and IL-1α is correlated with the growth of the fibroblasts and keratinocytes in the co-culture. Furthermore, we successfully produced a 3D vascularized skin co-culture model using these peptide scaffolds. We believe that the described results represent an advancement in the fabrication of skin tissue equivalent, thereby providing the opportunity to rebuild missing, failing, or damaged parts. Graphical abstract [Formula: see text]

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Section: Introductionmentioning

confidence: 99%

Peptide nanogels as a scaffold for fabricating dermal grafts and 3D vascularized skin models

et al. 2022

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“…The triblock copolymer alone, DOX-encapsulated block copolymer, and free DOX were added to each chamber in duplicate and incubated with the cells for 4 h (DOX concentration for all samples was 4 μg/mL). Cell fixation and staining were done by following a reported protocol . Briefly, the media were removed from each well, and the cells were washed with PBS three times.…”

Section: Methodsmentioning

confidence: 99%

“…Cell fixation and staining were done by following a reported protocol. 36 Briefly, the media were removed from each well, and the cells were washed with PBS three times. After that, the cells were fixed using 4% paraformaldehyde solution for 30 min at room temperature.…”

Section: ■ Introductionmentioning

confidence: 99%

AIE-Based Fluorescent Triblock Copolymer Micelles for Simultaneous Drug Delivery and Intracellular Imaging

et al. 2021

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Fluorescent drug delivery systems have received increasing attention in cancer therapy because they combine drug delivery and bioimaging into a single platform. For example, polymers with aggregation-induced emission (AIE) fluorophores, such as tetraphenylethylene (TPE), have emerged as an elegant choice for drug delivery/bioimaging applications. In this work, we report one-pot sequential organocatalytic ring-opening polymerization of ε-caprolactone (CL) and ethylene oxide (EO) using TPE-(OH) 2 as a difunctional initiator, in the presence of a t-BuP 2 /TEB Lewis pair (catalyst), in THF at room temperature. Two well-defined triblock copolymers with inverse block sequences, TPE-(PCL-b-PEO) 2 and TPE-(PEO-b-PCL) 2 , were synthesized by altering the sequential addition of CL and EO. The physicochemical properties, including hydrodynamic diameter, morphology, and AIE properties of the synthesized amphiphilic triblock copolymers were investigated in aqueous media. The block copolymer micelles were loaded with anticancer drugs doxorubicin and curcumin to serve as drug delivery vehicles. In vitro studies revealed the accelerated drug release at lower pH (5.5), which mimics the tumor microenvironment, different from the physiological pH (7.4). In vitro cytotoxicity studies demonstrated that the neat block copolymer micelles are biocompatible, while drug-loaded micelles exhibited a significant cytotoxic effect in cancer cells. Cellular uptake, examined by confocal laser scanning microscopy, showed that the block copolymer micelles were rapidly internalized by the cells with simultaneous emission of TPE fluorophore. These results suggest that these triblock copolymers can be utilized for intracellular bioimaging.

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“…Ultrashort amphiphilic peptides, a class of peptides containing three to seven amino acids, can self-assemble into a well-defined nanofibrous network, mimicking the native extracellular matrix. 20,21 Such kinds of short peptides have been used in many applications in medicine and tissue engineering, such as bioprinting, 22,23 drug delivery, 24 engineered tissue models, 25,26 and wound healing, 27 which reveal the biocompatibility of peptides. In our previous works, we have reported the photochemical synthesis of size-controlled biocompatible silver nanoparticles in the absence of any chemical reducing agents for antibacterial applications.…”

Section: ■ Introductionmentioning

confidence: 99%

Synthesis and Organization of Gold-Peptide Nanoparticles for Catalytic Activities

2022

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A significant development in the synthesis strategies of metal-peptide composites and their applications in biomedical and bio-catalysis has been reported. However, the random aggregation of gold nanoparticles provides the opportunity to find alternative fabrication strategies of gold-peptide composite nanomaterials. In this study, we used a facile strategy to synthesize the gold nanoparticles via a green and simple approach where they show self-alignment on the assembled nanofibers of ultrashort oligopeptides as a composite material. A photochemical reduction method is used, which does not require any external chemical reagents for the reduction of gold ions, and resultantly makes the gold nanoparticles of size ca. 5 nm under mild UV light exposure. The specific arrangement of gold nanoparticles on the peptide nanofibers may indicate the electrostatic interactions of two components and the interactions with the amino group of the peptide building block. Furthermore, the gold-peptide nanoparticle composites show the ability as a catalyst to degradation of environmental pollutant p-nitrophenol to p-aminophenol, and the reaction rate constant for catalysis is calculated as 0.057 min −1 at a 50-fold dilute sample of 2 mg/mL and 0.72 mM gold concentration in the composites. This colloidal strategy would help researchers to fabricate the metalized bioorganic composites for various biomedical and bio-catalysis applications.

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Scaffolds from Self-Assembling Tetrapeptides Support 3D Spreading, Osteogenic Differentiation, and Angiogenesis of Mesenchymal Stem Cells

Cited by 41 publications

References 107 publications

Peptide nanogels as a scaffold for fabricating dermal grafts and 3D vascularized skin models

Peptide nanogels as a scaffold for fabricating dermal grafts and 3D vascularized skin models

AIE-Based Fluorescent Triblock Copolymer Micelles for Simultaneous Drug Delivery and Intracellular Imaging

Synthesis and Organization of Gold-Peptide Nanoparticles for Catalytic Activities

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