Screening and comparative metabolic profiling of high lipid content microalgae strains for application in wastewater treatment

Piligaev, A. V.; Sorokina, K. N.; Шашков, М. В.; Parmon, Valentin N.

doi:10.1016/j.biortech.2017.11.063

Cited by 64 publications

(26 citation statements)

References 37 publications

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“…The total UFA concentration in the biodiesels derived from OPRs 1 and 3 was 65.1 and 67.2%, respectively, which was approximately two times greater compared to SFAs (34.9 and 32.8%, respectively). The low PUFA content and high SFA and MUFA content are among the most important indicators of biodiesel quality ( Piligaev et al, 2018 ). The fatty acid profiles of microalgal oils generally affect the qualities of the biodiesel produced.…”

Section: Discussionmentioning

confidence: 99%

Assessment of biomass potentials of microalgal communities in open pond raceways using mass cultivation

et al. 2020

PeerJ

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Metagenome studies have provided us with insights into the complex interactions of microorganisms with their environments and hosts. Few studies have focused on microalgae-associated metagenomes, and no study has addressed aquatic microalgae and their bacterial communities in open pond raceways (OPRs). This study explored the possibility of using microalgal biomasses from OPRs for biodiesel and biofertilizer production. The fatty acid profiles of the biomasses and the physical and chemical properties of derived fuels were evaluated. In addition, the phenotype-based environmental adaptation ability of soybean plants was assessed. The growth rate, biomass, and lipid productivity of microalgae were also examined during mass cultivation from April to November 2017. Metagenomics analysis using MiSeq identified ∼127 eukaryotic phylotypes following mass cultivation with (OPR 1) or without (OPR 3) a semitransparent film. Of these, ∼80 phylotypes were found in both OPRs, while 23 and 24 phylotypes were identified in OPRs 1 and 3, respectively. The phylotypes belonged to various genera, such as Desmodesmus, Pseudopediastrum, Tetradesmus, and Chlorella, of which, the dominant microalgal species was Desmodesmus sp. On average, OPRs 1 and 3 produced ∼8.6 and 9.9 g m−2 d−1 (0.307 and 0.309 DW L−1) of total biomass, respectively, of which 14.0 and 13.3 wt% respectively, was lipid content. Fatty acid profiling revealed that total saturated fatty acids (mainly C16:0) of biodiesel obtained from the microalgal biomasses in OPRs 1 and 3 were 34.93% and 32.85%, respectively; total monounsaturated fatty acids (C16:1 and C18:1) were 32.40% and 31.64%, respectively; and polyunsaturated fatty acids (including C18:3) were 32.68% and 35.50%, respectively. Fuel properties determined by empirical equations were within the limits of biodiesel standards ASTM D6751 and EN 14214. Culture solutions with or without microalgal biomasses enhanced the environmental adaptation ability of soybean plants, increasing their seed production. Therefore, microalgal biomass produced through mass cultivation is excellent feedstock for producing high-quality biodiesel and biofertilizer.

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Section: Discussionmentioning

confidence: 99%

Assessment of biomass potentials of microalgal communities in open pond raceways using mass cultivation

et al. 2020

PeerJ

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“…The application 1 3 of such wastewater sources can lead to production of thousands of tons of microalgae biomass, which can be employed for biofuel production. Moreso, the ability of microalgae to utilize a myriad of contaminants ranging from organic carbon, inorganic (nitrates, phosphate, nitrites, sulfates, and ammonium), and metals present in wastewater sources is well reported [5]. The main motive of wastewater treatment is to reduce various contaminants that can adversely affect the aquatic life and thereby impact negatively on the environment [6].…”

Section: Introductionmentioning

confidence: 99%

“…in the wastewater supplemented growth medium Case 4, where the sulfate concentration was about 900 mg/L.Piligaev et al[5] attributed lower microalgae (Chlorella sorokiniana, Parachlorella kessleri, Chlorella protothecoidies, S. obliquus, Desmodesmus sp., Parachlorella sp., Micractinium sp., and Chlorella sp.) growth in municipal wastewater, compared to synthetic growth medium, to lower O. minuta concentrations of nitrate, phosphate, and the presence of other toxic contaminants in the wastewater.…”

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confidence: 99%

Assessment of Chlorella vulgaris, Scenedesmus obliquus, and Oocystis minuta for removal of sulfate, nitrate, and phosphate in wastewater

