ScAOC1, an allene oxide cyclase gene, confers defense response to biotic and abiotic stresses in sugarcane

Sun, Tingting; Cen, Guangli; You, Chuihuai; Lou, Wenyue; Wang, Zhoutao; Su, Weihua; Wang, Wenju; Li, Damei; Que, Youxiong; Su, Yachun

doi:10.1007/s00299-020-02606-z

Cited by 21 publications

(10 citation statements)

References 47 publications

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Section: Methodsmentioning

confidence: 99%

“…The root, stem pith, leaf + 1 , bud, and epidermis tissues of nine consistent 10-month-old ROC22 plants (the prevalent sugarcane cultivar in mainland China) were collected [ 42 ]. Four-month-old hydroponic ROC22 tissue-cultured plantlets were sprayed with 100 mM ABA, 5 mM SA (containing 0.01% Tween-20, v/v ), 100 μM MeJA (containing 0.1% ethanol and 0.05% Tween-20, v/v ), and 25% PEG 8000 at 28 °C with 16 h light and 8 h darkness [ 42 , 68 ]. The leaves under SA and MeJA treatments were harvested at 0, 3, 12, and 24 h, the leaves under ABA treatment were collected at 0, 3, 6, and 12 h, and the leaves under PEG stress were harvested at 0, 0.5, 3, 6, and 24 h [ 42 , 68 ].…”

Section: Methodsmentioning

confidence: 99%

“…Four-month-old hydroponic ROC22 tissue-cultured plantlets were sprayed with 100 mM ABA, 5 mM SA (containing 0.01% Tween-20, v/v ), 100 μM MeJA (containing 0.1% ethanol and 0.05% Tween-20, v/v ), and 25% PEG 8000 at 28 °C with 16 h light and 8 h darkness [ 42 , 68 ]. The leaves under SA and MeJA treatments were harvested at 0, 3, 12, and 24 h, the leaves under ABA treatment were collected at 0, 3, 6, and 12 h, and the leaves under PEG stress were harvested at 0, 0.5, 3, 6, and 24 h [ 42 , 68 ]. For cold stress, the whole ROC22 plantlets were kept at a low temperature of 4 °C with 16 h light and 8 h darkness for 0, 6, 12, 24, and 48 h [ 42 , 68 ].…”

Section: Methodsmentioning

confidence: 99%

“…hybrid cultivar R570 [ 38 ], and the sugarcane proximal species Sorghum bicolor [ 39 ], respectively. Second, the protein physicochemical properties, chromosome location, evolutionary relationship, protein motif, gene structure, cis -acting elements, tissue-specific expression, and expression profiles of the COI1 gene family under methyl jasmonate (MeJA), cold, drought, and Sporisorium scitamineum stresses were analyzed [ 40 – 42 ]. Third, the full-length sequences of three ShCOI1 genes ( ShCOI1–4 , ShCOI1–5 , and ShCOI1–6 ) were isolated from Saccharum spp.…”

Section: Introductionmentioning

confidence: 99%

“…hybrid cultivar ROC22 using a homologous cloning method. In addition, the real-time quantitative PCR (RT-qPCR) technique was used to analyze the gene expression patterns of ShCOI1–4 , ShCOI1–5 , and ShCOI1–6 under cold, drought, salicylic acid (SA), abscisic acid (ABA), and S. scitamineum stresses [ 42 – 44 ]. This study aims to uncover and identify the COI1 gene family in sugarcane, understand their sequence characteristics and gene expression patterns, and thus provide candidate gene resources for sugarcane resistance molecular breeding.…”

Section: Introductionmentioning

confidence: 99%

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Genome-wide identification and expression analysis of the coronatine-insensitive 1 (COI1) gene family in response to biotic and abiotic stresses in Saccharum

et al. 2022

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Background The coronatine insensitive 1 (COI1) gene is the core member of jasmonate signaling pathway, which is closely related to plant biotic and abiotic resistance. However, there have been no reports on COI1 in sugarcane (Sacharum spp.). Hence, systematically investigating the characteristics of the COI1 multigene family in sugarcane can provide a means to study and manipulate the jasmonic acid signaling pathway. Results A total of 156 COI1 proteins were obtained from the genomes of 19 land plants, while none were obtained from five algae species. A phylogenetic tree demonstrated that these COI1 proteins were classified into four groups, while 31 proteins of SsCOI1 from Saccharum spontaneum, SbCOI1 from Sorghum bicolor, and ShCOI1 from Saccharum spp. hybrid cultivar R570 clustered into three groups. Synteny analysis and duplication patterns revealed that COI1 genes expanded through various genome replication events and could have experienced strong purifying selective pressure during evolution in S. spontaneum, S. bicolor, and R570. An investigation of cis-acting elements suggests that COI1 genes may be involved in plant growth and development and response to various stresses. Expression analysis implied that 21 SsCOI1 genes were constitutively expressed, and had positive responses to drought, cold, and Sporisorium scitamineum stresses with different expression patterns. Among them, seven SsCOI1 haplotype genes may play different roles in response to methyl jasmonate. Furthermore, the ShCOI1–4, ShCOI1–5, and ShCOI1–6 genes were cloned from Saccharum spp. hybrid cultivar ROC22. Real-time quantitative PCR (RT-qPCR) analysis demonstrated that these three ShCOI1 genes had divergent expression profiles in response to salicylic acid, abscisic acid, polyethylene glycol, cold, and S. scitamineum. Conclusions These results suggest that COI1 genes may act in sugarcane growth, development, and response to various stresses via different regulatory mechanisms, which laying a foundation for the functional identification of the sugarcane COI1 gene.

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Section: Methodsmentioning

confidence: 99%