Scanning transmitted and reflected light microscopy: A novel microscopy for visualizing biomaterials at interfaces

Elkady, Ashraf S.

doi:10.1016/j.micron.2007.06.004

Cited by 4 publications

(2 citation statements)

References 30 publications

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“…The difference of tissue specimen component can be delineated in sub-millimeter size. 17 The cross dimension from the top of the epidermis to the bottom of the dermis of the keloid tissues examined in this study was several millimeters. To better illustrate the following multiphoton microscopy imaging results of keloid scar, the transmission microscopy image of keloid tissue was recorded.…”

Section: Large Field Image Of Keloid Tissue With Transmission Mode Mi...mentioning

confidence: 84%

Multiphoton microscopy study of the morphological and quantity changes of collagen and elastic fiber components in keloid disease

et al. 2011

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Multiphoton microscopy was used to study the extracellular matrix of keloid at the molecular level without tissue fixation and staining. Direct imaging of collagen and elastin was achieved by second harmonic generation and two-photon excited fluorescence, respectively. The morphology and quantity of collagen and elastin in keloid were characterized and quantitatively analyzed in comparison to normal skin. The study demonstrated that in keloid, collagen content increased in both the upper dermis and the deep dermis, while elastin mostly showed up in the deep dermis and its quantity is higher compared to normal skin. This suggests the possibility that abnormal fibroblasts synthesized an excessive amount of collagen and elastin at the beginning of keloid formation, corresponding to the observed deep dermis, while after a certain time point, the abnormal fibroblast produced mostly collagen, corresponding to the observed upper dermis. The morphology of collagen and elastin in keloid was disrupted and presented different variations. In the deep dermis, elastic fibers showed node structure, while collagen showed obviously regular gaps between adjacent bundles. In the upper dermis, collagen bundles aligned in a preferred direction, while elastin showed as sparse irregular granules. This new molecular information provided fresh insight about the development process of keloid.

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Section: Large Field Image Of Keloid Tissue With Transmission Mode Mi...mentioning

confidence: 84%

Multiphoton microscopy study of the morphological and quantity changes of collagen and elastic fiber components in keloid disease

et al. 2011

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“…Scanning electron microscopy (SEM), transmission electron microscopy (TEM), and optical microscopy are common but important tools used for the study of cell morphology (Elkady, 2007), physiological capacity (Xiang et al, 2001), surface microvilli, etc. (Gerez et al, 2000), and can provide a great deal of information regarding structural characteristics of cells.…”

Section: Introductionmentioning

confidence: 99%

Atomic force microscope study of tumor cell membranes following treatment with anti-cancer drugs

Wang

Wan

Liu

et al. 2009

Biosensors and Bioelectronics

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Advanced microscopy techniques to assess solid-state properties of inhalation medicines

Shur

Price

2012

Advanced Drug Delivery Reviews

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Efficient control and characterisation of the physico-chemical properties of active pharmaceutical ingredients (APIs) and excipients for orally inhaled drug products (OIDPs) are critical to successful product development. Control and reduction of risk require the introduction of a material science based approach to product development and the use of advanced analytical tools in understanding how the solid-state properties of the input materials influence structure and product functionality. The key issues to be addressed, at a microscopic scale, are understanding how the critical quality attributes of input materials influence surface, interfacial and particulate interactions within OIDPs. This review offers an in-depth discussion on the use of advanced microscopy techniques in characterising of the solid-state properties of particulate materials for OIDPs. The review covers the fundamental principles of the techniques, instrumentation types, data interpretation and specific applications in relation to the product development of OIDPs.

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Scanning transmitted and reflected light microscopy: A novel microscopy for visualizing biomaterials at interfaces

Cited by 4 publications

References 30 publications

Multiphoton microscopy study of the morphological and quantity changes of collagen and elastic fiber components in keloid disease

Multiphoton microscopy study of the morphological and quantity changes of collagen and elastic fiber components in keloid disease

Atomic force microscope study of tumor cell membranes following treatment with anti-cancer drugs

Advanced microscopy techniques to assess solid-state properties of inhalation medicines

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