Screening and Characterization of Potentially Suppressive Soils against Gaeumannomyces graminis under Extensive Wheat Cropping by Chilean Indigenous Communities

Durán, Paola; Jorquera, Milko A.; Viscardi, Sharon; Carrión, Víctor J.; Mora, Marı́a de la Luz; Pozo, Marı́a J.

doi:10.3389/fmicb.2017.01552

Cited by 44 publications

(55 citation statements)

References 50 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In a previous study from our group, we characterized several suppressive soils and evidenced the essential role of soil microbial communities in the Ggt suppression by six soils managed under ancestral agronomic practices as monoculture for more than 10 years ( Durán et al, 2017 ). However, there is little known regarding the structure of the rhizosphere microbial communities and pathogen abundance required to fully understand the role of different microbial groups in the control of Ggt in suppressive soils.…”

Section: Introductionmentioning

confidence: 97%

Microbial Community Composition in Take-All Suppressive Soils

et al. 2018

Self Cite

View full text Add to dashboard Cite

Gaeumannomyces graminis var. tritici (Ggt) is the main soilborne factor that affects wheat production around the world. Recently we reported the occurrence of six suppressive soils in monoculture areas from indigenous “Mapuche” communities, and evidenced that the suppression relied on the biotic component of those soils. Here, we compare the rhizosphere and endosphere microbial community structure (total bacteria, actinomycetes, total fungi, and ascomycetes) of wheat plants grown in suppressive and conducive soils. Our results suggested that Ggt suppression could be mediated mostly by bacterial endophytes, rather than rhizosphere microorganisms, since the community structure was similar in all suppressive soils as compared with conducive. Interestingly, we found that despite the lower incidence of take-all disease in suppressive soils, the Ggt concentration in roots was not significantly reduced in all suppressive soils compared to those growing in conducive soil. Therefore, the disease suppression is not always related to a reduction of the pathogen biomass. Furthermore, we isolated endophytic bacteria from wheat roots growing in suppressive soils. Among them we identified Serratia spp. and Enterobacter spp. able to inhibit Ggt growth in vitro. Since the disease, but not always pathogen amount, was reduced in the suppressive soils, we propose that take all disease suppressiveness is not only related to direct antagonism to the pathogen.

show abstract

Section: Introductionmentioning

confidence: 97%

Microbial Community Composition in Take-All Suppressive Soils

et al. 2018

Self Cite

View full text Add to dashboard Cite

show abstract

“…Suppressiveness of G. graminis var. tritici in certain soils is produced by the action of the different members of the microbial biomass present in these soils (Yang et al, 2011;Durán et al, 2017;Yin et al, 2017), which could be reduced by soil sterilization conducted prior to establishing the experiments. Although the specific suppression by strains that produce 2,4-DAPG tends to be higher than the rest of the microbial rhizosphere biomass, variability in the control of Ggt by beneficial bacteria is a normal phenomenon (Kwak et al, 2012).…”

Section: Discussionmentioning

confidence: 99%

“…Take-all disease, caused by the ascomycete fungus G. graminis var. tritici (Ggt), causes severe losses in wheat (Triticum aestivum L.; Poaceae) crops in Chile (Andrade et al, 2011;Moya-Elizondo et al, 2015;Durán et al, 2017) and worldwide Yang et al, 2011;Zhang et al, 2017). This fungus triggers rotting and decomposition of the roots, crown, and basal culm of wheat plants and other cereals .…”

Section: Introductionmentioning

confidence: 99%

“…Bacteria from the genus Pseudomonas produce metabolites with antifungal effects such as 2,4-diacetylphloroglucinol (2,4-DAPG), hydrocyanic acid, pyoluteorin, pyrrolnitrin, and phenazine derivatives (Ramette et al, 2011;Kwak et al, 2012;Yang et al, 2017). Recently, the presence of 2,4-DAPG producing Pseudomonas bacteria has been reported in fields in southern Chile (Moya-Elizondo et al, 2013;Durán et al, 2017). Bacteria that produce 2,4-DAPG are a promising source for the development of seed inoculants for disease control, although this compound has high instability in the rhizosphere (Kwak et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Effect of saponins of Quillaja saponaria extracts in combination with Pseudomonas protegens to control Gaeumannomyces graminis var. tritici in wheat

González-Castillo¹,

Quezada-D'Angelo²,

Silva-Aguayo³

et al. 2018

Chil. j. agric. res.

