Scientific approach for ozone absorption in blood during systemic indirect endovenous ozonetherapy

Abstract

Introduction.
 Reading reference ozone books from Dra. Menendez, Dra. Viebhan, Dra. Borrelli and Dr. Bocci, proper timing for mixing ozone in blood during autohemotherapy is not calculated in a scientific way, having only an estimation of it based on changes in the blood color, more related to oxygen absorption than on ozone itself.
 
 Material and methods.
 We decided to reproduce a reduced model of great autohemotherapy or recently renamed as systemic indirect endovenous ozonotherapy (SIEVO) by the World Federation of Ozone Therapy – WFOT, using syringes to simplify the experiment. Our model consisted of a 20 mL syringe filled with 10 mL of blood withdrawn from healthy volunteers and mixed it gently but in controlled way with 10 mL medical ozone at different concentrations; after 5 and 8 seconds, the remaining gas was analyzed by an spectrophotometer based ozone detector to check the amount of ozone. Data were analyzed using a linear regression model. 
 
 Results.
 Results show that even for 60 mcgr/mL ozone concentration, 8 seconds is enough to let all ozone absorbed in blood.
 
 Discussion and Conclusions.
 The experiment shows how quick ozone reacts with blood and claims for a trial with real SIEVO devices to achieve a real timing.

Ozone and its derivatives in veterinary medicine: A careful appraisal

“…For homogeneity's sake, the terms “ozonation” and “ozonated” were used in this article. Although the World Federation of Ozone Therapy recently suggested to rename the technique of autohemotherapy as systemic indirect endovenous ozonotherapy (SIEVO) ( Baeza-Noci et al., 2018 ), the former it is still very popular and it will therefore be used in this article.…”

The Relationship between Ozone and Human Blood in the Course of a Well-Controlled, Mild, and Transitory Oxidative Eustress

“…The aim of this paper is to appraise the molecular basis of autohemotherapy, a treatment that involves drawing the patient’s blood and re-administering it back into the patient after the contact with an appropriate dose of a gaseous mixture of oxygen-ozone under clearly defined conditions. For international homogeneity requirements, we will continue to use the term major-autohemotherapy and its acronym M-AHT in the case of intravenous reinfusion of ozone-treated blood, although the definition of systemic intravenous therapy by ex vivo ozonation would be the most accurate and appropriate [ 34 ].…”