Scanning Electron Microscope: Advantages and Disadvantages in Imaging Components

Choudhary, Om Prakash; ka, Priyan

doi:10.20546/ijcmas.2017.605.207

Cited by 55 publications

(26 citation statements)

References 5 publications

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“…In addition to improving the quality of the analyzed images, the coating also reduces the thermal damage on the sample due to charge build-up [13]. Components of SEM device for surface magnification of membranes [14]. SEM can measure the thickness of the dense layer too by cutting the membrane and analyzing the cross-section area.…”

Section: Sample Preparationmentioning

confidence: 99%

Microscopy and Spectroscopy Techniques for Characterization of Polymeric Membranes

Alqaheem

Alomair

2020

Membranes

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Polymeric membrane is a proven technology for water purification and wastewater treatment. The membrane is also commercialized for gas separation, mainly for carbon dioxide removal and hydrogen recovery. Characterization techniques are excellent tools for exploring the membrane structure and the chemical properties. This information can be then optimized to improve the membrane for better performance. In this paper, characterization techniques for studying the physical structure such as scanning electron microscopy (SEM), transmission electron microscopy (TEM), and atomic force microscopy (AFM) are discussed. Techniques for investigating the crystal structure such as X-ray diffraction (XRD), small-angle X-ray scattering (SAXS), and wide-angle X-ray scattering (WAXS) are also considered. Other tools for determining the functional groups such Fourier transform infrared spectroscopy (FTIR), Raman spectroscopy, and nuclear magnetic resonance (NMR) are reviewed. Methods for determining the elemental composition such as energy-dispersion X-ray spectroscopy (EDS), X-ray fluorescent (XRF), and X-ray photoelectron spectroscopy (XPS) are explored. The paper also gives general guidelines for sample preparation and data interpretation for each characterization technique.

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Section: Sample Preparationmentioning

confidence: 99%

Microscopy and Spectroscopy Techniques for Characterization of Polymeric Membranes

Alqaheem

Alomair

2020

Membranes

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“…TEM is one the most employed techniques in the characterization of carbon nanomaterials, as it allows the distinction of similar-looking nanomaterials that, otherwise, might be confused when resorting to scanning electron microscopy [ 103 ]. SEM uses focused electron beams, focusing incidental electrons onto the sample, producing electron scattering triggered by the interaction between the electrons and the sample atoms [ 104 ]. TEM and SEM share several features, and both are capable of discerning particle agglomeration to assess if adequate particle dispersion was accomplished.…”

Section: Characterization Methodsmentioning

confidence: 99%

You Don’t Learn That in School: An Updated Practical Guide to Carbon Quantum Dots

2021

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Carbon quantum dots (CQDs) have started to emerge as candidates for application in cell imaging, biosensing, and targeted drug delivery, amongst other research fields, due to their unique properties. Those applications are possible as the CQDs exhibit tunable fluorescence, biocompatibility, and a versatile surface. This review aims to summarize the recent development in the field of CQDs research, namely the latest synthesis progress concerning materials/methods, surface modifications, characterization methods, and purification techniques. Furthermore, this work will systematically explore the several applications CQDs have been subjected to, such as bioimaging, fluorescence sensing, and cancer/gene therapy. Finally, we will briefly discuss in the concluding section the present and future challenges, as well as future perspectives and views regarding the emerging paradigm that is the CQDs research field.

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“…After fixation, tissues were rinsed thoroughly in 0.1 M phosphate buffer to wash off the excess fixative, followed by postfixation in 1% solution of osmium tetraoxide. The tissues were dehydrated by passing through a series of ascending concentrations of ethanol or acetone (Choudhary and Priyanka, 2017). Clearing of tissues was done by xylene or toluene.…”

Section: Processing Of the Samples For Temmentioning

confidence: 99%

Ultrastructural Studies on the Thyroid Gland of Dromedary Camel (Camelus dromedarius)

Singh¹,

Joshi²,

Thanvi³

et al. 2021

IJAR

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Background: The previously reported information on the ultrastructure of the thyroid gland of the camel is meager as compared to other domestic animals; thus, the present study was designed to provide the ultrastructural features of the thyroid gland in the camel. Methods: The thyroid glands were collected from naturally died sixteen camels (n=16) of both sexes from Veterinary Clinical Complex, RAJUVAS, Bikaner, Rajasthan. The transmission electron microscopy (TEM) of the thyroid gland was done at Sophisticated Analytical Instrumental Facility (SAIF), Department of Anatomy, All India Institute of Medical Sciences, New Delhi. The standard protocol of AIIMS, New Delhi, was followed for electron microscopy. Result: The thyroid gland of the camel consisted of thyroid follicles comprised of cuboidal thyroid follicular cells. The follicular cells contained a nucleus with heterochromatin concentrated marginally on the nuclear membrane and the euchromatin was well dispersed in the nucleus with well-marked nucleoli. The nucleus of the follicular cell was rounded. The apical regions of the plasma membrane in follicular cells consisted of microvilli, which were numerous, thinner and finger-like in outline. The mitochondria appeared as round, oval, rod-shaped and dumbbell-shaped. The rough endoplasmic reticulum was visible as elongated, irregular, elliptical cisterns. The Golgi complex was well marked and consisted of flattened sacs, vacuoles and small vesicles. The parafollicular cells were found in small numbers and positioned basally between two follicular cells, close to the basement membrane. It can be concluded that the ultrastructure of the thyroid gland of the camel does not differ from that of other mammalian species.

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Scanning Electron Microscope: Advantages and Disadvantages in Imaging Components

Cited by 55 publications

References 5 publications

Microscopy and Spectroscopy Techniques for Characterization of Polymeric Membranes

Microscopy and Spectroscopy Techniques for Characterization of Polymeric Membranes

You Don’t Learn That in School: An Updated Practical Guide to Carbon Quantum Dots

Ultrastructural Studies on the Thyroid Gland of Dromedary Camel (Camelus dromedarius)

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