Scanining Electron Microscopic Evaluation of Chitosan Nanohydroxyapatite Scaffold for Socket Healing in Rabbits With Induced Osteoporosis

Gharib, Hagar; El-Sawa, Afaf A.; Karam, Sahar S.; Hamza, Sara

doi:10.21608/adjalexu.2020.79878

Cited by 3 publications

(1 citation statement)

References 31 publications

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“…(43) The scanning electron micrographs revealed a qualitative increase in the formation of bone in the collagen group compared to the control group. This finding follows a recent study on the effect of scaffolding after teeth extraction (44), where the grafted sockets showed noticeably increased bone formation than the non-grafted sockets.…”

Section: Discussionsupporting

confidence: 91%

Biological effect of collagen scaffolds on rabbit’s socket healing

Hafez,

Koura,

Kawana

et al. 2023

Alexandria Dental Journal

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BACKGROUND:Tooth extraction initiates a series of biological processes, including alveolar bone resorption driven by the inflammatory response; this eventually leads to alveolar bone resorption. Many researchers have focused on controlling this phenomenon and accelerating new bone formation following tooth extraction. Grafting biomaterials in the extraction socket is now the most dependable and successful strategy for reducing post-extraction resorption. Collagen plugs in the sockets might be an option with potentially desirable outcomes. OBJECTIVES: To assess the biological effect of collagen scaffolds on the healing of extraction sockets MATERIALS AND METHODS: 20 healthy male New Zealand rabbits weighing about 2-2.5 kg, aged 14-16 weeks, was used in this study; their right mandibular first premolar was extracted and divided into two groups with 10 each, control group: sockets were left for normal healing without scaffold administration, collagen group: collagen plugs were administered to the socket. All the rabbits' sockets were dissected and processed for histological analysis. A scanning electron microscope was used to observe the surface morphology of the socket, and Energy dispersed x-ray analysis [EDXA] was used to analyze the calcium and phosphate levels. The information gathered was recorded, and statistical analysis was performed. RESULTS:The results (histology, scanning electron microscopy, and Energy dispersive X-ray analysis) revealed that the group with collagen plugs presented with more bone formation than the group with no scaffold placed in the socket. CONCLUSION: According to the findings of this study, collagen plugs used post-extraction in rabbits increased bone formation compared to sockets without plugs.

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Section: Discussionsupporting

confidence: 91%

Biological effect of collagen scaffolds on rabbit’s socket healing

Hafez,

Koura,

Kawana

et al. 2023

Alexandria Dental Journal

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The Application of Nanotechnology in Constructing Scaffolds for Bone Tissue Engineering

et al. 2021

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Comparative biochemical analysis of platelet-rich plasma-chitosan and platelet-rich fibrin-chitosan for treating tibial bone defects in rabbits

Javaid,

Rashid,

Safdar

et al. 2024

Vet World

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Background and Aim: Fracture healing can cause serious complications, both preoperatively and postoperatively, including malunion or non-union. Biomaterials can enhance the fracture healing process. This study aimed to compare platelet-rich plasma (PRP)-chitosan and platelet-rich fibrin (PRF)-chitosan on the basis of biochemical parameters for fracture treatment in rabbits. Materials and Methods: This study involved 12 clinically healthy rabbits. After preparing PRP and PRF, a 3-mm bone defect was created in the tibia of each rabbit. The animals were divided randomly into two groups (A and B). Group A received PRP-Chitosan, and Group B received PRF-Chitosan. Bone healing was assessed using biochemical parameters (calcium [Ca], phosphorus [P], serum alkaline phosphatase [ALP], and osteocalcin [Ocn]) at 2-, 4-, 6-, and 8-week postoperatively. The data were compared using repeated-measures analysis of variance (p < 0.05) with Statistical Package for the Social Sciences statistical software. Results: Group-wise comparison showed no significant differences (p > 0.05) between the groups, except for ALP levels, which were significantly higher in Group B than in Group A (p < 0.05). In the week-wise comparison, there was a significant difference between both groups, as Ca and ALP levels showed significant differences at all weeks postoperatively, whereas Ocn showed a significant difference at 2- and 4-week postoperatively (p < 0.05). However, there was no difference in P levels between the groups at any post-operative week (p > 0.05). Conclusion: Both combinations enhanced bone regeneration. However, PRF-Chitosan is a better combination for bone repair than PRP-Chitosan. There were some limitations of this study, such as a small sample size, only male rabbits were used, and a lack of mechanical testing; these limitations should be addressed in future studies. The insights gained from the present study may open a new approach to the use of a combination of biomaterials for bone healing, which should be further investigated clinically and in other animal models as a future scope. Keywords: chitosan, fractures, platelet-rich plasma, platelet-rich fibrin, rabbits, tibia.

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Scanining Electron Microscopic Evaluation of Chitosan Nanohydroxyapatite Scaffold for Socket Healing in Rabbits With Induced Osteoporosis

Cited by 3 publications

References 31 publications

Biological effect of collagen scaffolds on rabbit’s socket healing

Biological effect of collagen scaffolds on rabbit’s socket healing

The Application of Nanotechnology in Constructing Scaffolds for Bone Tissue Engineering

Comparative biochemical analysis of platelet-rich plasma-chitosan and platelet-rich fibrin-chitosan for treating tibial bone defects in rabbits

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