Screen-printed electrodes modified with carbon black and polyelectrolyte films for determination of cancer marker carbohydrate antigen 19-9

Ibáñez-Redín, Gisela; Materón, Elsa M.; Furuta, Roberto H. M.; Wilson, Deivy; Nascimento, Gustavo Freitas do; Melendez, Matias Eliseo; Carvalho, André Lopes; Reis, Rui Manuel; Oliveira, Osvaldo N.; Gonçalves, Débora

doi:10.1007/s00604-020-04404-6

Cited by 33 publications

(16 citation statements)

References 42 publications

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“…The corresponding calibration equation is ∆I (µA) = 1.58 + 5.39 log C (U•mL −1 ), having the regression coefficient (R 2 ) of 0.9974 and the limit of detection of 0.82 U•mL −1 based on a signal-to-noise ratio of 3 (S/N = 3). To better further evaluate the electrochemical immunosensors, the performance of our Zn-Co-S@G nanocomposite electrochemical immunosensor is compared with that of other electrochemical immunosensors for detecting the biomarker CA19-9, according to previous reports, which was better or comparable to that of the previous reports, as shown in Table 1 [1,6,[26][27][28][29].…”

Section: Resultsmentioning

confidence: 81%

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Construction of a Label-Free Electrochemical Immunosensor Based on Zn-Co-S/Graphene Nanocomposites for Carbohydrate Antigen 19-9 Detection

Tian

et al. 2021

Nanomaterials

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Nanocomposites of the binary transition metal sulfide Zn-Co-S/graphene (Zn-Co-S@G) were synthesized through a one-step hydrothermal method. They may be useful in the construction of an electrochemical immunosensor for carbohydrate antigen 19-9 (CA19-9) detection. Zn-Co-S dot-like nanoparticles uniformly covered the surface of graphene to form an interconnected conductive network, ensuring strong interaction between transition metal sulfide and graphene, which can expose numerous electroactive sites leading to the improvement of the amplified electrochemical signal toward a direct reduction of H2O2. Thus, the construction of an electrochemical immunosensor using Zn-Co-S@G nanocomposites showed outstanding sensing properties for detecting CA19-9. The constructed electrochemical immunosensor exhibited a good linear relationship in the range of 6.3 U·mL−1–300 U·mL−1, with the limit of detection at 0.82 U·mL−1, which makes it a promising candidate for an electrochemical immunosensor.

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Section: Resultsmentioning

confidence: 81%

“…(b) The linear range of BSA/anti-CA19-9/GA/CHIT/Zn-Co-S@G/GCE in Figure 8a. Polythionine-Au 0.26 6.5-520 [6] Polythionine/sodium dodecyl sulphate 0.45 5-400 [26] AuPt nanocalliandras 0.03 0.05-50 [27] Carbon nanoonions/grphene oxide 0.12 0.3-100 [28] Carbon black/polyelectrolytes 0.07 0.01-40 [29] Zn-Co-S/graphene 0.82 6.3-300 This work…”

Section: Resultsmentioning

confidence: 99%

Construction of a Label-Free Electrochemical Immunosensor Based on Zn-Co-S/Graphene Nanocomposites for Carbohydrate Antigen 19-9 Detection

Tian

et al. 2021

Nanomaterials

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“…108 As 6 1,0 × 10 −3 mol L -1 em tampão fosfato 0,1 mol L −1 pH 7. Parâmetros dos voltamogramas de pulso diferencial: tempo de modulação = 0,05 s, amplitude de modulação = 0,09 V e incremento de potencial = 0,05 V. Fonte: Adaptada de IBÁÑEZ-REDÍN et al 101 Posteriormente foi realizada a deposição dos filmes de CB-PEI e CB-PAA nas superfícies dos eletrodos de trabalho pré-tratados. Tanto a concentração de CB nos compósitos quanto o tempo de adsorção dos filmes foram otimizados considerando como critérios a obtenção de voltamogramas de pulso diferencial com boa definição e elevado sinal analítico.…”

Section: Deposicão Lbl Dos Filmes De Cb-polieletrólitos Nos Spcesunclassified

“…Experimentos realizados em solução K 3 Fe(CN) 6 /K 4 Fe(CN) 6 1,0×10 −3 mol L −1 em tampão fosfato 0,1 mol L −1 pH 7. Parâmetros: tempo de modulação = 0,05 s, amplitude de modulação = 0,09 V e incremento de potencial = 0,05 V. Fonte: Adaptada de IBÁÑEZ-REDÍN et al 101 Na Figura 2.9 é apresentada uma proposta para o perfil de difusão da sonda redox Fe(CN) 6 3− /Fe(CN) 6 4− através dos filmes de CB-polieletrólito baseado em BARREIRA e colaboradores. 109 Vale ressaltar que a resposta eletroquímica de eletrodos modificados com compósitos CB-polieletrólito depende especificamente do tipo de sonda utilizada e do seu mecanismo de transferência eletrônica.…”

