<scp>LFR</scp> is functionally associated with <scp>AS</scp>2 to mediate leaf development in Arabidopsis

Lin, Xiaowei; Gu, Dandan; Zhao, Hongtao; Peng, Yue; Zhang, Guofang; Yuan, Tingting; Li, Mengge; Wang, Zhijuan; Wang, Xiutang

doi:10.1111/tpj.13973

Cited by 14 publications

(33 citation statements)

References 63 publications

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“…The Leaf and Flower-Related gene ( LFR ) encodes a nuclear protein with the Armadillo (ARM)-repeat domains ( Wang et al, 2009 ), which are involved in protein–protein interactions ( Samuel et al, 2006 ). Arabidopsis with a loss-of-function mutation in the LFR gene exhibit pleiotropic phenotypes during leaf and flower development ( Wang et al, 2009 , 2012 ; Lin et al, 2018 ). LFR has been isolated from tandem affinity-purified protein complexes using SWIP37B ( Vercruyssen et al, 2014 ).…”

Section: Introductionmentioning

confidence: 99%

LFR Physically and Genetically Interacts With SWI/SNF Component SWI3B to Regulate Leaf Blade Development in Arabidopsis

et al. 2021

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Leaves start to develop at the peripheral zone of the shoot apical meristem. Thereafter, symmetric and flattened leaf laminae are formed. These events are simultaneously regulated by auxin, transcription factors, and epigenetic regulatory factors. However, the relationships among these factors are not well known. In this study, we conducted protein-protein interaction assays to show that our previously reported Leaf and Flower Related (LFR) physically interacted with SWI3B, a component of the ATP-dependent chromatin remodeling SWI/SNF complex in Arabidopsis. The results of truncated analysis and transgenic complementation showed that the N-terminal domain (25–60 amino acids) of LFR was necessary for its interaction with SWI3B and was crucial for LFR functions in Arabidopsis leaf development. Genetic results showed that the artificial microRNA knockdown lines of SWI3B (SWI3B-amic) had a similar upward-curling leaf phenotype with that of LFR loss-of-function mutants. ChIP-qPCR assay was conducted to show that LFR and SWI3B co-targeted the promoters of YABBY1/FILAMENTOUS FLOWER (YAB1/FIL) and IAA carboxyl methyltransferase 1 (IAMT1), which were misexpressed in lfr and SWI3B-amic mutants. In addition, the association between LFR and the FIL and IAMT1 loci was partly hampered by the knockdown of SWI3B. These data suggest that LFR interacts with the chromatin-remodeling complex component, SWI3B, and influences the transcriptional expression of the important transcription factor, FIL, and the auxin metabolism enzyme, IAMT1, in flattened leaf lamina development.

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Section: Introductionmentioning

confidence: 99%

LFR Physically and Genetically Interacts With SWI/SNF Component SWI3B to Regulate Leaf Blade Development in Arabidopsis

et al. 2021

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“…Moreover, as a putative CRC component, OsLFR might be recruited to target genes by specific transcription factors (TFs), and some TFs might be the targets of OsLFR. For example, in Arabidopsis, the LFR‐containing SWI/SNF CRC interacts with TF AS2 to ensure normal leaf morphology (Lin et al ., 2018). SWI2/SNF2 ATPases SYD and BRM interact with TFs LEAFY (LFY) and SEPALLATA 3 (SEP3) to ensure the correct pattern of flower organ formation (Wu et al ., 2012).…”

Section: Discussionmentioning

confidence: 99%

“…Subsequently, LFR was proven to be an essential regulator of a genetic network that modulates anther development (Wang et al ., 2012b). Moreover, LFR and the SWI/SNF CRC members were identified as ASYMMETRIC LEAVES 2 (AS2)‐binding partners to repress BREVIPEDICELLUS ( BP ) by modulating nucleosome occupancy during leaf development (Lin et al ., 2018). LFR interacts with several SWI/SNF CRC members (Yuan, 2012; Lin et al ., 2018; McWhite et al ., 2020).…”

Section: Introductionmentioning

confidence: 99%

“…Moreover, LFR and the SWI/SNF CRC members were identified as ASYMMETRIC LEAVES 2 (AS2)‐binding partners to repress BREVIPEDICELLUS ( BP ) by modulating nucleosome occupancy during leaf development (Lin et al ., 2018). LFR interacts with several SWI/SNF CRC members (Yuan, 2012; Lin et al ., 2018; McWhite et al ., 2020). In addition, LFR could be obtained through tandem affinity‐purified SWI/SNF complex protein (Vercruyssen et al ., 2014).…”

Section: Introductionmentioning

confidence: 99%

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OsLFR is essential for early endosperm and embryo development by interacting with SWI/SNF complex members in Oryza sativa

