Scattering angle resolved optical coherence tomography for<i>in vivo</i>murine retinal imaging

Gardner, Michael R.; Katta, Nitesh; McElroy, Austin; Baruah, Vikram; Rylander, H. G.; Milner, Thomas E.

doi:10.1117/12.2253367

Cited by 3 publications

(13 citation statements)

References 15 publications

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“…The reference arm includes three lenses for dispersion matching and a mirror pathlength-matched to the mouse's retina. Permission was granted by the publisher to reprint this figure from Gardner et al with minor modifications [19].…”

Section: System Designmentioning

confidence: 99%

“…Recent examples include hand-held OCT retina and anterior segment imaging systems [24,25]. The system reported here is the first OCT system to use dual-axis MEMS mirror scanning for mouse retinal imaging, and first reported in less detail in 2017 [19].…”

Section: D Mems Mirrormentioning

confidence: 99%

“…Light mapped to Pathlength 3 (P3, red) does not travels through the PME central aperture-both to and from the retina. Permission was granted by the publisher to reprint this figure from Gardner et al with minor modifications[19].Mathematically, the additional pathlengths added for each path through any PME are described generally below:Additionalpathlength added for Pathlength 1 ( ) is = ( + ) − ( + ) = 0. (3) Additional pathlength added for Pathlength 2 ( ) is = ( + • ) − ( + ) = ( − 1).…”

mentioning

confidence: 99%

mentioning

confidence: 99%

“…Pathlength 3 contains both high-and low-angle backscattering and is thus degenerate. Permission was granted by the publisher to reprint this figure from Gardner et al with minor modifications[19].…”

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confidence: 99%

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Design Considerations for Murine Retinal Imaging Using Scattering Angle Resolved Optical Coherence Tomography

et al. 2018

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Optical coherence tomography (OCT), an optical imaging approach enabling cross-sectional analysis of turbid samples, is routinely used for retinal imaging in human and animal models of diseases affecting the retina. Scattering angle resolved (SAR-)OCT has previously been demonstrated as offering additional contrast in human studies, but no SAR-OCT system has been reported in detail for imaging the retinas of mice. An optical model of a mouse eye was designed and extended for validity at wavelengths of light around 1310 nm; this model was then utilized to develop a SAR-OCT design for murine retinal imaging. A Monte Carlo technique simulates light scattering from the retina, and the simulation results are confirmed with SAR-OCT images. Various images from the SAR-OCT system are presented and utility of the system is described. SAR-OCT is demonstrated as a viable and robust imaging platform to extend utility of retinal OCT imaging by incorporating scattering data into investigative ophthalmologic analysis.

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Section: System Designmentioning

confidence: 99%

Section: D Mems Mirrormentioning

confidence: 99%

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confidence: 99%

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confidence: 99%

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Design Considerations for Murine Retinal Imaging Using Scattering Angle Resolved Optical Coherence Tomography

et al. 2018

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Scattering Angle Resolved Optical Coherence Tomography Detects Early Changes in 3xTg Alzheimer's Disease Mouse Model

Gardner

Baruah

Vargas

et al. 2020

Trans. Vis. Sci. Tech.

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Clinical intensity-based optical coherence tomographic retinal imaging is unable to resolve some of the earliest changes to Alzheimer's disease (AD) neurons. The aim of this pilot study was to demonstrate that scattering-angle-resolved optical coherence tomography (SAR-OCT), which is sensitive to changes in light scattering angle, is a candidate retinal imaging modality for early AD detection. SAR-OCT signal data may be sensitive to changes in intracellular constituent morphology that are not detectable with conventional OCT. Methods: In this cross-sectional study, retinas of a triple transgenic mouse model of AD (3xTg-AD) were imaged alongside age-matched control mice (C57BL/6J) using SAR-OCT. A total of 32 mice (12 control, 20 3xTg-Ad) at four ages (10, 20, 30, and 45 weeks) were included in this cross-sectional study, and three retinal feature sets (scattering, thickness, and angiography) were examined between the disease and control groups. Results: AD mice had significantly increased scattering diversity (lower SAR-OCT C parameter) at the earliest imaging time (10 weeks). Differences in the C parameter between AD and control mice were diminished at later times when both groups showed increased scattering diversity. AD mice have reduced retinal thickness compared to controls, particularly in central regions and superficial layers. No differences in vascular density or fractional blood volume between groups were detected. Conclusions: SAR-OCT is sensitive to scattering angle changes in a 3xTg-AD mouse model and could provide early-stage biomarkers for neurodegenerative diseases such as AD. Translational Relevance: Clinical OCT systems may be modified to record SAR-OCT images for non-invasive retinal diagnostic imaging of patients with neurodegenerative diseases such as AD. Recent guidelines and criteria for diagnosing AD include a category for a symptomatic, pre-dementia phase termed "mild cognitive impairment due to AD" 4,5 ; however, even in this early disease state, significant pathophysiological changes are observed. 6 In fact, changes in the brain can begin more than 20 years prior to the onset of AD symptoms. 7-9 Early detection of AD is believed to represent the best path forward for

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Scattering-Angle-Resolved Optical Coherence Tomography of a Hypoxic Mouse Retina Model

et al. 2019

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Several studies have noted a correlation between retinal degeneration and traumatic encephalopathy (TE) making the retina a leading candidate for detection and assessment. Scattering-angle-resolved optical coherence tomography (SAR-OCT) is a candidate imaging modality to detect sub-resolution changes in retinal microstructure. SAR-OCT images of murine retinas that experience a hypoxic insult—euthanasia by isoflurane overdose—are presented. A total of 4 SAR-OCT measurement parameters are reported in 6 longitudinal experiments: blood flow volume fraction, total retinal thickness, reflectance index, and scattering angle. As each mouse expires, blood flow volume fraction decreases, total retinal thickness increases, reflectance index decreases, and scattering angle diversity increases. Contribution of the retinal vasculature to scattering angle diversity is discussed. Results of this study suggest the utility of SAR-OCT to measure TE using scattering angle diversity contrast in the retina.

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Scattering angle resolved optical coherence tomography forin vivomurine retinal imaging

Cited by 3 publications

References 15 publications

Design Considerations for Murine Retinal Imaging Using Scattering Angle Resolved Optical Coherence Tomography

Design Considerations for Murine Retinal Imaging Using Scattering Angle Resolved Optical Coherence Tomography

Scattering Angle Resolved Optical Coherence Tomography Detects Early Changes in 3xTg Alzheimer's Disease Mouse Model

Scattering-Angle-Resolved Optical Coherence Tomography of a Hypoxic Mouse Retina Model

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