Section: Deposicão Lbl Dos Filmes De Cb-polieletrólitos Nos Spcesunclassified

“…64 Figura 2.11 -Imagens de SEM obtidas para SPCE-pré-tratados (A-B) e SPCE/(CB-PEI/CB-PAA) 2 (C-D) em diferentes ampliações. Fonte: Adaptada de IBÁÑEZ-REDÍN et al 101 A morfologia dos eletrodos foi também estudada utilizando-se AFM (Figura 2.12 (A-B)). A rugosidade média calculada a partir das micrografias para uma ampliação de 1,1 µm foi de (38,7±0,6) nm e (78,7±0,9) nm para SPCE e SPCE/(CB-PEI/CB-PAA) 2 , respectivamente.…”

Section: Deposicão Lbl Dos Filmes De Cb-polieletrólitos Nos Spcesunclassified

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Novas estratégias de fabricação de imunossensores sem marcação à base de eletrodos impressos de carbono para a detecção de biomarcadores de câncer

Redin¹

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Label-free electrochemical immunosensors based on screen-printed carbon electrodes are a promising tool for performing fast, simple and economical detection of cancer biomarkers.However, despite the potential of these devices, some limitations, such as their low sensitivity, need to be overcome before their widespread use as technology for clinical analysis. Herein, we proposed different strategies to modify screen-printed carbon electrodes, aiming to improve the analytical signal and the surface area for the immobilization of antibodies, which are two essential parameters for reaching good analytical performance in label-free immunosensors. We explored the application potential of carbon black and NiFe 2 O 4 nanoparticles, two low-cost nanomaterials with excellent electrochemical properties, which remain relatively unexplored in the construction of electrochemical immunosensors. In a first approach, an immunosensor was developed for detecting the carbohydrate antigen CA 19-9 using electrodes modified with composites of carbon black and the polyelectrolytes polyethyleneimine and polyacrylic acid. The immunosensor exhibited a low detection limit of 0.07 U mL − 1 , in addition to an excellent fabrication reproducibility, stability and selectivity.The proposed device showed good correlation with commercial immunoassays in the determination of CA 19-9 in cell lysates and human serum samples from pancreatic cancer patients. An immunosensor for detecting the tumor protein p53 was also developed, using electrodes modified with films of polyethyleneimine and NiFe 2 O 4 nanoparticles. The analytical parameters of the devices are among the best reported in the literature for determination of p53, with a low detection limit of 5 fg mL −1 . Also, the immunosensors showed an excellent selectivity toward p53 detection in fetal bovine serum, saliva and cell lysate samples. The platforms proposed herein combined the numerous advantages of screenprinted electrodes with the excellent properties of the studied nanomaterials, allowing the development of low-cost (˂BRL$ 4/ test) and disposable devices with excellent analytical performance, which can be adapted for the detection of several disease markers.

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Recent Progress of Layer‐by‐layer Assembly, Free‐Standing Film and Hydrogel Based on Polyelectrolytes

Ivanov

Pershina

Nikolaev

et al. 2021

Macromolecular Bioscience

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Nowadays, polyelectrolytes play an essential role in the development of new materials. Their use allows creating new properties of materials and surfaces and vary them in a wide range. Basically, modern methods are divided into three areas—the process of layer‐by‐layer deposition, free‐standing films, and hydrogels based on polyelectrolytes. Layer‐by‐layer assembly of polyelectrolytes on various surfaces is a powerful technique. It allows giving surfaces new properties, for example, protect them from corrosion. Free‐standing films are essential tools for the design of membranes and sensors. Hydrogels based on polyelectrolytes have recently shown their applicability in electrical and materials science. The creation of new materials and components with controlled properties can be achieved using polyelectrolytes. This review focuses on new technologies that have been developed with polyelectrolytes over the last five years.

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Screen-printed electrodes modified with carbon black and polyelectrolyte films for determination of cancer marker carbohydrate antigen 19-9

Cited by 33 publications

References 42 publications

Construction of a Label-Free Electrochemical Immunosensor Based on Zn-Co-S/Graphene Nanocomposites for Carbohydrate Antigen 19-9 Detection

Construction of a Label-Free Electrochemical Immunosensor Based on Zn-Co-S/Graphene Nanocomposites for Carbohydrate Antigen 19-9 Detection

Novas estratégias de fabricação de imunossensores sem marcação à base de eletrodos impressos de carbono para a detecção de biomarcadores de câncer

Recent Progress of Layer‐by‐layer Assembly, Free‐Standing Film and Hydrogel Based on Polyelectrolytes

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