Ajala

Alexander

2020

Int J Energy Environ Eng

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Application of wastewater for algal biomass production can not only lead to production of thousands of tons of biomass for subsequent biofuel production, but also can provide for significant removal of contaminants in wastewater. The aim of the present study is to evaluate the growth, contaminant removal, and biochemical component (lipid, carbohydrate, and protein) accumulation potential of Chlorella vulgaris, Scenedesmus obliquus, and Oocystis minuta cells in wastewater supplemented with different concentrations of sulfate, nitrate, and phosphate. The results show maximum biomass productivity of 33, 19, and 98 mg dw/L/d for C. vulgaris, S. obliquus, and O. minuta, respectively. Phosphate removal (more than 90%) was highest in the culture with O. minuta; about 93% nitrate was removed by C. vulgaris, and the highest sulfate removal of 36% was observed in the culture with S. obliquus. The biochemical composition of the microalgae cells is in the ranges of 22-65% carbohydrate, 19-38% protein, and 8-17% lipid. This indicates that carbohydrate and protein are preferentially accumulated as compared to lipids under the growth conditions investigated for each of the microalgae strains.Keywords Remediation · Lipids · Microalgae · Carbohydrate · Photosynthetic organism Abbreviations ODOptical density AFDW Ash-free dry weight R 2 Coefficient of determination P Biomass productivity t 1 , t 2 Culture duration, time t 1 and t 2 C 1 , C 2 Biomass concentrations at time, t 1 and t 2 A Mass of lipid in the extract BMass of dried microalgae biomass BSA Bovine serum albumin * Sheriff Olalekan AjalaPublisher's Note Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

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“…MWW had the following composition [62]: COD 161.0 ± 5.0 mg L −1 , 27.7 ± 1.3 mg L −1 N-NH 4 , 0.2 ± 0.0 mg L −1 N-NO 3 , 1.8 ± 0.1 mg L −1 P-PO 4 , 10.7 ± 1.0 mg L −1 S-SO 4 , and 139.0 ± 5.0 mg L −1 Cl.…”

Section: Operational Stability Of Clea-estut1 During Hydrolysis Of Mamentioning

confidence: 99%

Preparation of Stable Cross-Linked Enzyme Aggregates (CLEAs) of a Ureibacillus thermosphaericus Esterase for Application in Malathion Removal from Wastewater

et al. 2018

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In this study, the active and stable cross-linked enzyme aggregates (CLEAs) of the thermostable esterase estUT1 of the bacterium Ureibacillus thermosphaericus were prepared for application in malathion removal from municipal wastewater. Co-expression of esterase with an E. coli chaperone team (KJE, ClpB, and ELS) increased the activity of the soluble enzyme fraction up to 200.7 ± 15.5 U mg−1. Response surface methodology (RSM) was used to optimize the preparation of the CLEA-estUT1 biocatalyst to maximize its activity and minimize enzyme loss. CLEA-estUT1 with the highest activity of 29.4 ± 0.5 U mg−1 (90.6 ± 2.7% of the recovered activity) was prepared with 65.1% (w/v) ammonium sulfate, 120.6 mM glutaraldehyde, and 0.2 mM bovine serum albumin at 5.1 h of cross-linking. The biocatalyst has maximal activity at 80 °С and pH 8.0. Analysis of the properties of CLEA-estUT1 and free enzyme at 50–80 °C and pH 5.0–10.0 showed higher stability of the biocatalyst. CLEA-estUT1 showed marked tolerance against a number of chemicals and high operational stability and activity in the reaction of malathion hydrolysis in wastewater (up to 99.5 ± 1.4%). After 25 cycles of malathion hydrolysis at 37 °С, it retained 55.2 ± 1.1% of the initial activity. The high stability and reusability of CLEA-estUT1 make it applicable for the degradation of insecticides.

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Screening and comparative metabolic profiling of high lipid content microalgae strains for application in wastewater treatment

Cited by 64 publications

References 37 publications

Assessment of biomass potentials of microalgal communities in open pond raceways using mass cultivation

Assessment of biomass potentials of microalgal communities in open pond raceways using mass cultivation

Assessment of Chlorella vulgaris, Scenedesmus obliquus, and Oocystis minuta for removal of sulfate, nitrate, and phosphate in wastewater

Preparation of Stable Cross-Linked Enzyme Aggregates (CLEAs) of a Ureibacillus thermosphaericus Esterase for Application in Malathion Removal from Wastewater

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