Scaffolds with anisotropic structure for neural tissue engineering

Hu, Yangnan; Zhang, Hui; Wu, Hao; Cheng, Hong; Cai, Jianguo; Chen, Xiaoyan; Xia, Lin; Wang, Huan; Chai, Renjie

doi:10.1016/j.engreg.2022.04.001

Cited by 34 publications

(29 citation statements)

References 73 publications

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“…Any comparison of the current study’s results with other intervention programs for soft tissue injuries to assess the effectiveness of DK gel compared to other established products is hampered by differences in study design, inclusion criteria, duration of treatment, and efficacy assessment methods. Furthermore, in recent years, a variety of biomaterials (e.g., patches-based hydrogels, cryogels, nanofibers) together with their distinct physicochemical features have been extensively investigated and developed in the fields of drug-delivery, tissue engineering, medicine, and public health, including disease diagnosis [ 21 , 41 , 42 , 43 , 44 , 45 , 46 , 47 , 48 , 49 ]. Thereby, a most recent study in rabbits has reported an injectable thermosenstive hydrogel for dual delivery of diclofenac and avastin (an anti-VEGF) to reduce the inflammation of the corneal neovascularization more effectively [ 49 ].…”

Section: Resultsmentioning

confidence: 99%

Formulation and Evaluation of Diclofenac Potassium Gel in Sports Injuries with and without Phonophoresis

Bukhari¹,

Khan

Ishaq

et al. 2022

Gels

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Background: Pain remains a global public heath priority. Phonophoresis, also known as sonophoresis or ultrasonophoresis, is when an ultrasound is used to maximize the effects of a topical drug. Purpose: The objective of this study was to test, in patients injured in sports or accidents (N = 200), the efficacy of diclofenac potassium (DK) 6%, 4%, and 2% formulated gels with and without phonophoresis in comparison with market available standard diclofenac sodium (DS or DN) gel. Methods: The patients were enrolled after informed consent. By using the lottery method, 100 patients were randomly segregated into five groups without phonophoresis and repeated similarly with phonophoresis at a frequency of 0.8 MHz, an intensity of about 1.5 W/cm2, and at continuous mode (2:1). Group-1 was treated with 6% DK gel, group-2 was treated with 4% DK gel, group-3 was treated with 2% DK gel, group-4 was treated with 4% DS gel and group-5 was given control gel three to four times a week for 4 weeks. The patients were screened by using NPRS and WOMAC scales. They were assessed on the baseline, 4th session, 8th session, 12th session, and 16th session. Results: Significant dose-dependently relief was observed in NPRS (Numeric Pain Rating Scale) and the WOMAC (Western Ontario McMaster Osteo-Arthritis) index for pain in disability and stiffness for each group treated with DK gel compared to DS gel. Phonophoresis increased these benefits significantly when used after topical application of DK gel or DS gel, and the dose-dependent effects of DK gel plus phonophoresis were stronger than the dose-dependent effects of DS gel plus phonophoresis. The faster and profounder relief was due to phonophoresis, which allows more penetration of the DK gel into the skin as compared to the direct application of DK gel in acute, uncomplicated soft tissue injury, such as plantar fasciitis, bursitis stress injuries, and tendinitis. In addition, DK gel with phonophoresis was well tolerated. Thus, in this personalized clinical setting, according to the degree of inflammation or injured-induced pain, disability, and stiffness, DK gel 6% with phonophoresis appeared more effective and thus more recommendable than DS gel 6% alone or DS gel 6% combined to phonophoresis.

