Screening and Isolation of Cellulolytic Bacteria from Gut of Black Soldier Flays Larvae (Hermetia illucens) Feeding with Rice Straw

Supriyatna, Ateng; Ukit, Ukit

doi:10.15294/biosaintifika.v8i3.6762

Cited by 20 publications

(19 citation statements)

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“…Cellulose, another main component of the diet supplied, could play a role in BSF nutrition. Lee et al (2014) and Supriyatna and Ukit (2016) studied the digestive capabilities of BSF and reported cellulase activity in the gut microflora of the species, produced by cellulolytic bacteria, in particular Bacillus spp., hence BSF could be using cellulose nutritionally. However, the quantity of cellulose that BSF ingests and digests has not been quantified.…”

Section: Larval Performancementioning

confidence: 99%

Flies are what they eat : Tailoring nutrition of Black Soldier Fly (Hermetia illucens L.) for larval biomass production and fitness

Barragán-Fonseca

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Black Soldier Fly-BSF; Hermetia illucens L. (Diptera: Stratiomyidae) has been proposed as one of the most suitable insect species to be used as animal feed. Advantages of using BSF that have been identified are their capacity to convert organic waste, to reduce the numbers of certain harmful bacteria and insect pests, to provide potential chemical precursors to produce biodiesel, and to provide high quality protein to be used as feed for a variety of animals. This thesis explores nutritional physiology of BSF and its effect on larval biomass production and fitness. Knowing the physiological mechanisms that BSF employs to deal with different nutrient concentrations, and their impacts on BSF life-history traits and larval body content, may yield valuable insights into the nutritional ecology of BSF. Experiments involving the study of larval densities, total nutrient content, and dietary protein (P) and carbohydrate (C) content and ratio on artificial, semi-artificial and organic residual stream diets were used to unravel their effect on life-history traits, body nutrient content and reproduction of BSF. Results presented in this thesis show that larval density and nutrient content interact at low nutrient content to affect BSF larval performance and body composition, nutrient content overall having a stronger effect. Regarding dietary protein and carbohydrate, I conclude that BSF has post-ingestive mechanisms to deal with imbalanced foods. Carbohydrate-biased P:C ratios positively affect BSF performance, both on the artificial diets and the organic residue stream, P-content being limiting for most of the performance variables. In the experiments throughout this study larval protein content was high and largely independent of dietary protein content, although small differences were found between treatments. However, larval crude fat varied over a wider range depending on dietary macronutrient content, protein content affecting larval crude fat content to a large extent. The data presented in this thesis contribute to our understanding of how nutrition, mainly in terms of protein and carbohydrate content and ratio, affects life-history traits, nutrient body content and reproduction of BSF and how this knowledge can be applied to improve the productive performance and larval body composition in BSF production systems.

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Section: Larval Performancementioning

confidence: 99%

Flies are what they eat : Tailoring nutrition of Black Soldier Fly (Hermetia illucens L.) for larval biomass production and fitness

Barragán-Fonseca

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“…Keberadaan bakteri dalam usus larva tersebut membantu BSF dalam mengkonversi limbah organik menjadi lemak dan protein menjadi biomassa tubuhnya (Zheng et al, 2011). Supriyatna & Ukit (2016) menunjukan bahwa pada usus larva BSF terdapat bakteri B. subitilis yang dapat memproduksi amilase. Sementara, Kim et al(2011) menemukan adanya potensi amilase, lipase, dan protease dari ekstrak usus larva BSF.…”

Section: Pendahuluanunclassified

“…Uji aktivitas amilase dilakukan dengan metode DNS. Ke dalam tabung yang telah diisi 1 mL substrat pati 1% dimasukkan 1 mL ekstrak kasar enzim, kemudian dihomogenkan menggunakan vortex dan diinkubasi selama 30 menit dalam shaker incubator pada suhu 40 ºC (Supriyatna & Ukit, 2016). Reaksi dihentikan dengan menambahkan 1 mL reagen DNS dan dihomogenkan dengan vortex.…”

Section: Uji Aktivitas Amilaseunclassified

Aktivitas amilase bakteri amilolitik asal larva black soldier fly (Hermetia illucens)

