Screening and confirmatory GC-MS methods for the detection of trenbolone in bovine urine

Woźniak, Barbara; Matraszek-Żuchowska, Iwona; Semeniuk, Stanisław; Kłopot, Alicja; Żmudzki, J.

doi:10.2478/bvip-2013-0096

Cited by 4 publications

(4 citation statements)

References 14 publications

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“…Gas chromatography/mass spectrometry (GC/MS) has been extensively used to determine anabolic compounds in urine but a major drawback is the need of derivatization [8][9][10][11]. This task can be quite demanding for trenbolone since several derivatives are formed using its most usual derivatives agents due to epimerization of its keto group in position 3 unless quite specific conditions are employed [12,13].…”

Section: Introductionmentioning

confidence: 99%

Simultaneous determination of resorcylic acid lactones, β and α trenbolone and stilbenes in bovine urine by UHPLC/MS/MS

Fernández-Arauzo

Pimentel-Trapero

Hernández-Carrasquilla

2014

Journal of Chromatography B

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Section: Introductionmentioning

confidence: 99%

Simultaneous determination of resorcylic acid lactones, β and α trenbolone and stilbenes in bovine urine by UHPLC/MS/MS

Fernández-Arauzo

Pimentel-Trapero

Hernández-Carrasquilla

2014

Journal of Chromatography B

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“…Taking into account previous experience (Matraszek-Żuchowska et al 2012;Woźniak et al 2011Woźniak et al , 2013, in the presented study in order to choose the ions characteristic for the decomposition of the hormones, derivatives electron ionization was used, which is by far seen as the most common and standard form. EI allows getting satisfactory fragmentation of derivative, as well as the presence of the molecular ions [M +• ] (m/z) in the spectrum.…”

Section: Discussionmentioning

confidence: 99%

Determination of Hormones Residues in Milk by Gas Chromatography-Mass Spectrometry

2016

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T h e a rti c le p re s en t s th e u se of ga s chromatography-mass spectrometry (GC-MS) technique in a method for the determination of 18 anabolic hormones from synthetic stilbenes, steroids and resorcylic acid lactones (RALs) groups in raw milk and milk powder. Sample preparation consisted of liquid-liquid extraction with diethyl ether and purification by solid phase extraction (SPE). Prior to instrumental analysis, the reaction of derivatisation with the heptafluorobutyric anhydride or N-methyl-N-trimethylsilyltrifluoroacetamide was performed. Method validation was carried out according to the required performance criteria of the Commission Decision 2002/657/EC. The apparent recovery of all analytes at 1 μg L −1 (kg −1 ) level was ranged between 70.4 and 119.4 % with the coefficients of variation values less than 30 %. The decision limits (CCα) and the detection capabilities (CCβ) were in the range of from 0.11 to 0.44 μg L −1 (kg −1 ) and from 0.19 to 0.75 μg L −1 (kg −1 ), respectively. The procedure has been accredited and successfully applied as a screening method for the presence of hormone residues in the study of commercial samples of milk.

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“…In a study of residues, next to the properly selected detection techniques, it is very important to properly prepare the sample for analysis. Depending on the type of compound, either polar solvents such as methanol , acetonitrile , ethyl acetate , diethyl ether or non‐polar like petroleum ether or tert‐methyl butyl ether are used to extract the steroids from matrix. For further purification of the extract SPE with various sorbents was used, in particular C 18, NH 2 or SiOH .…”

Section: Introductionmentioning

confidence: 99%

Fast analysis of 19 anabolic steroids in bovine tissues by high performance liquid chromatography with tandem mass spectrometry

Woźniak

Matraszek-Żuchowska

Kłopot

et al. 2019

J of Separation Science

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For the detection of 19 steroid hormones in bovine muscle, a fast and sensitive liquid chromatography with electrospray ionization tandem mass spectrometry method was developed using both positive and negative ionization mode. Chromatographic separation on Poroshell 120‐EC C18 column was achieved in less than 10 min using isocratic elution of mobile phase of acetonitrile/methanol/water. The compounds were extracted from muscle tissue using ethyl acetate and quick, easy, cheap, effective, rugged, and safe technique. The purification of the obtained extract was performed by dispersive solid‐phase extraction with sorbents C18, primary secondary amine and magnesium sulphate. The method was validated in accordance with the Commission Decision 2002/657/EC. For all steroids tested good recoveries were obtained (from 51.2 to 121.4%) in the concentration range from decision limits until 5 µg/kg. The values of decision limits and the detection capabilities for individual compounds were in the range 0.10–0.48 and 0.17–0.95 µg/kg, respectively. The method was characterized by satisfactory linearity for most compounds (correlation coefficients > 0.99) and the reproducibility was lower than 35%. The elaborated procedure has met the criteria for confirmatory methods and is currently used in the official control of hormones.

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Screening and confirmatory GC-MS methods for the detection of trenbolone in bovine urine

Cited by 4 publications

References 14 publications

Simultaneous determination of resorcylic acid lactones, β and α trenbolone and stilbenes in bovine urine by UHPLC/MS/MS

Simultaneous determination of resorcylic acid lactones, β and α trenbolone and stilbenes in bovine urine by UHPLC/MS/MS

Determination of Hormones Residues in Milk by Gas Chromatography-Mass Spectrometry

Fast analysis of 19 anabolic steroids in bovine tissues by high performance liquid chromatography with tandem mass spectrometry

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