Schinus terebinthifolius countercurrent chromatography (Part II): Intra-apparatus scale-up and inter-apparatus method transfer

Costa, Fernanda das Neves; Vieira, Mariana Neves; Garrard, Ian; Hewitson, Peter; Jerz, Gerold; Leitão, Gilda Guimarães; Ignatova, Svetlana

doi:10.1016/j.chroma.2016.08.054

Cited by 19 publications

(8 citation statements)

References 17 publications

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“…X n (k, t x ) (22) where Y = y/x is the dimensionless concentration of a compound in the y-phase and t = τF y /V c is the dimensionless time for the y-phase flow (flow start time τ = 0, t = 0); F y is the volumetric flow rate of the y-phase; X n (k,t x ) describes the distribution of the compound K D along the column at the end of the first stage of separation (τ = τ x , t = t x ), which is determined by Equations ( 3) and ( 18)…”

Section: Equations To Simulate the Second Stage Of Closed-loop Recycling Dual-mode Counter-current Chromatography Separations With Specifmentioning

confidence: 99%

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Closed-Loop Recycling Dual-Mode Counter-Current Chromatography with Specified Sample Loading Durations: Modeling of Preparative and Industrial-Scale Separations

Kostanyan

Вошкин

2021

Molecules

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We previously reported on a new counter-current chromatography (CCC) operating mode called closed-loop recycling dual-mode counter-current chromatography (CLR DM CCC), which incorporates the advantages of closed-loop recycling (CLR) and dual-mode (DM) counter-current chromatography and includes sequential separation of compounds in the closed-loop recycling mode with the mobile x-phase and in the inverted-phase counter-current mode with the mobile y-phase. The theoretical analysis of several implementations of this separation method was carried out under impulse sample injection conditions. This study is dedicated to the further development of CLR DM CCC theory applied to preparative and industrial separations, where high-throughput operation is required. Large sample volumes can be loaded via continuous loading within a specified time. To simulate CLR DM CCC separations with specified sample loading durations, equations are developed and presented in “Mathcad” software.

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Section: Equations To Simulate the Second Stage Of Closed-loop Recycling Dual-mode Counter-current Chromatography Separations With Specifmentioning

confidence: 99%

“…Then, the loop is opened and the second separation stage for compounds 3 and 4 in the inverted-phase counter-current mode with the mobile y-phase starts. The elution profiles of these compounds are simulated by Equation (22).…”

Section: Separation Of Complex Mixtures Containing Compounds With Lower and Higher Partition Coefficients Using Closed-loop Recycling Duamentioning

confidence: 99%

Closed-Loop Recycling Dual-Mode Counter-Current Chromatography with Specified Sample Loading Durations: Modeling of Preparative and Industrial-Scale Separations

Kostanyan

Вошкин

2021

Molecules

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“…HPCCC as a hydrodynamic all-liquid system implements a planetary motion of two self-balancing rotating coils equipped with wounded multi-layer Teflon tubing (PTFE tube) as the channels for the chromatographic phases and the separation process. Specifically, HPCCC allows high mobile flow rates and rotation speeds (up to 1600 rpm), resulting in relatively short separation times and good chromatographic peak resolution [35][36][37]. For obtaining a suitable solvent system for the separation of the two stilbenoids, COSMO-RS was used to calculate the so-called liquid-liquid equilibrium (LLE) and solute partition ratios (K D ) for ε-viniferin and resveratrol.…”

Section: Introductionmentioning

confidence: 99%

In Silico-Assisted Isolation of trans-Resveratrol and trans-ε-Viniferin from Grapevine Canes and Their Sustainable Extraction Using Natural Deep Eutectic Solvents (NADES)

Kiene,

Zaremba,

Fellensiek

et al. 2023

Foods

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Grapevine canes are an important source of bioactive compounds, such as stilbenoids. This study aimed to evaluate an in silico method, based on the Conductor-like Screening Model for Real Solvents (COSMO-RS) to isolate stilbenoids from a grapevine cane extract by offline heart-cut high-performance countercurrent chromatography (HPCCC). For the following extraction of resveratrol and ε-viniferin from grapevine canes, natural deep eutectic solvents (NADES) were used as an environmentally friendly alternative to the traditionally used organic solvents. In order to evaluate a variety of combinations of hydrogen bond acceptors (HBAs) and hydrogen bond donors (HBDs) for the targeted extraction of stilbenoids, COSMO-RS was applied. In particular, ultrasonic-assisted extraction using a solvent mixture of choline chloride/1,2-propanediol leads to higher extraction yields of resveratrol and ε-viniferin. COSMO-RS calculations for NADES extraction combined with HPCCC biphasic solvent system calculations are a powerful combination for the sustainable extraction, recovery, and isolation of natural products. This in silico-supported workflow enables the reduction of preliminary experimental tests required for the extraction and isolation of natural compounds.

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“…HMBC spectrum of 5,7-dihydroxychromone 3 . References [ 14 , 20 , 35 , 37 ] are cited in the supplementary materials.…”

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confidence: 99%

A Comparison between High-Performance Countercurrent Chromatography and Fast-Centrifugal Partition Chromatography for a One-Step Isolation of Flavonoids from Peanut Hulls Supported by a Conductor like Screening Model for Real Solvents

et al. 2023

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Peanut hulls (Arachis hypogaea, Leguminosae), which are a side stream of global peanut processing, are rich in bioactive flavonoids such as luteolin, eriodictyol, and 5,7-dihydroxychromone. This study aimed to isolate these flavonoid derivatives by liquid-liquid chromatography with as few steps as possible. To this end, luteolin, eriodictyol and 5,7-dihydroxychromone were isolated from peanut hulls using two different techniques, high-performance countercurrent chromatography (HPCCC) and fast-centrifugal partition chromatography (FCPC). The suitability of the biphasic solvent system composed of n-hexane/ethyl acetate/methanol/water (1.0/1.0/1.0/1.5; v/v/v/v) was determined by the Conductor like Screening Model for Real Solvents (COSMO-RS), which allowed the partition ratio KD-values of the three main flavonoids to be calculated. After a one-step HPCCC separation of ~1000 mg of an ethanolic peanut hull extract, 15 mg of luteolin and 8 mg of eriodictyol were isolated with purities over 96%. Furthermore, 3 mg of 5,7-dihydroxychromone could be isolated after purification by semi-preparative reversed-phase liquid chromatography (semi-prep. HPLC) in purity of over 99%. The compounds were identified by electrospray ionization mass spectrometry (ESI-MS) and nuclear magnetic resonance spectroscopy (NMR).

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Schinus terebinthifolius countercurrent chromatography (Part II): Intra-apparatus scale-up and inter-apparatus method transfer

Abstract: 15Countercurrent chromatography (CCC) is being widely used across the world for purification

Cited by 19 publications

References 17 publications

Closed-Loop Recycling Dual-Mode Counter-Current Chromatography with Specified Sample Loading Durations: Modeling of Preparative and Industrial-Scale Separations

Closed-Loop Recycling Dual-Mode Counter-Current Chromatography with Specified Sample Loading Durations: Modeling of Preparative and Industrial-Scale Separations

In Silico-Assisted Isolation of trans-Resveratrol and trans-ε-Viniferin from Grapevine Canes and Their Sustainable Extraction Using Natural Deep Eutectic Solvents (NADES)

A Comparison between High-Performance Countercurrent Chromatography and Fast-Centrifugal Partition Chromatography for a One-Step Isolation of Flavonoids from Peanut Hulls Supported by a Conductor like Screening Model for Real Solvents

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