Screen-Printed All-Polymer Aptasensor for Impedance Based Detection of Influenza A Virus

Kirkegaard, Julie; Rozlosnik, Noémi

doi:10.1007/978-1-4939-6911-1_5

Cited by 11 publications

(11 citation statements)

References 10 publications

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“…Detection took under 30 min, and the minimum detection limitation was 0.0128 HAU. Following the same principle, aptasensors were developed to detect influenza A virus (Kirkegaard and Rozlosnik, 2017) and vaccinia virus (Labib et al, 2012). Karash et al (2016) designed an analogous aptasensor to detect H5N1 in chicken tracheal samples.…”

Section: Applications Of Aptamers In Virus Detectionmentioning

confidence: 99%

Application of Aptamers in Virus Detection and Antiviral Therapy

et al. 2019

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Viral infections can cause serious diseases for humans and animals. Accurate and early detection of viruses is often crucial for clinical diagnosis and therapy. Aptamers are mostly single-stranded nucleotide sequences that are artificially synthesized by an in vitro technology known as the Systematic Evolution of Ligands by Exponential Enrichment (SELEX). Similar to antibodies, aptamers bind specifically to their targets. However, compared with antibody, aptamers are easy to synthesize and modify and can bind to a broad range of targets. Thus, aptamers are promising for detecting viruses and treating viral infections. In this review, we briefly introduce aptamer-based biosensors (aptasensors) and describe their applications in rapid detection of viruses and as antiviral agents in treating infections. We summarize available data about the use of aptamers to detect and inhibit viruses. Furthermore, for the first time, we list aptamers specific to different viruses that have been screened out but have not yet been used for detecting viruses or treating viral infections. Finally, we analyze barriers and developing perspectives in the application of aptamer-based virus detection and therapeutics.

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Section: Applications Of Aptamers In Virus Detectionmentioning

confidence: 99%

Application of Aptamers in Virus Detection and Antiviral Therapy

et al. 2019

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“…The influenza virus variants detection was at 1.0 nM concentration level. Kirkegaard et al [135] invented screen-printed aptasensor (PEDOT) for impedance-based influenza A detection. Ozcelik et al [136] evaluated the optofluidic wavelength division multiplexing method for single-virus detection.…”

Section: Novel Detection Methods Of Influenza Virusmentioning

confidence: 99%

Detection Methods of Human and Animal Influenza Virus—Current Trends

Dziąbowska¹,

Czaczyk²,

Nidzworski³

2018

Biosensors

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The basic affairs connected to the influenza virus were reviewed in the article, highlighting the newest trends in its diagnostic methods. Awareness of the threat of influenza arises from its ability to spread and cause a pandemic. The undiagnosed and untreated viral infection can have a fatal effect on humans. Thus, the early detection seems pivotal for an accurate treatment, when vaccines and other contemporary prevention methods are not faultless. Public health is being attacked with influenza containing new genes from a genetic assortment between animals and humankind. Unfortunately, the population does not have immunity for mutant genes and is attacked in every viral outbreak season. For these reasons, fast and accurate devices are in high demand. As currently used methods like Rapid Influenza Diagnostic Tests lack specificity, time and cost-savings, new methods are being developed. In the article, various novel detection methods, such as electrical and optical were compared. Different viral elements used as detection targets and analysis parameters, such as sensitivity and specificity, were presented and discussed.

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“…Therefore, aptamers have been identified to bind with affinity to HA antigens from different strains of influenza. For further applications in diagnosis and possibly therapy, aptamers can be modified with biotin [ 52 , 53 ], amine groups [ 54 ], fluorophores [ 55 , 56 , 57 , 58 ], and thiol groups that will be attached to nanostructured materials, such as polymers [ 59 , 60 ], gold nanoparticles [ 61 , 62 , 63 ], metallic semiconductors [ 64 , 65 ], carbon-nanotubes [ 66 , 67 ], nanocomposites [ 68 ], and microspheres [ 69 ].…”

Section: Aptamers Targeting Influenza Virusmentioning

confidence: 99%

Aptamer Applications in Emerging Viral Diseases

et al. 2021

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Aptamers are single-stranded DNA or RNA molecules which are submitted to a process denominated SELEX. SELEX uses reiterative screening of a random oligonucleotide library to identify high-affinity binders to a chosen target, which may be a peptide, protein, or entire cells or viral particles. Aptamers can rival antibodies in target recognition, and benefit from their non-proteic nature, ease of modification, increased stability, and pharmacokinetic properties. This turns them into ideal candidates for diagnostic as well as therapeutic applications. Here, we review the recent accomplishments in the development of aptamers targeting emerging viral diseases, with emphasis on recent findings of aptamers binding to coronaviruses. We focus on aptamer development for diagnosis, including biosensors, in addition to aptamer modifications for stabilization in body fluids and tissue penetration. Such aptamers are aimed at in vivo diagnosis and treatment, such as quantification of viral load and blocking host cell invasion, virus assembly, or replication, respectively. Although there are currently no in vivo applications of aptamers in combating viral diseases, such strategies are promising for therapy development in the future.

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Screen-Printed All-Polymer Aptasensor for Impedance Based Detection of Influenza A Virus

Cited by 11 publications

References 10 publications

Application of Aptamers in Virus Detection and Antiviral Therapy

Application of Aptamers in Virus Detection and Antiviral Therapy

Detection Methods of Human and Animal Influenza Virus—Current Trends

Aptamer Applications in Emerging Viral Diseases

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