Screening and Isolation of Protease Producing Bacteria from Rhizospheric Soil of Apple Orchards from Shimla District (HP), India

Manorma, Kumari; Sharma, Shweta; Singla, Himani; Kaundal, Kirti; Kaur, Mohinder

doi:10.20546/ijcmas.2017.605.030

Cited by 6 publications

(5 citation statements)

References 6 publications

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“…Morpho-biochemical tests showed that the best isolate was identified as Pseudomonas sp, however 16SrRNA sequence analysis declared the isolate as Pseudomonas fluorescens. Further molecular characterization of isolates is preferred over morpho-biochemical tests for the reason of being the best identification method [34,35]. Previous investigations related to morpho-biochemical characterization of the bacterial strains reported Bacillus sp and Pseudomonas sp [36,37].…”

Section: Discussionmentioning

confidence: 99%

Isolation and characterization of psychrotrophic proteolytic bacteria from landfill site under temperate climatic conditions of Kashmir Himalaya

Hamid

Jehangir

Baba

et al. 2019

Preprint

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The temperate climatic regions face the problem of waste accumulation due to lower environmental temperatures. However, these regions harbor cold active microbes viz. psychrotrophic proteolytic bacteria that play an important role in the degradation of protenaceous materials of the waste stream. Hence in the present study psychrotrophic proteolytic bacteria were isolated from waste samples collected from landfill site by using random sampling method under environmental temperature of 10oC. By using serial dilution and spread plate technique a total of 8 morphologically different psychrotrophic proteolytic bacteria were isolated on skim milk agar media at pH of 7.0 and temperature of 10°C after 48hours. Under in-vitro conditions all the isolates produced significant quantities of protease over the control and diameters of hydrolysis zones ranged between 2 to 18 mm at temperature range of 5 to 20oC and after 72 hours. The corresponding quantitative protease activities of the isolates was significant that ranged between 0.5 to 2.25 U/ml and the isolate PB2 was most efficient with highest protease activity of 2.25U/ml at 20oC. Based on 16SrRNA analysis the isolate was identified as Pseudomonas florescence with 96% similarity. It was concluded that the isolates can grow in wide ranges of temperature and could be used for enhanced decomposition of organic wastes during lower temperature conditions in cold regions. Further the isolates could have industrial applications due to the production of cold active proteases that would help economic benefits through energy conservation.

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Section: Discussionmentioning

confidence: 99%

Isolation and characterization of psychrotrophic proteolytic bacteria from landfill site under temperate climatic conditions of Kashmir Himalaya

Hamid

Jehangir

Baba

et al. 2019

Preprint

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“…The insoluble portion was dissolved by the addition of the alkali. The pH was adjusted to 8.0 with 0.1 M NaOH [10] . The casein was then added into 2% of bacto agar to have the final concentration of 3%, followed by sterilization at 121 °C for 20 min.…”

Section: Experimental Design Materials and Methodsmentioning

confidence: 99%

Technical data on the inhibition properties of some medicinal plant extracts towards caseinolytic protease proteolytic subunit of Plasmodium knowlesi

et al. 2021

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“…amylase test, catalase test, carbohydrate utilization test, MR and VP test, urease production test, citrate test, nitrate reduction test, H2S production test (Happy et al, 2018). Moreover, different physiological factors such as pH, temperature, nitrogen and the caron sources were also included to characterized the isolated protease bacteria (Dalal, 2015).…”

Section: Identification Of Bacteriamentioning

confidence: 99%

Screening and Characterization of Protease Producing Bacteria from Raw Milk Sample

2023

IJFMR

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The purpose of the current research was to screen, characterize and isolate protease producing bacteria from raw milk sample. Initially, the raw milk sample was spread on nutrient agar, at 35oC for 20 hrs. Four bacterial colonies of different morphology for further study. Among these, M-4 colony were selected and isolate produced highest protease activity and was identified as Proteus mirabilis by morphological and biochemical tests. Moreover, various physiological characters were also studied such as pH, temperature, carbon source and nitrogen sources. The bacteria showed maximum activity at pH 7, temperature requirement is 35oC, lactose as a carbon source and potassium nitrate as a nitrogen source respectively. At the end, enzyme activity assay was done by using well diffusion method. Microbial proteases which are environmentally friendly are interestingly used for their commercial importance.

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Screening and Isolation of Protease Producing Bacteria from Rhizospheric Soil of Apple Orchards from Shimla District (HP), India

Cited by 6 publications

References 6 publications

Isolation and characterization of psychrotrophic proteolytic bacteria from landfill site under temperate climatic conditions of Kashmir Himalaya

Isolation and characterization of psychrotrophic proteolytic bacteria from landfill site under temperate climatic conditions of Kashmir Himalaya

Technical data on the inhibition properties of some medicinal plant extracts towards caseinolytic protease proteolytic subunit of Plasmodium knowlesi

Screening and Characterization of Protease Producing Bacteria from Raw Milk Sample

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