Screening and optimization of nutritional factors for higher dextransucrase production by Leuconostocmesenteroides NRRL B-640 using statistical approach

Purama, Ravi Kiran; Goyal, Arun

doi:10.1016/j.biortech.2008.01.032

Cited by 42 publications

(37 citation statements)

References 26 publications

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“…There is no literature available about the optimization of medium composition for dextransucrase production from Weissella confusa. Leuconostoc mesenteroides NRRL B-640 produced 10.7 U/ml dextransucrase activity in the optimised medium [17]. However according to a recent study by Patel et al, 2011 [18] a mutant Pediococcus pentosaceus (SPAm) showed substantially higher dextransucrase activity (15.6 U/ml).…”

Section: Optimum Concentration Levelmentioning

confidence: 99%

Development of Efficient Fermentation Process at Bioreactor Level by Taguchi's Orthogonal Array Methodology for Enhanced Dextransucrase Production from <i>Weissella confusa</i> Cab3

Shukla¹,

Goyal²

2012

AiM

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The influence of medium ingredients on extracellular dextransucrase production by a new bacterial strain Weissella confusa Cab3 (Genbank Accession Number JX649223) was evaluated using fractional factorial design of Taguchi's orthogonal array. Four metabolism influencing factors viz. sucrose, yeast extract, K 2 HPO 4 and Tween80 were selected to optimize dextransucrase production by W. confusa Cab3 using fractional factorial design of Taguchi methodology. Based on the influence of interaction components of fermentation, least significant factors of individual level have higher interaction severity index and vice versa for enzyme production from Weissella confusa Cab3. Sucrose and yeast extract were found to be the most significant factors which positively influenced the dextransucrase production. The optimized medium composition consisted of sucrose-5%; yeast extract-2%; K 2 HPO 4 -1.0%; Tween80-0.5, based on Taguchi orthogonal array method. The optimized composition gave an experimental value of dextransucrase activity of 17.9 U/ml at shake flask level which corresponded well with the predicted value of 17.54 U/ml by the model. The optimized medium by Taguchi method gave significant (3 fold) enhancement of dextransucrase activity as compared to unoptimised enzyme activity of 6.0 U/ml. The dextransucrase production was scaled up in lab scale bioreactor resulting in further enhancement of enzyme activity (22.0 U/ml).

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Section: Optimum Concentration Levelmentioning

confidence: 99%

Development of Efficient Fermentation Process at Bioreactor Level by Taguchi's Orthogonal Array Methodology for Enhanced Dextransucrase Production from <i>Weissella confusa</i> Cab3

Shukla¹,

Goyal²

2012

AiM

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“…These wide variations in recombinant Xyn2 activity were coupled with narrow variations in biomass that ranged from 4.08±0.18 to 5.85±0.03 g/l ( Table 2). The wide variation in recombinant Xyn2 production reflected the significance of factors on the enzyme and biomass production (Purama and Goyal 2008) and vice versa. Comparisons among these eleven trials showed that the medium in trial 4 produced the highest recombinant Xyn2 activity, while the medium in trial 10 produced the highest biomass.…”

Section: Preliminary Experimentsmentioning

confidence: 99%

Development and validation of a medium for recombinant endo-β-1,4-xylanase production by Kluyveromyces lactis using a statistical experimental design

et al. 2011

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Kluyveromyces lactis is an excellent host for a high cell density culture, which allows high expression levels of recombinant enzymes. Nutrient composition and culture conditions affect the secretion, production level and stability of the recombinant host. Therefore, it is technologically important to formulate a medium that stimulates high cell density and enhances the desired enzyme production using K. lactis GG799. In this study, six media were initially compared, and a Plackett-Burman experimental design was employed to screen for important components and trace elements. Nitrogen sources such as ammonium sulfate and free amino acid (casamino acid) as well as compounds like MgSO 4 •7H 2 O, Na 2 SO 4 , ZnSO 4 •6H 2 O, MnSO 4 •4H 2 O and KH 2 PO 4 affected biomass concentrations (5.67 g/l) and recombinant endo-β-1,4

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“…Amongst various nutritional requirements, carbon source and nitrogen source are generally regarded as important factors of metabolism, and several examples of the production of metabolites in media with optimized contents of these components are also described in the literature (Purama and Goyal, 2008;Yuan et al, 2008).…”

Section: Validation Of the Optimized Conditionmentioning

confidence: 99%

Medium optimization for the production of avermectin B1a by Streptomyces avermitilis 14-12A using response surface methodology

Gao

Liu

et al. 2009

Bioresource Technology

137

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Screening and optimization of nutritional factors for higher dextransucrase production by Leuconostocmesenteroides NRRL B-640 using statistical approach

Cited by 42 publications

References 26 publications

Development of Efficient Fermentation Process at Bioreactor Level by Taguchi's Orthogonal Array Methodology for Enhanced Dextransucrase Production from <i>Weissella confusa</i> Cab3

Development of Efficient Fermentation Process at Bioreactor Level by Taguchi's Orthogonal Array Methodology for Enhanced Dextransucrase Production from <i>Weissella confusa</i> Cab3

Development and validation of a medium for recombinant endo-β-1,4-xylanase production by Kluyveromyces lactis using a statistical experimental design

Medium optimization for the production of avermectin B1a by Streptomyces avermitilis 14-12A using response surface methodology

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Screening and optimization of nutritional factors for higher dextransucrase production by Leuconostocmesenteroides NRRL B-640 using statistical approach

Cited by 42 publications

References 26 publications

Development of Efficient Fermentation Process at Bioreactor Level by Taguchi's Orthogonal Array Methodology for Enhanced Dextransucrase Production from &lt;i&gt;Weissella confusa&lt;/i&gt; Cab3

Development of Efficient Fermentation Process at Bioreactor Level by Taguchi's Orthogonal Array Methodology for Enhanced Dextransucrase Production from &lt;i&gt;Weissella confusa&lt;/i&gt; Cab3

Development and validation of a medium for recombinant endo-β-1,4-xylanase production by Kluyveromyces lactis using a statistical experimental design

Medium optimization for the production of avermectin B1a by Streptomyces avermitilis 14-12A using response surface methodology

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Development of Efficient Fermentation Process at Bioreactor Level by Taguchi's Orthogonal Array Methodology for Enhanced Dextransucrase Production from <i>Weissella confusa</i> Cab3

Development of Efficient Fermentation Process at Bioreactor Level by Taguchi's Orthogonal Array Methodology for Enhanced Dextransucrase Production from <i>Weissella confusa</i> Cab3