Screening and differential oxidative stress responses of hot pepper (Capsicum annuum L.) genotypes under cold stress

Nandagopal, Jaya Ganesh Thiruvengadam; Suja, G.

doi:10.1016/j.sajb.2022.07.013

Cited by 4 publications

(3 citation statements)

References 49 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In pepper production, various antistressors are commonly employed to mitigate abiotic stresses [38][39][40][41]. Additionally, chilling acclimation is utilized to enhance pepper tolerance to low temperatures [42]. This phenomenon, also known as "stress memory" [43], represents the plasticity mechanism of plants in response to production conditions.…”

Section: Discussionmentioning

confidence: 99%

Comprehensive analysis of pepper (Capsicum annuum) RAV genes family and functional identification of CaRAV1 under chilling stress

Pei,

Yang,

et al. 2024

Preprint

View full text Add to dashboard Cite

Background The RAV gene family in Capsicum annuum remains largely unexplored in response to chilling stress, despite its known significance in plant abiotic stress responses. Results In this study, we identified and characterized six members of the CaRAVs gene subfamily in pepper through genome-wide analysis. Subsequently, the CaRAVs subfamily was classified into four branches based on homology with Arabidopsis thaliana, each exhibiting relatively conserved domains within the branch. We discovered that light response elements accounted for the majority of CaRAVs, whereas low-temperature response elements were specific to the NGA gene subfamily. After pepper plants were subjected to chilling stress, qRT-PCR analysis revealed that CaRAV1, CaRAV2 and CaNGA1 were significantly induced in response to chilling stress, indicating that CaRAVs play a role in the response to chilling stress. Using virus-induced gene silencing (VIGS) vectors, we targeted key members of the CaRAVs gene family, resulting in plants with increased susceptibility to chilling damage and reduced antioxidant enzyme activity, particularly evident under repeated chilling stress. These findings suggest that CaRAV1 and CaNGA1 positively regulate the response to chilling stress. Conclusions Silencing targeting key members of the CaRAVs gene family, results in plants with increased susceptibility to chilling damage and reduced antioxidant enzyme activity in plants, particularly evident under repeated chilling stress. This present study provides valuable information for understanding the classification and putative functions of the RAV transcription factors in ppepper.

show abstract

Section: Discussionmentioning

confidence: 99%

Comprehensive analysis of pepper (Capsicum annuum) RAV genes family and functional identification of CaRAV1 under chilling stress

Pei,

Yang,

et al. 2024

Preprint

View full text Add to dashboard Cite

show abstract

Section: Enhancement Of Capsicum Secondary Metabolites Via Abiotic An...mentioning

confidence: 99%

“…Cold stress (10/8 °C, day/night) led to an increment in the antioxidant enzymes in all 12 C. annuum genotypes essentially to maintain the capsaicinoids biosynthesis pathway, except in CA-173 and F-02 where capsaicinoid levels were decimated. 128 Temperature-controlled plastic tunnels improved carotenoid and ascorbic acid levels in red sweet peppers, whereas white shade nets favored phenolic compounds. 129 Cold stress induced carotenoid biosynthesis via up regulating the gene expression of carotenoid biosynthesis genes such as LCYB, PSY, ZEP, and β-carotene hydroxylase in CaATHB-12 silenced C. annuum fruit.…”

Section: Elicitor Treatment and Mineralmentioning

confidence: 99%

Genetic Regulation, Environmental Cues, and Extraction Methods for Higher Yield of Secondary Metabolites in Capsicum

Islam

Rawoof

Kumar

et al. 2023

J. Agric. Food Chem.

View full text Add to dashboard Cite

Capsicum (chili pepper) is a widely popular and highly consumed fruit crop with beneficial secondary metabolites such as capsaicinoids, carotenoids, flavonoids, and polyphenols, among others. Interestingly, the secondary metabolite profile is a dynamic function of biosynthetic enzymes, regulatory transcription factors, developmental stage, abiotic and biotic environment, and extraction methods. We propose active manipulable genetic, environmental, and extraction controls for the modulation of quality and quantity of desired secondary metabolites in Capsicum species. Specific biosynthetic genes such as Pun (AT3) and AMT in the capsaicinoids pathway and PSY, LCY, and CCS in the carotenoid pathway can be genetically engineered for enhanced production of capsaicinoids and carotenoids, respectively. Generally, secondary metabolites increase with the ripening of the fruit; however, transcriptional regulators such as MYB, bHLH, and ERF control the extent of accumulation in specific tissues. The precise tuning of biotic and abiotic factors such as light, temperature, and chemical elicitors can maximize the accumulation and retention of secondary metabolites in pre-and postharvest settings. Finally, optimized extraction methods such as ultrasonication and supercritical fluid method can lead to a higher yield of secondary metabolites. Together, the integrated understanding of the genetic regulation of biosynthesis, elicitation treatments, and optimization of extraction methods can maximize the industrial production of secondary metabolites in Capsicum.

show abstract

BoPRR9, a pseudo-response regulator protein from cabbage, plays a negative regulatory role in the response to cold stress

Xing,

Raza,

et al. 2024

Environmental and Experimental Botany

View full text Add to dashboard Cite

Screening and differential oxidative stress responses of hot pepper (Capsicum annuum L.) genotypes under cold stress

Cited by 4 publications

References 49 publications

Comprehensive analysis of pepper (Capsicum annuum) RAV genes family and functional identification of CaRAV1 under chilling stress

Comprehensive analysis of pepper (Capsicum annuum) RAV genes family and functional identification of CaRAV1 under chilling stress

Genetic Regulation, Environmental Cues, and Extraction Methods for Higher Yield of Secondary Metabolites in Capsicum

BoPRR9, a pseudo-response regulator protein from cabbage, plays a negative regulatory role in the response to cold stress

Contact Info

Product

Resources

About