Wen

Meng

et al. 2020

The Plant Journal

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Rice (Oryza sativa L.) endosperm provides the developing embryo with nutrients and provides human beings with a staple food. The embryo eventually develops into a new sporophyte generation. Despite their important roles, the molecular mechanisms underlying early-stage endosperm and embryo development remain elusive. Here, we established the fundamental functions of rice OsLFR, an ortholog of the Arabidopsis SWI/SNF chromatin-remodeling complex (CRC) component LFR. OsLFR was expressed primarily in the rice spikelets and seeds, and the OsLFR protein was localized to the nucleus. We conducted genetic, cellular and molecular analyses of loss-of-function mutants and transgenic rescue lines. OsLFR depletion resulted in homozygous lethality in the early seed stage through endosperm and embryo defects, which could be successfully recovered by the OsLFR genomic sequence. Cytological observations revealed that the oslfr endosperm had relatively fewer free nuclei, had abnormal and arrested cellularization, and demonstrated premature programed cell death: the embryo was reduced in size and failed to differentiate. Transcriptome profiling showed that many genes, involved in DNA replication, cell cycle, cell wall assembly and cell death, were differentially expressed in a knockout mutant of OsLFR (oslfr-1), which was consistent with the observed seed defects. Protein-protein interaction analysis showed that OsLFR physically interacts with several putative rice SWI/SNF CRC components. Our findings demonstrate that OsLFR, possibly as one component of the SWI/SNF CRC, is an essential regulator of rice seed development, and provide further insights into the regulatory mechanism of early-stage rice endosperm and embryo development.

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“…AS2–AS1 interacts with the BP promoter, likely through the recruitment of the chromatin-remodeling factor HIRA (histone-regulator A) and forms a repressive chromatin state [ 19 ]. AS2–AS1 also interacts with LEAF FLOWER RELATED (LFR) in the chromatin remodeling complex and is associated with H3K27me3 in the BP gene, but not with the ETT/ARF3 gene [ 119 ]. AS2–AS1 is required for the correct temporal repression of ETT/ARF3 , which involves a PRC2-independent mechanism [ 50 ].…”

Section: As2–as1 Binds To Exon 1 Of the Target Gene Ett/mentioning

confidence: 99%

Roles of ASYMMETRIC LEAVES2 (AS2) and Nucleolar Proteins in the Adaxial–Abaxial Polarity Specification at the Perinucleolar Region in Arabidopsis

Iwakawa

Takahashi

Machida

et al. 2020

IJMS

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Leaves of Arabidopsis develop from a shoot apical meristem grow along three (proximal–distal, adaxial–abaxial, and medial–lateral) axes and form a flat symmetric architecture. ASYMMETRIC LEAVES2 (AS2), a key regulator for leaf adaxial–abaxial partitioning, encodes a plant-specific nuclear protein and directly represses the abaxial-determining gene ETTIN/AUXIN RESPONSE FACTOR3 (ETT/ARF3). How AS2 could act as a critical regulator, however, has yet to be demonstrated, although it might play an epigenetic role. Here, we summarize the current understandings of the genetic, molecular, and cellular functions of AS2. A characteristic genetic feature of AS2 is the presence of a number of (about 60) modifier genes, mutations of which enhance the leaf abnormalities of as2. Although genes for proteins that are involved in diverse cellular processes are known as modifiers, it has recently become clear that many modifier proteins, such as NUCLEOLIN1 (NUC1) and RNA HELICASE10 (RH10), are localized in the nucleolus. Some modifiers including ribosomal proteins are also members of the small subunit processome (SSUP). In addition, AS2 forms perinucleolar bodies partially colocalizing with chromocenters that include the condensed inactive 45S ribosomal RNA genes. AS2 participates in maintaining CpG methylation in specific exons of ETT/ARF3. NUC1 and RH10 genes are also involved in maintaining the CpG methylation levels and repressing ETT/ARF3 transcript levels. AS2 and nucleolus-localizing modifiers might cooperatively repress ETT/ARF3 to develop symmetric flat leaves. These results raise the possibility of a nucleolus-related epigenetic repression system operating for developmental genes unique to plants and predict that AS2 could be a molecule with novel functions that cannot be explained by the conventional concept of transcription factors.

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LFR is functionally associated with AS2 to mediate leaf development in Arabidopsis

Cited by 14 publications

References 63 publications

LFR Physically and Genetically Interacts With SWI/SNF Component SWI3B to Regulate Leaf Blade Development in Arabidopsis

LFR Physically and Genetically Interacts With SWI/SNF Component SWI3B to Regulate Leaf Blade Development in Arabidopsis

OsLFR is essential for early endosperm and embryo development by interacting with SWI/SNF complex members in Oryza sativa

Roles of ASYMMETRIC LEAVES2 (AS2) and Nucleolar Proteins in the Adaxial–Abaxial Polarity Specification at the Perinucleolar Region in Arabidopsis

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