Schinus terebinthifolius essential oil and fractions in the control of Aedes aegypti

Abstract

Several technologies have been developed to control Aedes aegypti, mainly studies on isolated plant molecules. The Schinus terebinthifolius (Raddi) (Anacardiaceae), popularly known as pink pepper is a plant widely used in reforestation of degraded areas and its fruits are used as condiments. The objective of this work was to investigate the potential of essential oils (EOs) and fractions (FRs) obtained from fresh fruits and leaves of S. terebinthifolius. The EOs were obtained by hydrodistillation (2 hours), fractionated on a chromatographic column using as the stationary phase silica gel 60 (0.063-0.2mm), mobile phases: n-hexane, dichloromethane, ethyl acetate and methanol and chemically evaluated by gas chromatography coupled to mass spectrometer (GC/MS). EOs and FRs were tested against larvae of the third stage and pupae of Ae. aegypti by Immersion Test at concentrations ranging from 500.00 to 0.003 mg mL-1 (v/v). The hexane FRs obtained from fruits and leaves were the ones that showed the greatest activity on the larvae (LC99.9= 0.60 mg mL-1 and LC99.9 0.64 mg mL-1, respectively) and pupae (LC99,9 = 2.51 mg mL-1 and 2.61 mg mL-1, respectively). These results were confirmed by the anticholinesterase activity where the hexane (fruit and leaf) FRs presented the highest inhibitory potential on the acetylcholinesterase enzyme (0.156 mg mL-1 and 0.312 mg mL-1, respectively), suggesting the likely mechanism of action. The larvicidal potential can be explained by the presence of the major compounds bicyclogermacrene and germacrene D in the hexane FRs, indicating in this way that they may replace or even act in synergisms with conventional chemical larvicides. In this way the present study opens the field for new researches, aiming the development of products with the compounds bicyclogermacrene and germacrene D, as an alternative in the control of this culicide.

The essential oil ofSchinus terebinthifoliusand its nanoemulsion and isolated monoterpenes: investigation of their activity againstCulex pipienswith insights into the adverse effects on non‐target organisms

“…pipiens . In related studies, crude EO of leaves, fruits and seeds of S. terebinthifolius exhibited pronounced mosquitocidal properties against Cx pipiens , 38 Aedes aegypti , 8, 22, 23, 42 and the malarial vectors Anopheles gambiae s.s ., An. arabiensis and filariasis vector, Culex quinquefasciatus 7 .…”

Análise comparativa do perfil de compostos orgânicos voláteis de pimenta rosa e de aroeira do sertão

“…et al, 2021. As vantagens desse método de extração estão associadas ao seu baixo custo analítico, simplicidade, ausência de solventes e pela alta capacidade de adsorção de diferentes classes de compostos resultante dos diversos tipos de revestimentos das fibras SPME e podendo ser empregado para analisar matrizes complexas como acerola (GARCÍA et al, 2019), cagaita (RODRIGUES et al, 2021SILVA et al, 2019;SILVA et al, 2020;), callistemo (OLIVEIRA JÚNIOR, 2020, cambuí (GARCÍA et al, 2021), cerveja (PEREIRA et al 2021), grumixama (RAMOS et al, 2020RAMOS et al, 2021), kiwi (ASSUNÇÃO et al, 2020, manga (RAMOS, et al, 2021a, RAMOS et al, 2021b, milho (BOTTI et al, 2019;FRANZIN et al, 2020;NASCIMENTO, P. T. et al, 2021), pequi (SANTOS et al, 2020), pera do cerrado (MARIANO et al, 2020), soja (ROCHA. et al, 2019;BUENO et al, 2021), uva (MAZZINGHY, A. C. et al, 2021VIANA et al, 2018).…”

Potential of Tetradenia riparia leaf essential oil and its fractions in controlling Aedes aegypti and Rhipicephalus microplus larvae

“…However, R. microplus and A. aegypti have developed resistance to the major classes of products currently available, necessitating novel products with different modes of action (Manjarres-Suarez and Olivero-Verbel 2013; Zara et al 2016;Banumathi et al 2017). The use of medicinal plants is an interesting alternative in developing countries such as Brazil, underscoring the importance of studies to discover plants with insecticidal effects (Dias and Moraes 2014;Ellse and Wall 2014;Banumathi et al 2017;Muturi et al 2019;Bortolucci et al 2019;Oliveira et al 2019).…”