Scanning electron microscopy studies of the vascular pole of the rat glomerulus

Frank, Martina; Kriz, Wilhelm

doi:10.1002/ar.1092040208

Cited by 8 publications

(11 citation statements)

References 6 publications

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“…After perfusion fixa tion, the kidneys were cut and slices were kept in fixative for 2-6 weeks. After washing in 0.1 Mcacodylate buffer (600 mosm/kg HiO with sucrose) the tissue pieces were processed for SEM and TEM following standard procedures [13,14].…”

Section: Morphological Studiesmentioning

confidence: 99%

Multicystic Transformation of Kidneys in Chronic Renal Failure

Mickisch

Bommer

Bachmann

et al. 1984

Nephron

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With real-time sonography, 120 nondialyzed uremic patients prior to hemodialysis, 108 patients on maintenance hemodialysis and 9 patients postdialysis after successful homotransplantation were examined for the presence of renal cysts. Even in incipient renal failure, multiple cysts were demonstrable in some patients (at a serum creatinine of 3 mg/dl in 22% of patients), particularly in patients with analgesic nephropathy. When hemodialysis was started (serum creatinine approximately 10 mg/dl), 35% of the patients had multiple cysts. On hemodialysis, the prevalence, number and size of cysts rose progressively with time. After 8 years of hemodialysis, 92% of the patients had multiple cysts. However, enlargement of the kidneys was observed in only 2/108 patients. No major clinical complications were noted with the possible exception of 1 case of renal cell carcinoma. No correlation was noted between hematocrit and presence or extent of cystic transformation, but the 2 patients with cystic enlargement of the kidneys were polyglobulic. In 8/9 patients after transplantation, cysts were demonstrable in the patient’s own kidneys after a median follow-up of 16 months. On light microscopy, cysts were lined by cuboidal or columnar epithelial cells with frequent papillary or adenomatous proliferations. The cyst lumen was filled with amorphous or lamellated organic material, which exhibited microfibrillar structure on electron microscopy. One kidney examined after ex vivo perfusion fixation showed multiple interconnected cavities on scanning electron microscopy. Ultrastructural studies showed epithelia with either the characteristics of proximal tubular cells (i.e. numerous microvilli, interdigitations and abundant lysosomes or mitochondria) or distal tubular cells (i.e. highly interdigitating processes) or finally collecting duct cells (i.e. no interdigitations and few microvilli).

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Section: Morphological Studiesmentioning

confidence: 99%

Multicystic Transformation of Kidneys in Chronic Renal Failure

Mickisch

Bommer

Bachmann

et al. 1984

Nephron

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“…The branching of afferent arterioles before reaching the glomerulus has not previously been described in any other vertebrate kidney, and both double afferent and double efferent arterioles appear to be much more common in the trout than in most other species which have been studied (Murakami, 1971(Murakami, , 1976. Models of rat glomeruli have suggested that double afferent arterioles may be more common in mammals than previously suspected (Yang and Morrison, 19801, but a more recent examination of empty Bowman's capsules in kidney slices (Frank and Kriz, 1982) confirmed the low occurrence of double efferent arterioles in mammalian kidneys. In trout, double efferent arterioles arose separately from the glomerular capillary knot, rather than as a single vessel dividing close to the glomerulus.…”

Section: Discussionmentioning

confidence: 95%

“…Models of rat glomeruli have suggested that double afferent arterioles may be more common in mammals than previously suspected (Yang and Morrison, 19801, but a more recent examination of empty Bowman's capsules in kidney slices (Frank and Kriz, 1982) confirmed the low occurrence of double efferent arterioles in mammalian kidneys. In trout, double efferent arterioles arose separately from the glomerular capillary knot, rather than as a single vessel dividing close to the glomerulus.…”

Section: Discussionmentioning

confidence: 95%

Renal microvasculature of the rainbow trout, Salmo gairdneri: Scanning electron microscopy of corrosion casts of glomeruli

1985

Anat. Rec.

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Scanning electron microscopy of corrosion casts of blood vessels permits detailed and accurate study of the microcirculation. The present study examined the renal microvasculature of the rainbow trout, Salmo gairdneri. The conventional picture of a glomerulus with one afferent arteriole was common, but glomeruli were often supplied by two afferent arterioles. In the majority of these, the intrarenal artery gave rise to a single afferent arteriole that branched to form two smaller vessels before reaching the glomerulus. Glomeruli with two afferent arterioles that arose independently from the intrarenal artery also occurred. The majority of glomeruli had a single efferent arteriole, but a proportion of glomeruli had two efferent arterioles. Efferent arterioles were smaller in diameter than the afferent arterioles. The glomerular capillaries were arranged in lobules, with few anastomoses between lobules, so that, for glomeruli with two afferent or two efferent arterioles, vascular perfusion and thus filtration within discrete lobules is probable.

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“…Some of the AA are extremely long and they are similar to the 'giant arterioles' described in the human kidney [26]. Glomeruli with double EA occur in numer ous mammalian species [9,41,42,44]; however, their frequency and location has only been shown in the rat kidney [30], where it has been considered that the number of glomeruli with double EA is less than 5% [14], In the polycystic kidneys observed here, the frequency of super ficial glomeruli displaying double EA is greater than in the control kidneys. This fact emphasizes the idea sug gested previously [30] that double EA represent a malfor mation.…”

Section: Discussionmentioning

confidence: 99%

Vascular Architecture Modifications in the Steroid-Induced Polycystic Kidney

Ros¹,

Ojeda²,

García‐Porrero³

1985

Nephron

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This paper presents a study of the renal vascular architecture in polycystic kidneys induced in newborn rabbits by a single injection of methylprednisolone acetate. Ink perfusion and polyester resin corrosion casts were utilized, and the vasculature was studied by light and scanning electron microscopy. Vascular alterations were observed in the two main evolutive stages of the renal polycystosis: the stage of tubular cysts and the stage of glomerular cysts. These alterations were compared with the normal vascular pattern of control kidneys. At the tubular cyst stage the sinusoidal vascular cortical plexus persisted for a long period of time and the superficial cortical glomerular tufts were poorly developed. At the glomerular cyst stage, the vascular architecture of the superficial cortex showed numerous alterations. The glomerular tufts were small and their structural pattern was affected. Frequently the hilum was very wide and the afferent and efferent arterioles were on opposite sides of the glomerular tufts. The afferent arterioles were variable in size and length. Sometimes the glomeruli showed several afferent arterioles. There were numerous cases of double efferent arterioles in the superficial glomeruli of the polycystic kidneys. Various types of aglomerular shunts were observed regardless of the cystic stage or age.These data indicate that, in addition to the cysts, the corticoid-induced polycystic kidneys showed alterations of the renal vascular architecture which are interpreted as a malformation. We suggest, on the basis of these morphologic observations, that the pathogenesis of the polycystic kidneys is not related to the vascular alterations.

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Scanning electron microscopy studies of the vascular pole of the rat glomerulus

Cited by 8 publications

References 6 publications

Multicystic Transformation of Kidneys in Chronic Renal Failure

Multicystic Transformation of Kidneys in Chronic Renal Failure

Renal microvasculature of the rainbow trout, Salmo gairdneri: Scanning electron microscopy of corrosion casts of glomeruli

Vascular Architecture Modifications in the Steroid-Induced Polycystic Kidney

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