Schwann cell-derived EVs facilitate dental pulp regeneration through endogenous stem cell recruitment via SDF-1/CXCR4 axis

Wang, Dianri; Lyu, Yun; Yang, Yiqin; Zhang, Sicheng; Pan, Jian; Tian, Weidong

doi:10.1016/j.actbio.2021.11.039

Cited by 34 publications

(23 citation statements)

References 60 publications

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“…It has been reported that several chemokines contribute to recruiting MSCs to the surface/interface of implantation sites; examples include stromal cell-derived factor-1α (SDF-1α)/ CXCR4 and CXCL13/CXCR13 [38,39]. Among these, SDF-1α acts as a powerful chemoattractant for MSC migration from the stem cell niche to the injured site and participates in endogenous endochondral ossification.…”

Section: 4mentioning

confidence: 99%

Engineering Stem Cell Recruitment and Osteoinduction via Bioadhesive Molecular Mimics to Improve Osteoporotic Bone-Implant Integration

Bai

Wang

et al. 2022

Research

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For patients with osteoporosis, the therapeutic outcomes of osteoimplants are substantially affected by the impaired proliferation, migration, and osteogenic differentiation abilities of bone marrow mesenchymal stem cells (BMSCs). To improve bone-implant integration in osteoporotic condition, here we reported a one-step biomimetic surface strategy to introduce BMSC recruiting and osteoinductive abilities onto metallic osteoimplants. In our design, the bioadhesive molecular peptide mimic inspired by mussel foot proteins (Mfps) was used as molecular bridging for surface functionalization. Specifically, a BMSC-targeting peptide sequence (E7) and an osteogenic growth peptide (Y5) were grafted onto the titanium implant surfaces through a mussel adhesion mechanism. We found that a rational E7/Y5 feeding ratio could lead to an optimal dual functionalization capable of not only significantly improving the biocompatibility of the implant but also enabling it to recruit endogenous BMSCs for colonization, proliferation, and osteogenic differentiation. Mechanistically, the E7-assisted in situ recruitment of endogenous BMSCs as well as the enhanced interfacial osteogenesis and osteointegration was associated with activation of the C-X-C chemokine receptor type 4 (CXCR4) receptor on the cell surface and promotion of stromal cell-derived factor (SDF-1α) autocrine secretion. We anticipated that rational dual-functional surfaces through bioadhesive molecular mimics will provide a simple, effective, nonimmunogenic, and safe means to improve the clinical outcomes of intraosseous implants, especially under osteoporotic conditions.

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Section: 4mentioning

confidence: 99%

Engineering Stem Cell Recruitment and Osteoinduction via Bioadhesive Molecular Mimics to Improve Osteoporotic Bone-Implant Integration

Bai

Wang

et al. 2022

Research

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“…41,42 As for the application of EVs for dentin regeneration, several studies revealed that exosomes derived from human dental pulp stem cells (DPSCs) and SCAPs could promote dentin-pulp complex regeneration and odontogenic differentiation of DPSCs through TGFβ1/smads and P38/MAPK signaling via transferring microRNAs. [43][44][45] Zhang and Wang et al 46,47 also reported that EVs derived from Hertwig's epithelial root sheath cells and Schwann cell were able to promote dentin-pulp complex regeneration. Others explored that DPCs-EV combined with treated dentin matrix (TDM) proteins or the control released vehicle as a bioactive pulpcapping material for reparative dentin formation.…”

Section: Discussionmentioning

confidence: 99%

Extracellular vesicles delivering nuclear factor I/C for hard tissue engineering: Treatment of apical periodontitis and dentin regeneration

et al. 2022

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Apical periodontitis (AP) causes arrest of tooth root development, which is associated with impaired odontoblastic differentiation of stem cells from apical papilla (SCAPs), but the underlying mechanism remains unclear. Here, we investigated roles of extracellular vesicle (EV) in AP and odontoblastic differentiation of SCAPs, moreover, a novel nuclear factor I/C (NFIC)-encapsulated EV was developed to promote dentin regeneration. We detected a higher expression of EV marker CD63 in inflamed apical papilla, and found that EVs from LPS-stimulated dental pulp cells suppressed odontoblastic differentiation of SCAPs through downregulating NFIC. Furthermore, we successfully constructed the NFIC-encapsulated EV by overexpressing NFIC in HEK293FT cells, which could upregulate cellular NFIC level in SCAPs, promoting the proliferation and migration of SCAPs, as well as dentinogenesis both in vitro and in vivo. Collectively, based on pathological roles of EV in AP, our study provides a novel strategy for dentin regeneration by exploiting EV to deliver NFIC.

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“…Previous studies have shown that transplantation of DPSCs can promote neurite extension and neuron growth during pulp regeneration, but little is known about the neurogenesis capability of EVs for pulp regeneration [ 11 , 97 ]. EVs have been reported to improve neural recovery and induce neurogenesis in some neural diseases [ 98 ]. Schwann cells (SCs) play a vital role in the support, maintenance, and regeneration of nerve fibers in dental pulp [ 99 ].…”

Section: Evs In Pulp Regenerationmentioning

confidence: 99%

“…Schwann cells (SCs) play a vital role in the support, maintenance, and regeneration of nerve fibers in dental pulp [ 99 ]. Recently, Wang et al revealed that EVs derived from SCs (SC-EVs) enhanced neurite outgrowth and neuron migration of rat dorsal root ganglia explants after coculture, suggesting that SC-EVs are able to promote neural regeneration and might facilitate the regeneration of functional nerve fibers in dental pulp tissue [ 98 ]. To investigate the role of EVs from the dental pulp tissue (DPT-EVs), Chen et al built an in vivo “cell homing” model by filling the root canal of swine teeth with a mixture of treated dental matrix and DPT-EV-laden scaffolds.…”

Section: Evs In Pulp Regenerationmentioning

confidence: 99%

Extracellular Vesicles for Dental Pulp and Periodontal Regeneration

Lai

Kou

et al. 2023

Pharmaceutics

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Extracellular vesicles (EVs) are lipid bound particles derived from their original cells, which play critical roles in intercellular communication through their cargoes, including protein, lipids, and nucleic acids. According to their biogenesis and release pathway, EVs can be divided into three categories: apoptotic vesicles (ApoVs), microvesicles (MVs), and small EVs (sEVs). Recently, the role of EVs in oral disease has received close attention. In this review, the main characteristics of EVs are described, including their classification, biogenesis, biomarkers, and components. Moreover, the therapeutic mechanism of EVs in tissue regeneration is discussed. We further summarize the current status of EVs in pulp/periodontal tissue regeneration and discuss the potential mechanisms. The therapeutic potential of EVs in pulp and periodontal regeneration might involve the promotion of tissue regeneration and immunomodulatory capabilities. Furthermore, we highlight the current challenges in the translational use of EVs. This review would provide valuable insights into the potential therapeutic strategies of EVs in dental pulp and periodontal regeneration.

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Schwann cell-derived EVs facilitate dental pulp regeneration through endogenous stem cell recruitment via SDF-1/CXCR4 axis

Cited by 34 publications

References 60 publications

Engineering Stem Cell Recruitment and Osteoinduction via Bioadhesive Molecular Mimics to Improve Osteoporotic Bone-Implant Integration

Engineering Stem Cell Recruitment and Osteoinduction via Bioadhesive Molecular Mimics to Improve Osteoporotic Bone-Implant Integration

Extracellular vesicles delivering nuclear factor I/C for hard tissue engineering: Treatment of apical periodontitis and dentin regeneration

Extracellular Vesicles for Dental Pulp and Periodontal Regeneration

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