Kresnawaty¹,

Wahyu

Sasongko

2019

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Amylase is an enzyme that has been widely used as a biocatalyst in foodand bioethanol industries. The availability of thermostable amylase will further expand the market and extend the shelf life of this enzyme. Amylase is produced by amylolytic bacteria using media with high-costnitrogen sources, such as pepton. Black soldier fly (BSF) is a potential source of amylolitic bacteria since its ability to degrade organic matters rapidly. This research aimedtoexploreamylolitic bacteria from the larvae of BSF with highest amylase activity that can be produced using low-cost media. The screening ofamylase activity was conducted by culturing the bacteria on starch containing media.Bacteria with the highest amylase activity were cultured in liquid media with twodifferentnitrogensources (urea and nitrate). Determinations of the optimum pH and temperature for this enzyme activity were carried out in the pH range 4to 7 and temperature 35to 65 ºC. Three amylase-producing isolates were obtained in this study. M1 isolate which has the highest activity was characterized based oncatalase activity and Gram staining. The results showed that the M1 isolate mightbelong togenus Proteussp. At the optimum condition (45ºC and pH 7), amylase activityin nitrate mediawas0.791U/mL, which was about 18-folds higher than that in ureamedia (0,041U/mL). Thus, amylase isolated from BSF larvae can be classified as a mesophilic enzyme and has the potential to be developed commercially at lower production costs.[Keywords:crude extract enzyme,Proteus sp.,thermostable] AbstrakAmilase merupakan salah satu enzim yangtelah digunakan secara luas sebagai biokatalis dalam industri pangan dan bioetanol.Ketersediaan amilase termostabil akan semakin memperluas pasar dan memperpanjang daya simpan enzim ini. Selama ini, produksi amilase dilakukan dengan memanfaatkan bakteri amilolitik menggunakan media dengan sumber nitrogen yang mahal, misalnya pepton. Black soldier fly (BSF) merupakan sumber bakteri amilolitik yang potential karena BSF memiliki kemampuan mendegradasi bahan organik dengan cepat. Penelitian ini bertujuan untuk mengeksplorasi bakteri amilolitik dengan kemampuan amilase tinggi yang dapat diproduksi menggunakan media yanglebihmurah.Skrining bakteri penghasil amilase dilakukan dengan menumbuhkan bakteri pada media yang mengandung pati. Bakteri dengan aktivitas amilase tertinggi dikulturkan dalam media cair dengan dua sumber nitrogenyang berbeda, yaitu urea dan nitrat. PenentuanpH dan suhu optimum aktivitas enzim ini dilakukan pada rentang pH 4sampai 7 dan suhu 35sampai 65 ºC.Tiga isolat penghasil amilase diperoleh dalam penelitian ini. Isolat M1 yang memiliki aktivitas tertinggi dikarakterisasi berdasarkan uji katalasedan uji pewarnaan Gram. Hasilnya menunjukkan bahwa isolat M1 termasukgenus Proteus sp. Pada kondisi optimum (suhu 45oC dan pH 7), aktivitas amilase pada media nitrat adalah 0,791 U/mL, lebih kurang 18 kali lebih tinggi dibanding aktivitas pada media urea (0,041 U/mL). Dengan demikian, amilase yang dihasilkan oleh bakteri asallarva BSF merupakan enzim mesofilik dan berpotensi untuk dikembangkan secara komersial dengan biaya produksi yang lebih murah.[Kata kunci: enzim ekstrak kasar, Proteussp.,termostabil]

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“…The contained microbes in the intestines of fish have amylolytic activity, (digesting carbohydrates) proteolytic, (digesting protein), and lipolytic (digesting fat). Based on the study that was conducted by Supriyatna & Ukit (2016), the type of cellulolytic bacteria was found in the intestines of Hermetia illucens larvae.…”

Section: Introductionmentioning

confidence: 99%

The Isolation and Identification Bacteria on Jallalah Animal (Study on the Feeding Tilapia (Oreochromis niloticus) with Chicken Manure As Foods)

Kurahman

Yuliawati

Haerunnisa

et al. 2020

EKW

Self Cite

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In the hadith, Rasulullah SAW prohibit human to consume meat and milk from manure-eating animal (Jallalah animal). In this study, tilapia fishes were fed chicken manure (being Jallalah). Then, it was analyzed based on the microbe in their intestine. The purposes of this study are to isolate and to identify the types of bacteria, and to determine the pathogenic and non-pathogenic bacteria in the intestines of fish that have been fed chicken manure. Bacteria samples were isolated from the intestine of fish with chicken manure and the commercial fish food as a control. Bacteria identification was done by using morphological characterization, macroscopic and microscopic identification, and biochemical test. The results show that 21 isolated bacteria in the fish intestine that was fed chicken manure and six of the bacteria are pathogenic. Based on this study, it can be concluded that tilapia fish by feeding with chicken manure contains many types of pathogens bacteria. Briefly, tilapia fish is not good for being consumption.Abstrak: Dalam hadis yang diriwayatkan oleh Ibnu Umar, Rasulullah SAW. Melarang memakan daging dan meminum susu hewan pemakan kotoran (hewan Jallalah). Pada penelitian ini, ikan nila diberi pakan kotoran ayam (menjadi Jallalah). Selanjutnya ikan tersebut dianalisis kandungan mikroba yang terkandung didalam saluran pencernaannya. Tujuan penelitian ini adalah mengisolasi dan mengidentifikasi bentuk bakteri dan mendeterminasi bakteri patogen dan non-patogen yang terdapat didalam saluran pencernaan ikan ynag telah diberi pakan kotoran ayam. Sampel bakteri diisolasi dari saluran pencernaan ikan setelah diberi kotoran ayam dan ikan yang diberi pakan komersil (kontrol). Identifikasi bakteri dilakukan secara makroskopik, mikroskopik, dan uji biokimia. Hasil penelitian menunjukkan ditemukan 21 isolat bakteri dalam usus ikan yang diberi korotan ayam yang tergolong ke dalam genus Listeria, Staphylococcus, Alcaligenes, Cromobacterium, Edwardisiella, Micrococcus, Bacillus, dan Kurthia. Dari 21 jenis tersebut terdapat 6 jenis bakteri pathogen yaitu Edwarsiella sp, Micrococcus sp, Staphylococcus sp, Alcaligenes sp, Listeria sp dan Chromobacterium. Kesimpulan pada penelitian ini bahwa hewan ikan yang memakan kotoran ayam mengandung banyak jenis bakteri patogen sehingga tidak baik untuk di konsumsi.

show abstract

Screening and Isolation of Cellulolytic Bacteria from Gut of Black Soldier Flays Larvae (Hermetia illucens) Feeding with Rice Straw

Cited by 20 publications

References 0 publications

Flies are what they eat : Tailoring nutrition of Black Soldier Fly (Hermetia illucens L.) for larval biomass production and fitness

Flies are what they eat : Tailoring nutrition of Black Soldier Fly (Hermetia illucens L.) for larval biomass production and fitness

Aktivitas amilase bakteri amilolitik asal larva black soldier fly (Hermetia illucens)

The Isolation and Identification Bacteria on Jallalah Animal (Study on the Feeding Tilapia (Oreochromis niloticus) with Chicken Manure As Foods)

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