Scanning Electron Microscopy Approach for Evaluation of Hair Dyed with Lawsonia inermis Powder: in vitro Study

Bianchi, Serena; Bernardi, Sara; Continenza, Maria Adelaide; Vincenti, Eleonora; Antonouli, Sevastiani; Torge, Diana; Macchiarelli, Guido

doi:10.4067/s0717-95022020000100096

Cited by 9 publications

(5 citation statements)

References 19 publications

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“…While transcriptomic analysis has revealed profound changes in gene expression in embryos cultured under different oxygen concentrations [37], the morphologic effect of oxygen on embryos is not well known, and was first described by our group [5]. To this end, the current detailed ultrastructural analysis provides a deeper understanding of events occurring during development [28,[38][39][40][41], and allows us to establish a partial correlation between the observed phenotype and the transcriptomic assay, with clinical implications that are valuable in several fields [42][43][44][45].…”

Section: Discussionmentioning

confidence: 99%

Pre-Implantation Mouse Embryos Cultured In Vitro under Different Oxygen Concentrations Show Altered Ultrastructures

Belli

Rinaudo

Palmerini

et al. 2020

IJERPH

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Assisted Reproductive Technologies routinely utilize different culture media and oxygen (O2) concentrations to culture human embryos. Overall, embryos cultured under physiological O2 tension (5%) have improved development compared to embryos cultured under atmospheric O2 conditions (20%). The mechanisms responsible for this remain unclear. This study aimed to evaluate the effect of physiologic (5%) or atmospheric O2 (20%) tension on the microscopic ultrastructure of pre-implantation mouse embryos using Transmission Electron Microscopy (TEM). Embryos flushed out of the uterus after natural mating were used as the control. For use as the control, 2-cells, 4-cells, morulae, and blastocysts were flushed out of the uterus after natural fertilization. In vitro fertilization (IVF) was performed using potassium simplex optimized medium (KSOM) under different O2 tensions (5% and 20%) until the blastocyst stage. After collection, embryos were subjected to the standard preparative for light microscopy (LM) and TEM. We found that culture in vitro under 5% and 20% O2 results in an increase of vacuolated shaped mitochondria, cytoplasmic vacuolization and presence of multi-vesicular bodies at every embryonic stage. In addition, blastocysts generated by IVF under 5% and 20% O2 showed a lower content of heterochromatin, an interruption of the trophectodermal and inner cell mass cell membranes, an increased density of residual bodies, and high levels of glycogen granules in the cytoplasm. In conclusion, this study suggests that in vitro culture, particularly under atmospheric O2 tension, causes stage-specific changes in preimplantation embryo ultrastructure. In addition, atmospheric (20%) O2 is associated with increased alterations in embryonic ultrastructure; these changes may explain the reduced embryonic development of embryos cultured with 20% O2.

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Section: Discussionmentioning

confidence: 99%

Pre-Implantation Mouse Embryos Cultured In Vitro under Different Oxygen Concentrations Show Altered Ultrastructures

Belli

Rinaudo

Palmerini

et al. 2020

IJERPH

Self Cite

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“…This analysis allowed the evaluation of the morphological characteristics of the hair (the surface and the inner part through the cross-section) that could be influenced by cosmetic treatments (shampooing, conditioning, dyeing, bleaching, etc.) [5,[47][48][49].…”

Section: Sem Analysismentioning

confidence: 99%

Rice Derivatives in Hair Protecting

Centini,

Signori,

Francescon

et al. 2023

Cosmetics

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The research was carried out on a hair conditioner containing the following rice derivatives individually: rice germ oil, rice germ oil GX-N, and riceterol esters. To evaluate the protective efficacy of the three active ingredients chosen, the following techniques were used: FT-IR, SEM, stress–strain test, and polarized light microscopy analysis. The tests were carried out on natural Caucasian hair. The methodologies were found to be suitable for the evaluation and led to interesting results: the selected ingredients showed good properties in improving the hair. The conditioners containing the active ingredients restored the properties of the hair even when subjected to stress such as irradiation. In this case, the most effective was the rice germ oil GX-N.

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“…Take henna for example, at pH 4.5-6.0, the reduced form of lawsone (2-hydroxy-1,4-naphthoquinone), its main colorant, reacts with the protonated amino groups present in hair keratin fibers (Figure 1a) [32]. Additionally, SEM observation finds that henna dyestuffs might be capable of recovering the cuticle damage and providing a smooth moisture-rich appearance on the dyed hair cuticles [33].…”

Section: Hair Coloring Mechanismsmentioning

confidence: 99%

Recent Advancements in Natural Plant Colorants Used for Hair Dye Applications: A Review

et al. 2022

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There is an on-going demand in recent years for safer and “greener” hair coloring agents with the global consumer awareness of the adverse effects of synthetic hair dyes. The belief in sustainability and health benefits has focused the attention of the scientific community towards natural colorants that serve to replace their synthetic toxic counterparts. This review article encompasses the historical applications of a vast array of natural plant hair dyes and summarizes the possible coloration mechanisms (direct dyeing and mordant dyeing). Current information on phytochemicals (quinones, tannins, flavonoids, indigo, curcuminoids and carotenoids) used for hair dyeing are summarized, including their botanical sources, color chemistry and biological/toxicological activities. A particular focus is given on research into new natural hair dye sources along with eco-friendly, robust and cost-effective technologies for their processing and applications, such as the synthetic biology approach for colorant production, encapsulation techniques for stabilization and the development of inorganic nanocarriers. In addition, innovative in vitro approaches for the toxicological assessments of natural hair dye cosmetics are highlighted.

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Scanning Electron Microscopy Approach for Evaluation of Hair Dyed with Lawsonia inermis Powder: in vitro Study

Cited by 9 publications

References 19 publications

Pre-Implantation Mouse Embryos Cultured In Vitro under Different Oxygen Concentrations Show Altered Ultrastructures

Pre-Implantation Mouse Embryos Cultured In Vitro under Different Oxygen Concentrations Show Altered Ultrastructures

Rice Derivatives in Hair Protecting

Recent Advancements in Natural Plant Colorants Used for Hair Dye Applications: A Review

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