View full text Add to dashboard Cite

Extracts rich in saponins from Quillaja saponaria Mol. and populations of rhizobacteria from the genus Pseudomonas, which produce antimicrobial compounds, have been associated with reduction of the fungus Gaeumannomyces graminis var. tritici, the causal agent of take-all disease, which is responsible for severe loss of wheat (Triticum aestivum L.) crops worldwide. However, there is a limited background on the interaction between these bacteria and natural triterpenoids. The aim of this research was to assess the effect of saponin rich extract on Pseudomonas protegens strains 2,4-diacetylphloroglucinol-producers under in vitro and in plant conditions and determining the synergistic effect to be used together to control G. graminis var. tritici in wheat plants. We determined that 8% and 90% of saponins rich Q. saponaria extracts have a differential effect on P. protegens according to their purity (P ≤ 0.05). On wheat seedlings, quillaja extract with 90% of saponins did not affect the three antagonistic bacterial strain populations assessed, but affected biofilm formation at saponins concentration of 7360 mg L-1. Pseudomonas protegens strains had a variable antagonist activity in wheat plants, and controlled the fungus when were combined with different concentrations of pure Q. saponaria extract, with the concentration of 1840 ppm reducing the take-all disease in 32.5% with respect to the control inoculated with G. graminis var. tritici (P ≤ 0.05). However, no synergistic effects when the plant extract was combined with the bacterial strains were observed. These results showed the promising and complexity of combining bacterial and plant extract to develop a biopesticide, which could control this fungal disease.

show abstract

“…Plants in the Brassicaceae Burnett are primarily non-mycorrhizal (Fitter 2005), but their roots harbor complex communities of endophytic fungi that include a great abundance and diversity of helotialean species, many of which are phylogenetically close to Cadophora (Almario et al 2017;Glynou et al 2018a;Thiergart et al 2019;Maciá-Vicente et al 2020). Often, these Cadophora-like endophytes do not form evident parasitic interactions with the host, as appears to be common in endophytes from other lineages (Durán et al 2017;Kia et al 2017Kia et al , 2018Kia et al , 2019. Glynou et al (2016) found a phylotype, identi ed as Cadophora sp.…”

Section: Introductionmentioning

confidence: 99%

Brassicaceous roots as an unexpected diversity hot-spot of helotialean endophytes

Maciá‐Vicente

Piepenbring

Koukol

2020

Preprint

View full text Add to dashboard Cite

A high number of fungal strains were isolated from roots of Brassicaceae species collected across western and southern Europe, resulting in an unexpectedly rich collection of Cadophora species. These isolates enable us to present a new and comprehensive view of the ecological, morphological, and phylogenetic traits of root-inhabiting members of this helotialean genus. We provide phylogenetic placement of all of our isolates based on a four-gene dataset, analyze their phenotypic traits in relation to their phylogenetic relationships, and infer the potential distribution ranges of the species by sequence comparisons with available databases. We consider seven well supported phylogenetic lineages as species new to science. Six further lineages probably also represent new species but remain undescribed due to the lack of diagnostic morphological characters. Our results show that Cadophora , as currently circumscribed, is paraphyletic and encompasses a broad spectrum of morphologies and lifestyles. Among the new species, only two ( C. ferruginea and C. constrictospora ) form phialides and conidia typical of Cadophora , three species ( C. echinata , C. gamsii and C. variabilis ) produce chains of swollen hyphal segments that may function as holoblastic conidia, and one species ( C. fascicularis ) produces chains of holoblastic ramoconidia and conidia. Ancestral state reconstruction analysis suggests that phialidic conidiogenesis evolved several times in Cadophora sensu lato from a putatively holoblastic common ancestor. Most Cadophora lineages are rare as estimated from the availability of sequence data, in spite of having relatively wide distribution ranges, whereas five lineages may represent endemic relationships given their restricted distributions. Our dataset, probably the most comprehensive available for Cadophora , nevertheless shows knowledge gaps concerning the phylogenetic relationships within this genus and highlights a need for further investigation.

show abstract

Screening and Characterization of Potentially Suppressive Soils against Gaeumannomyces graminis under Extensive Wheat Cropping by Chilean Indigenous Communities

Cited by 44 publications

References 50 publications

Microbial Community Composition in Take-All Suppressive Soils

Microbial Community Composition in Take-All Suppressive Soils

Effect of saponins of Quillaja saponaria extracts in combination with Pseudomonas protegens to control Gaeumannomyces graminis var. tritici in wheat

Brassicaceous roots as an unexpected diversity hot-spot of helotialean endophytes

Contact Info

Product

Resources

About