2016
DOI: 10.1534/g3.116.032342
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Scan-o-matic: High-Resolution Microbial Phenomics at a Massive Scale

Abstract: The capacity to map traits over large cohorts of individuals—phenomics—lags far behind the explosive development in genomics. For microbes, the estimation of growth is the key phenotype because of its link to fitness. We introduce an automated microbial phenomics framework that delivers accurate, precise, and highly resolved growth phenotypes at an unprecedented scale. Advancements were achieved through the introduction of transmissive scanning hardware and software technology, frequent acquisition of exact co… Show more

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Cited by 81 publications
(136 citation statements)
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“…We precisely phenotyped the complete set of 6,642 designed POLs (median CoV=10%, mean CoV=14%), their F12 haploid parents, the diploid NA and WA founders and their hybrid in a well replicated ( n ≥4) manner, using a high resolution growth phenomics platform designed to minimize noise and bias20. We selected nine physiologically distinct environmental conditions (Supplementary Table 1) that challenged growth to different extents (Supplementary Fig.…”
Section: Resultsmentioning
confidence: 99%
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“…We precisely phenotyped the complete set of 6,642 designed POLs (median CoV=10%, mean CoV=14%), their F12 haploid parents, the diploid NA and WA founders and their hybrid in a well replicated ( n ≥4) manner, using a high resolution growth phenomics platform designed to minimize noise and bias20. We selected nine physiologically distinct environmental conditions (Supplementary Table 1) that challenged growth to different extents (Supplementary Fig.…”
Section: Resultsmentioning
confidence: 99%
“…These 384 controls were interleaved with experiments on pre-culture plates, ensuring equal treatment of controls and experiments. High resolution population size growth curves were obtained using Epson Perfection V700 PHOTO scanners (Epson corporation, UK) and the Scan-o-matic framework20. Scanners were maintained in a 30 °C, high humidity environment that minimized light influx and evaporation.…”
Section: Methodsmentioning
confidence: 99%
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