Screening and development of DNA aptamers as capture probes for colorimetric detection of patulin

Wu, Shijia; Duan, Nuo; Zhang, Weixiao; Zhao, Sen; Wang, Zhouping

doi:10.1016/j.ab.2016.05.024

Cited by 95 publications

(44 citation statements)

References 28 publications

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“…Initially, eight aptamer sequences were shortlisted, and an aptamer named PAT-11 was then selected as the final recognition element based on its low K d (dissociation constant) value, i.e., 21.83 nM, and the highest binding affinity and selectivity for PAT. The potential applicability of the selected aptamer sequence was further confirmed by developing an enzyme-chromogenic substrate system-based colorimetric aptasensor for the selective and sensitive detection of PAT in a real matrix [25]:…”

Section: Methodsmentioning

confidence: 99%

Development of an Impedimetric Aptasensor for Label Free Detection of Patulin in Apple Juice

et al. 2019

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In the present work, an aptasensing platform was developed for the detection of a carcinogenic mycotoxin termed patulin (PAT) using a label-free approach. The detection was mainly based on a specific interaction of an aptamer immobilized on carbon-based electrode. A long linear spacer of carboxy-amine polyethylene glycol chain (PEG) was chemically grafted on screen-printed carbon electrodes (SPCEs) via diazonium salt in the aptasensor design. The NH2-modified aptamer was then attached covalently to carboxylic acid groups of previously immobilized bifunctional PEG to build a diblock macromolecule. The immobilized diblocked molecules resulted in the formation of long tunnels on a carbon interface, while the aptamer was assumed as the gate of these tunnels. Upon target analyte binding, the gates were assumed to be closed due to conformational changes in the structure of the aptamer, increasing the resistance to the charge transfer. This increase in resistance was measured by electrochemical impedance spectroscopy, the main analytical technique for the quantitative detection of PAT. Encouragingly, a good linear range between 1 and 25 ng was obtained. The limit of detection and limit of quantification was 2.8 ng L−1 and 4.0 ng L−1, respectively. Selectivity of the aptasensor was confirmed with mycotoxins commonly occurring in food. The developed apta-assay was also applied to a real sample, i.e., fresh apple juice spiked with PAT, and toxin recovery up to 99% was observed. The results obtained validated the suitability and selectivity of the developed apta-assay for the identification and quantification of PAT in real food samples.

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Section: Methodsmentioning

confidence: 99%

Development of an Impedimetric Aptasensor for Label Free Detection of Patulin in Apple Juice

et al. 2019

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“…Several methods, such as atomic force microscopy [ 82 ], high-throughput sequencing [ 83 , 84 ], graphene oxide (GO) [ 85 , 86 ], cross-linking by UV [ 87 , 88 ], flow cytometry [ 89 , 90 ], and surface plasmon resonance (SPR) [ 91 , 92 ], have been fabricated in connection with SELEX for aptamer selection. These methods not only enrich the selection measures, but also promote the efficiency of aptamer selection.…”

Section: Aptamer Selectionmentioning

confidence: 99%

Aptamers used for biosensors and targeted therapy

Ning

2020

Biomedicine & Pharmacotherapy

101

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“…In fact, heavy metals can, like PAT, form strong covalent bonds with enzyme sulfhydryl groups. Recently, a PAT aptamer has been developed and defined by Wu, Duan, Zhang, Zhao, and Wang (2016). The oligonucleotide aptamer refers to a monocatenary DNA (or RNA) sequence.…”

Section: Current Application Of Biotechnology In the Face Of The Foodmentioning

confidence: 99%

“…This selected aptamer was later used as the selective component in a PAT detection method based on a chromogenic enzymatic substrate system. The results were very impressive; the colorimetric aptasensor gave a linear detection range of 5 × 10 −2 to 2.5 µg/L, and the detection limit was 4.8 × 10 −2 µg/L (Wu et al., 2016). To increase the transmission of signal of biosensors, lanthanide‐doped rare earth‐doped up‐conversion nanoparticles (UCNPs) have received considerable attention (Kwon et al., 2016).…”

Section: Current Application Of Biotechnology In the Face Of The Foodmentioning

confidence: 99%

Recent trends in detecting, controlling, and detoxifying of patulin mycotoxin using biotechnology methods

Ngea

Yang

Castoria

et al. 2020

Comp Rev Food Sci Food Safe

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Patulin (PAT) is a mycotoxin that can contaminate many foods and especially fruits and fruit‐based products. Therefore, accurate and effective testing is necessary to enable producers to comply with regulations and promote food safety. Traditional approaches involving the use of chemical compounds or physical treatments in food have provided practical methods that have been used to date. However, growing concerns about environmental and health problems associated with these approaches call for new alternatives. In contrast, recent advances in biotechnology have revolutionized the understanding of living organisms and brought more effective biological tools. This review, therefore, focuses on the study of biotechnology approaches for the detection, control, and mitigation of PAT in food. Future aspects of biotechnology development to overcome the food safety problem posed by PAT were also examined. We find that biotechnology advances offer novel, more effective, and environmental friendly approaches for the control and elimination of PAT in food compared to traditional methods. Biosensors represent the future of PAT detection and use biological tools such as aptamer, enzyme, and antibody. PAT prevention strategies include microbial biocontrol, the use of antifungal biomolecules, and the use of microorganisms in combination with antifungal molecules. PAT detoxification aims at the breakdown and removal of PAT in food by using enzymes, microorganisms, and various adsorbent biopolymers. Finally, biotechnology advances will be dependent on the understanding of fundamental biology of living organisms regarding PAT synthesis and resistance mechanisms.

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Screening and development of DNA aptamers as capture probes for colorimetric detection of patulin

Cited by 95 publications

References 28 publications

Development of an Impedimetric Aptasensor for Label Free Detection of Patulin in Apple Juice

Development of an Impedimetric Aptasensor for Label Free Detection of Patulin in Apple Juice

Aptamers used for biosensors and targeted therapy

Recent trends in detecting, controlling, and detoxifying of patulin mycotoxin using biotechnology methods

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