Screening and optimization of polyhydroxybutyrate (PHB) by Lysinibacillus fusiformis from diverse environmental sources

Adebajo, Seun Owolabi; Bankole, Paul Olusegun; Ojo, Abidemi Esther; Akintokun, Pius Olugbenga

doi:10.1016/j.microb.2024.100043

Cited by 3 publications

(1 citation statement)

References 22 publications

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“…However, the size may vary depending on the species, culture medium, and growth conditions. On the other hand, agricultural soil has been differentiated from other soils by reporting higher numbers of PHB-producing isolates [14].…”

Section: Introductionmentioning

confidence: 99%

Alkaline-Tolerant Bacillus cereus 12GS: A Promising Polyhydroxybutyrate (PHB) Producer Isolated from the North of Mexico

San Miguel-González,

Alemán-Huerta,

Martínez-Herrera

et al. 2024

Microorganisms

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Environmental pollution caused by petroleum-derived plastics continues to increase annually. Consequently, current research is interested in the search for eco-friendly bacterial polymers. The importance of Bacillus bacteria as producers of polyhydroxyalkanoates (PHAs) has been recognized because of their physiological and genetic qualities. In this study, twenty strains of Bacillus genus PHA producers were isolated. Production was initially evaluated qualitatively to screen the strains, and subsequently, the strain B12 or Bacillus sp. 12GS, with the highest production, was selected through liquid fermentation. Biochemical and molecular identification revealed it as a novel isolate of Bacillus cereus. Production optimization was carried out using the Taguchi methodology, determining the optimal parameters as 30 °C, pH 8, 150 rpm, and 4% inoculum, resulting in 87% and 1.91 g/L of polyhydroxybutyrate (PHB). Kinetic studies demonstrated a higher production within 48 h. The produced biopolymer was analyzed using Fourier-transform infrared spectroscopy (FTIR), confirming the production of short-chain-length (scl) polyhydroxyalkanoate, named PHB, and differential scanning calorimetry (DSC) analysis revealed thermal properties, making it a promising material for various applications. The novel B. cereus isolate exhibited a high %PHB, emphasizing the importance of bioprospecting, study, and characterization for strains with biotechnological potential.

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Section: Introductionmentioning

confidence: 99%

Alkaline-Tolerant Bacillus cereus 12GS: A Promising Polyhydroxybutyrate (PHB) Producer Isolated from the North of Mexico

San Miguel-González,

Alemán-Huerta,

Martínez-Herrera

et al. 2024

Microorganisms

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Statistical Design-Driven Optimization of Polyhydroxy-Butyrate (PHB) Production Using Sugarcane Bagasse by Brevibacterium sp. (PP989436) Strain

Preethi Rathna,

Naveen Kishore,

Kulandhaivel

2024

J Polym Environ

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Producing and Characterizing Polyhydroxyalkanoates from Starch and Chickpea Waste Using Mixed Microbial Cultures in Solid-State Fermentation

Grgurević,

Bramberger,

Miloloža

et al. 2024

Polymers

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The environmental impact of plastic waste is a growing global challenge, primarily due to non-biodegradable plastics from fossil resources that accumulate in ecosystems. Biodegradable polymers like polyhydroxyalkanoates (PHAs) offer a sustainable alternative. PHAs are microbial biopolymers produced by microorganisms using renewable substrates, including agro-industrial byproducts, making them eco-friendly and cost-effective. This study focused on the isolation and characterization of PHA-producing microorganisms from agro-industrial waste, including chickpeas, chickpeas with bean residues, and starch. Screening via Sudan Black staining identified PHA-accumulating strains such as Brevibacillus sp., Micrococcus spp., and Candida krusei, among others. To assess the potential for PHA biosynthesis, solid-state fermentation (SSF) was conducted using agro-industrial waste as substrates, along with a mixed culture of the isolated microorganisms. The highest observed yield was a PHA accumulation of 13.81%, achieved with chickpeas containing bean residues. Structural and thermal characterization of the PHAs was performed using Fourier-transform infrared spectroscopy with attenuated total reflectance (FTIR-ATR), differential scanning calorimetry (DSC), and thermogravimetric analysis (TGA). FTIR-ATR spectra indicated polyhydroxybutyrate (PHB), suggesting it as the synthesized PHA type. This study highlights the potential of agro-industrial waste for sustainable PHA production and eco-friendly bioplastics.

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Screening and optimization of polyhydroxybutyrate (PHB) by Lysinibacillus fusiformis from diverse environmental sources

Cited by 3 publications

References 22 publications

Alkaline-Tolerant Bacillus cereus 12GS: A Promising Polyhydroxybutyrate (PHB) Producer Isolated from the North of Mexico

Alkaline-Tolerant Bacillus cereus 12GS: A Promising Polyhydroxybutyrate (PHB) Producer Isolated from the North of Mexico

Statistical Design-Driven Optimization of Polyhydroxy-Butyrate (PHB) Production Using Sugarcane Bagasse by Brevibacterium sp. (PP989436) Strain

Producing and Characterizing Polyhydroxyalkanoates from Starch and Chickpea Waste Using Mixed Microbial Cultures in Solid-State Fermentation

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