<scp>EAP45</scp> association with budding <scp>HIV</scp>‐1: Kinetics and domain requirements

Meng, Bo; Ramirez, Pedro P. Vallejo; Scherer, Katharina M.; Bruggeman, Ezra; Kenyon, Julia C.; Kaminski, Clemens F.; Lever, Andrew

doi:10.1111/tra.12820

Cited by 3 publications

(2 citation statements)

References 57 publications

(122 reference statements)

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“…The signal transducing adapter molecule ( STAM ) gene forms the endosomal sorting complex required for transport-0 (ESCRT-0), and vacuolar protein sorting-associated protein 37B ( VPS37B ) is a component of the ESCRT-I complex. All the four genes are components of the ESCRT complex, and several viruses take advantage of the ESCRT system for proliferation, budding, and transmission in infected cells ( Ju et al, 2021 ; Meng et al, 2021 ). Consequently, we supposed the infection of SARS-CoV-2 may be allied to the ESCRT system.…”

Section: Discussionmentioning

confidence: 99%

Transcriptome Analysis Reveals Hub Genes Regulating Autophagy in Patients With Severe COVID-19

et al. 2022

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Background: The COVID-19 pandemic has currently developed into a worldwide threat to humankind. Importantly, patients with severe COVID-19 are believed to have a higher mortality risk than those with mild conditions. However, despite the urgent need to develop novel therapeutic strategies, the biological features and pathogenic mechanisms of severe COVID-19 are poorly understood.Methods: Here, peripheral blood mononuclear cells (PBMCs) from four patients with severe COVID-19, four patients with mild COVID-19, and four healthy controls were examined by RNA sequencing (RNA-Seq). We conducted gene expression analysis and Venn diagrams to detect specific differentially expressed genes (DEGs) in patients with severe disease compared with those with mild conditions. Gene Ontology (GO) enrichment analysis was performed to identify the significant biological processes, and protein–protein interaction networks were constructed to extract hub genes. These hub genes were then subjected to regulatory signatures and protein–chemical interaction analysis for certain regulatory checkpoints and identification of potent chemical agents. Finally, to demonstrate the cell type-specific expression of these genes, we performed single-cell RNA-Seq analyses using an online platform.Results: A total of 144 DEGs were specifically expressed in severe COVID-19, and GO enrichment analysis revealed a significant association of these specific DEGs with autophagy. Hub genes such as MVB12A, CHMP6, STAM, and VPS37B were then found to be most significantly involved in the biological processes of autophagy at the transcriptome level. In addition, six transcription factors, including SRF, YY1, CREB1, PPARG, NFIC, and GATA2, as well as miRNAs, namely, hsa-mir-1-3p, and potent chemical agents such as copper sulfate and cobalt chloride, may cooperate in regulating the autophagy hub genes. Furthermore, classical monocytes may play a central role in severe COVID-19.Conclusion: We suggest that autophagy plays a crucial role in severe COVID-19. This study might facilitate a more profound knowledge of the biological characteristics and progression of COVID-19 and the development of novel therapeutic approaches to achieve a breakthrough in the current COVID-19 pandemic.

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Section: Discussionmentioning

confidence: 99%

Transcriptome Analysis Reveals Hub Genes Regulating Autophagy in Patients With Severe COVID-19

et al. 2022

View full text Add to dashboard Cite

show abstract

“…The signal transducing adapter molecule (STAM) gene forms the endosomal sorting complex required for transport-0 (ESCRT-0), and vacuolar protein sorting-associated protein 37B (VPS37B) is a component of the ESCRT-I complex. All the four genes are components of the ESCRT complex, and several viruses take advantage of the ESCRT system for proliferation, budding, and transmission in infected cells (Ju et al, 2021;Meng et al, 2021). Consequently, we supposed the infection of SARS-CoV-2 may be allied to the ESCRT system.…”

Section: Discussionmentioning

confidence: 99%