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Section: Resultsmentioning

confidence: 99%

Formulation and Evaluation of Diclofenac Potassium Gel in Sports Injuries with and without Phonophoresis

Bukhari¹,

Khan

Ishaq

et al. 2022

Gels

View full text Add to dashboard Cite

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“…Several studies reported preparation and successful application of artificial nerve conduits based on electrospun fibrous materials in biological test systems [13,62,63]. However, relatively few works are deploying fiber nanomaterials with fiber diameters of 100 nm due to technical difficulties of manufacturing [42].…”

Section: Discussionmentioning

confidence: 99%

Light-to-Heat Converting ECM-Mimetic Nanofiber Scaffolds for Neuronal Differentiation and Neurite Outgrowth Guidance

2022

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The topological cues of fibrous scaffolds (in particular extracellular matrix (ECM)-mimetic nanofibers) have already proven to be a powerful tool for influencing neuronal morphology and behavior. Remote photothermal optical treatment provides additional opportunities for neuronal activity regulation. A combination of these approaches can provide “smart” 3D scaffolds for efficient axon guidance and neurite growth. In this study we propose two alternative approaches for obtaining biocompatible photothermal scaffolds: surface coating of nylon nanofibers with light-to-heat converting nanoparticles and nanoparticle incorporation inside the fibers. We have determined photoconversion efficiency of fibrous nanomaterials under near infrared (NIR) irradiation, as well as biocompatible photothermal treatment parameters. We also measured photo-induced intracellular heating upon contact of cells with a plasmonic surface. In the absence of NIR stimulation, our fibrous scaffolds with a fiber diameter of 100 nm induced an increase in the proportion of β3-tubulin positive cells, while thermal stimulation of neuroblastoma cells on nanoparticles-decorated scaffolds enhanced neurite outgrowth and promoted neuronal maturation. We demonstrate that contact guidance decorated fibers can stimulate directional growth of processes of differentiated neural cells. We studied the impact of nanoparticles on the surface of ECM-mimetic scaffolds on neurite elongation and axonal branching of rat hippocampal neurons, both as topographic cues and as local heat sources. We show that decorating the surface of nanofibers with nanoparticles does not affect the orientation of neurites, but leads to strong branching, an increase in the number of neurites per cell, and neurite elongation, which is independent of NIR stimulation. The effect of photothermal stimulation is most pronounced when cultivating neurons on nanofibers with incorporated nanoparticles, as compared to nanoparticle-coated fibers. The resulting light-to-heat converting 3D materials can be used as tools for controlled photothermal neuromodulation and as “smart” materials for reconstructive neurosurgery.

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“…Extrusion-based printing can reach a 200 mm resolution, which is lower than laser or inkjet-based systems, but the technology is fast to manufacture and can generate anatomically shaped structures [ 123 , 124 ]. In the print head or collection phase, extruded bioprinting systems rely on distributing the large polymer or hydrogel chains with micropores and locating them through computer-controlled movements [ 125 , 126 ].…”

Section: Methods Of 3d Printing and Its Introductionmentioning

confidence: 99%

Natural Materials for 3D Printing and Their Applications

Chen

Tian

et al. 2022

Gels

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In recent years, 3D printing has gradually become a well-known new topic and a research hotspot. At the same time, the advent of 3D printing is inseparable from the preparation of bio-ink. Natural materials have the advantages of low toxicity or even non-toxicity, there being abundant raw materials, easy processing and modification, excellent mechanical properties, good biocompatibility, and high cell activity, making them very suitable for the preparation of bio-ink. With the help of 3D printing technology, the prepared materials and scaffolds can be widely used in tissue engineering and other fields. Firstly, we introduce the natural materials and their properties for 3D printing and summarize the physical and chemical properties of these natural materials and their applications in tissue engineering after modification. Secondly, we discuss the modification methods used for 3D printing materials, including physical, chemical, and protein self-assembly methods. We also discuss the method of 3D printing. Then, we summarize the application of natural materials for 3D printing in tissue engineering, skin tissue, cartilage tissue, bone tissue, and vascular tissue. Finally, we also express some views on the research and application of these natural materials.

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Scaffolds with anisotropic structure for neural tissue engineering

Cited by 34 publications

References 73 publications

Formulation and Evaluation of Diclofenac Potassium Gel in Sports Injuries with and without Phonophoresis

Formulation and Evaluation of Diclofenac Potassium Gel in Sports Injuries with and without Phonophoresis

Light-to-Heat Converting ECM-Mimetic Nanofiber Scaffolds for Neuronal Differentiation and Neurite Outgrowth Guidance

Natural Materials for 3D Printing and Their Applications

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