Screening and identification of differentially expressed transcripts in circulating cells of prostate cancer patients using suppression subtractive hybridization

Li, Xin; Wong, Carson; Mysel, Ralph; Slobodov, Gennady; Metwalli, Adam R.; Kruska, Jarrett; Manatt, C. Scott; Culkin, Daniel J.; Kropp, Bradley P.; Lin, Hsueh Kung

doi:10.1186/1476-4598-4-30

Cited by 5 publications

(3 citation statements)

References 40 publications

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“…These authors (38) suggest that LNCaP cells should be a good model for many aspects of the study of prostate cancers. Several microarray studies have attempted to determine patterns of gene expression that predict therapeutic response (38)(39)(40)(41). In general, this work has identified patterns of differentially expressed genes, but so far it has not been able to establish a set of marker genes that can reliably predict therapeutic response.…”

Section: Discussionmentioning

confidence: 99%

“…In general, this work has identified patterns of differentially expressed genes, but so far it has not been able to establish a set of marker genes that can reliably predict therapeutic response. It is encouraging that gene expression profiling appears to be possible with circulating tumor cells, which clearly are more accessible than pre-and posttherapy prostate biopsy material (40,41).…”

Section: Discussionmentioning

confidence: 99%

“…The studies reported to date (40,41) indicate that an assay for specific proteins in circulating tumor cells could be a promising technique and clearly one that requires further development. This work suggests that assays of specific proteins, such as Bcl-2, TBP-2, and cytochrome c, could be useful guides to therapeutic markers and for which immunohistochemical assay techniques are available.…”

Section: Discussionmentioning

confidence: 99%

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Intrinsic apoptotic and thioredoxin pathways in human prostate cancer cell response to histone deacetylase inhibitor

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et al. 2006

Proc. Natl. Acad. Sci. U.S.A.

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There is a great need to develop better mechanism-based therapies for prostate cancer. In this investigation, we studied four human prostate cancer cell lines, LNCaP, DU145, LAPC4, and PC3, which differ in response to the histone deacetylase inhibitor, suberoylanilide hydroxamic acid (vorinostat), a new anticancer drug. Examining the role of intrinsic mitochondrial caspase-dependent apoptosis and caspase-independent, reactive oxygen species (ROS) facilitated cell death, has provided an understanding of mechanisms that may determine the varied response to the histone deacetylase inhibitor. We found striking differences among these cancer cells in constitutive expression and response to suberoylanilide hydroxamic acid in levels of antiapoptotic and proapoptotic proteins, mitochondria membrane integrity, activation of caspases, ROS accumulation, and expression of thioredoxin, the major scavenger of ROS. Identifying these differences can have predictive value in assessing therapeutic response and identifying targets to enhance therapeutic efficacy. mitochondria membrane ͉ reactive oxygen species ͉ suberoylanilide hydroxamic acid ͉ thioredoxin-binding protein ͉ caspase

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Intrinsic apoptotic and thioredoxin pathways in human prostate cancer cell response to histone deacetylase inhibitor

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Pérez

et al. 2006

Proc. Natl. Acad. Sci. U.S.A.

170

160

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Proteome analysis of human androgen‐independent prostate cancer cell lines: Variable metastatic potentials correlated with vimentin expression

Bai

et al. 2007

Proteomics

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To better understand the molecular mechanisms of prostate cancer (PCA) dissemination and to develop new anti-metastasis therapies, key regulatory molecules involved in PCA metastasis were identified in two human androgen-independent PCA cell lines, highly metastatic 1E8-H and lowly metastatic 2B4-L cells. Through 2-DE and MS analyses, 12 proteins with different expression levels in the two cell lines were identified. The following proteins were found to be significantly up-regulated in 1E8-H cells compared with 2B4-L cells: gp96 precursor, calreticulin precursor, vimentin (VIM), Hsp90alpha, peroxiredoxin 2, HNRPH1, ezrin, T-complex protein 1, alpha subunit, and hypothetical protein mln2339. In contrast, heart L-lactate dehydrogenase H chain, annexin I, and protein disulfide isomerase were notably down-regulated in 1E8-H cells compared with 2B4-L cells. To our knowledge, this study is the first to demonstrate that up-regulation of VIM expression positively correlates with the invasion and metastasis of androgen-independent PCA.

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Discovery and development of SAHA as an anticancer agent

2007

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The path to the discovery of suberoylanilide hydroxamic acid (SAHA, vorinostat) began over three decades ago with our studies designed to understand why dimethylsulfoxide causes terminal differentiation of the virus-transformed cells, murine erythroleukemia cells. SAHA can cause growth arrest and death of a broad variety of transformed cells both in vitro and in vivo at concentrations that have little or no toxic effects on normal cells. It was discovered that SAHA inhibits the activity of histone deacetylases (HDACs), including all 11 known human class I and class II HDACs. HDACs have many protein targets whose structure and function are altered by acetylation including histones and non-histone proteins component of transcription factors controlling gene expression and proteins that regulate cell proliferation, migration and death. SAHA is in clinical trials and has significant anticancer activity against both hematologic and solid tumors at doses well tolerated by patients. A new drug application has been approved for SAHA (vorinostat) treatment of cutaneous T-cell lymphoma.

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Screening and identification of differentially expressed transcripts in circulating cells of prostate cancer patients using suppression subtractive hybridization

Cited by 5 publications

References 40 publications

Intrinsic apoptotic and thioredoxin pathways in human prostate cancer cell response to histone deacetylase inhibitor

Intrinsic apoptotic and thioredoxin pathways in human prostate cancer cell response to histone deacetylase inhibitor

Proteome analysis of human androgen‐independent prostate cancer cell lines: Variable metastatic potentials correlated with vimentin expression

Discovery and development of SAHA as an anticancer agent

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