<scp>NONO</scp> regulates B‐cell development and B‐cell receptor signaling

Zhang, Yongguang; Cui, Dongya; Huang, Miaohui; Zheng, Yongwei; Zheng, Baijiao; Chen, Liling; Chen, Qi

doi:10.1096/fj.202201909rr

Cited by 3 publications

(2 citation statements)

References 60 publications

(132 reference statements)

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“…Some RBPs, such as DDX3X, SFPQ, SRRM1, UPF2, etc. play an important role in B-cell-related diseases (Supplementary Figure S2A) (34)(35)(36)(37).…”

Section: Scrna-seq Analysis Revealed Dysregulated Rbps and Their Regu...mentioning

confidence: 99%

Single-cell transcriptome revealed dysregulated RNA-binding protein expression patterns and functions in human ankylosing spondylitis

Ren,

Li,

Wang

et al. 2024

Front. Med.

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ObjectiveTo explore the expression characteristics and regulatory patterns of RBPs in different immune cell types of AS, and to clarify the potential key role of RBPs in the occurrence and development of AS disease.MethodsPBMC sample data from scRNA-seq (HC*29, AS*10) and bulk RNA-seq (NC*3, AS*5) were selected for correlation analysis.Results(1) Compared with the HC group, the numbers of B, DC (dendritic cells), CD14+ Mono and CD8+ T cells were increased in AS group, while the numbers of platelet (platelets), CD8+ NKT, CD16+ Mono (non-classical monocytes), Native CD4+ T and NK were decreased. (2) Through the analysis of RBP genes in B cells, some RBPs were found to play an important role in B cell differentiation and function, such as DDX3X, SFPQ, SRRM1, UPF2. (3) It may be related to B-cell receptor, IgA immunity, NOD-like receptor and other signaling pathways; Through the analysis of RBP genes in CD8+ T cells, some RBPs that play an important role in the immune regulation of CD8+ T were found, such as EIF2S3, EIF4B, HSPA5, MSL3, PABPC1 and SRSF7; It may be related to T cell receptor, TNF, IL17 and other signaling pathways. (4) Based on bulk RNA-seq, it was found that compared with HC and AS patients, differentially expressed variable splicing genes (RASGs) may play an important role in the occurrence and development of AS by participating in transcriptional regulation, protein phosphorylation and ubiquitination, DNA replication, angiogenesis, intracellular signal transduction and other related pathways.ConclusionRBPs has specific expression characteristics in different immune cell types of AS patients, and has important regulatory functions. Its abnormal expression and regulation may be closely related to the occurrence and development of AS.

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“…Some RBPs, such as DDX3X, SFPQ, SRRM1, UPF2, etc. play an important role in B-cell-related diseases (Supplementary Figure S2A) (34)(35)(36)(37).…”

Section: Scrna-seq Analysis Revealed Dysregulated Rbps and Their Regu...mentioning

confidence: 99%

Single-cell transcriptome revealed dysregulated RNA-binding protein expression patterns and functions in human ankylosing spondylitis

Ren,

Li,

Wang

et al. 2024

Front. Med.

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“…TD and TI antigen immunizations were performed as described. 29 8-to 10-week-old mice were immunized intraperitoneally with 100 μg of TD antigen nitrophenyl-keyhole limpet hemocyanin (NP-KLH; Biosearch) in alum (Thermo Fisher) or 50 μg TI antigen trinitrophenyl-Ficoll (TNP-Ficoll; Biosearch) in alum. Sera were collected before and at 7, 14, 21, and 28 days after immunization.…”

Section: T-cell-dependent (Td) and T-cell-independent (Ti) Antigen Im...mentioning

confidence: 99%

CD30 plays a role in T‐dependent immune response and T cell proliferation

Cui,

Zhang,

Chen

et al. 2023

The FASEB Journal

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CD30 is a member of the tumor necrosis factor receptor (TNFR) superfamily and expressed in both normal and malignant lymphoid cells. However, the role of CD30 in lymphopoiesis is not known. In this study, we showed CD30 was expressed both in T and B cells, but its deficiency in mice had no effect on T‐ and B‐cell development. In fact, CD30 deficiency attenuated B‐cell response to T‐cell‐dependent antigens. The impaired B cell response in CD30‐deficient mice is caused by the reduction of activation‐induced cytidine deaminase (AID) expression. Moreover, CD30‐deficient mice exhibited decreased TCR‐mediated T cell proliferation and slightly impaired TCR signaling. High‐throughput RNA sequencing analysis revealed that CD30 deficiency led to a decrease of FOXO‐autophagy axis in T cells upon TCR stimulation. Thus, CD30 positively regulates T‐cell‐dependent immune response and T cell proliferation.

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