2023
DOI: 10.15252/embr.202357162
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Scalable RT‐LAMP‐based SARS‐CoV‐2 testing for infection surveillance with applications in pandemic preparedness

Abstract: Throughout the SARS‐CoV‐2 pandemic, limited diagnostic capacities prevented sentinel testing, demonstrating the need for novel testing infrastructures. Here, we describe the setup of a cost‐effective platform that can be employed in a high‐throughput manner, which allows surveillance testing as an acute pandemic control and preparedness tool, exemplified by SARS‐CoV‐2 diagnostics in an academic environment. The strategy involves self‐sampling based on gargling saline, pseudonymized sample handling, automated R… Show more

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Cited by 9 publications
(4 citation statements)
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References 51 publications
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“…Lou et al performed more than 35 000 such tests, establishing its capability to run amidst challenged supply chains. 154 Kundrod et al developed a quantitative sample-to-answer RT-LAMP test for detecting SARS-CoV-2 from saliva, nasal, or nasopharyngeal swabs, using an extraction-free simple lysis and sample deactivation protocol. 155 The authors piloted a University-wide surveillance testing program covering upto 400 human subjects per day.…”
Section: Seamless Field Adaptation and Establishment Of Efficacymentioning
confidence: 99%
“…Lou et al performed more than 35 000 such tests, establishing its capability to run amidst challenged supply chains. 154 Kundrod et al developed a quantitative sample-to-answer RT-LAMP test for detecting SARS-CoV-2 from saliva, nasal, or nasopharyngeal swabs, using an extraction-free simple lysis and sample deactivation protocol. 155 The authors piloted a University-wide surveillance testing program covering upto 400 human subjects per day.…”
Section: Seamless Field Adaptation and Establishment Of Efficacymentioning
confidence: 99%
“…In this project, a colorimetric assay based on loop-mediated DNA amplification (LAMP, [35]) was carried out on microtiter plates. The app allowed lab technicians to inspect the measured change in pH as function of incubation time, to link curves to wells and to patients, to compare replicates, and to check and, if needed, amend automatic result calling (see [33] for details). Such continuous quality control is vital for reliable medical diagnostics and has to be offered in an easy-to-use manner and quick-to-grasp to avoid mistakes from repetition and fatigue.…”
Section: Apps With Complex User Interfacesmentioning
confidence: 99%
“…Fig. 8 A screenshot of an app that was used as a GUI to perform manual inspection and classification of LAMP testing for SARS-CoV-2 viral RNA [32,33]. The app was used during our SARS-CoV-2 surveillance study [34] and for voluntary testing for COVID-19 infection on campus (University of Heidelberg) in 2020/2021.…”
Section: Linkedcharts For Open Sciencementioning
confidence: 99%
“…LAMP diagnostic assays have also been successfully used as a point-of-care diagnostic tool during the SARS-CoV-2 pandemic [20][21][22].…”
Section: Introductionmentioning
confidence: 99%