Scoring sperm morphology using the scanning electron microscope

Liakatas, J.; Williams, Anthony E.; Hargreave, T. B.

doi:10.1016/s0015-0282(16)46464-2

Cited by 27 publications

(11 citation statements)

References 7 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The smooth apposition of the acrosomal membrane to the head is seen. This smooth covering is commonly observed in normal human spermatozoa (Liakatas et al -(1982) but it can also be somewhat wrinkled and disorganized. Furthermore, disorganization of swim-up head membrane was not seen in washed swim-up spermatiozoa.…”

Section: Resultsmentioning

confidence: 81%

“…Motile swim-up spermatozoa were harvested after two hours from the supernatant and a small fraction of it (0.1 ml) was pelleted in a microcentrifuge tube at 500 x g for 5 minutes. The supernatant was discarded and an electron microscopic fixative (EMF) was layered over sperm pellets, resuspended, fixed overnight at 4 "C and prepared for SEM as previously described (Liakatas et al -(1982). Unwashed semen were fixed similarly and processed for SEM.…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Scanning Electron Microscopic Evaluation of Human Spermatozoa Subjected to Sperm Wash and Swim-Up

et al. 2009

View full text Add to dashboard Cite

Section: Resultsmentioning

confidence: 81%

Section: Methodsmentioning

confidence: 99%

Scanning Electron Microscopic Evaluation of Human Spermatozoa Subjected to Sperm Wash and Swim-Up

et al. 2009

View full text Add to dashboard Cite

“…Ultrastructural analysis of sperms by scanning electron microscopy has been proposed by several authors (Gopalkrishnan et al, 1990;Liakatas et al, 1982;Vijayakumar et al, 1987). The disadvantages of this method are small sample volume, limited observation field, as well as complicated and time consuming preparation techniques.…”

Section: Discussionmentioning

confidence: 99%

“…The disadvantages of this method are small sample volume, limited observation field, as well as complicated and time consuming preparation techniques. However, the benefit this technique offers is that it permits detection of discrete damage to cell surfaces which cannot be achieved with light microscopy or transmission electron microscopy (Liakatas et al, 1982).…”

Section: Discussionmentioning

confidence: 99%

Scanning electron microscopy of human sperms after preparation of semen for in-vitro fertilization

Grab

Thierauf

Rosenbusch

et al. 1993

Arch Gynecol Obstet

View full text Add to dashboard Cite

Ultrastructural changes of human sperms after routine preparation for in-vitro fertilization were examined by scanning electron microscopy. Studies were performed with freshly ejaculated semen of 21 normozoospermic patients. Spermatozoa were analysed at 10000-fold (sperm head with acrosome, postacrosomal border and postnuclear cap) and 2500-fold (midpiece and endpiece of sperm tail) magnification. Compared with untreated specimens, slight membrane damage was found after routine washing and centrifugation procedures in swim-up preparations. However, on the basis of a score system for quantification of morphologic data, no statistically significant differences existed between untreated semen and swim-up preparations. We conclude that, with normozoospermic semen, the rate of ultrastructural damage attributable to sperm-washing procedures is too low to be of clinical consequence.

show abstract

“…The ability to properly assess morphological sperm features mostly depends on pcrsonal experience and the protocol used in the particular laboratory. Different approaches to determine normal sperm morphology have been described (MacLeod & Gold, 1951;Liakatas et al, 1982). It is difficult to indicate the critical spcrm morphological features that are responsible for fertility potential; therefore the main point of discussion is what a particular feature of sperm morphology may tell a rater in a laboratory or clinic.…”

Section: Introductionmentioning

confidence: 99%

Teratozoospermia and its effect on male fertility potential

Szczygiel¹,

Kurpisz²

1999

Andrologia

View full text Add to dashboard Cite

Teratozoospermia and its significance for male fertility problems is discussed. Different points of view and available information concerning the phenomenon of teratozoospermia have been presented. Ongoing debate about the influence of sperm morphology on male infertility and IVF results emphasizes the fact that when sperm morphology is evaluated using strict criteria, this parameter is thought to have an excellent predictive value and shows a significant positive correlation with successful fertilization. Selective abilities of zona pellucida to differentiate between morphologically normal and abnormal spermatozoa, the superior role of acrosomal morphology assessment in the prediction of IVF outcome, and the involvement of cell surface molecules in the adhesion of spermatozoa to oocytes are described. The usefulness of the hamster oocyte penetration assay (HOPA), which is important not only because of its predictive value of sperm function, but also because of its role as a tool for visualizing sperm chromosomes, was found to be questionable in cases of teratozoospermia. Cytogenetic findings related to teratozoospermia are controversial as classical methodology of visualizing sperm chromosomes (through HOPA) may cause selection of sperm metaphases which can be obtained only from good-penetrating spermatozoa. There are few reports presenting chromosome complements of abnormal sperm visualized with the help of micromanipulation. There is clear evidence that a variety of genetic defects can affect spermatogenesis. The possible genetic background of teratozoospermia is discussed.

show abstract

Scoring sperm morphology using the scanning electron microscope

Cited by 27 publications

References 7 publications

Scanning Electron Microscopic Evaluation of Human Spermatozoa Subjected to Sperm Wash and Swim-Up

Scanning Electron Microscopic Evaluation of Human Spermatozoa Subjected to Sperm Wash and Swim-Up

Scanning electron microscopy of human sperms after preparation of semen for in-vitro fertilization

Teratozoospermia and its effect on male fertility potential

Contact Info

Product

Resources

About