Screening and structural characterization of α-glucosidase inhibitors from hawthorn leaf flavonoids extract by ultrafiltration LC-DAD-MSn and SORI-CID FTICR MS

Li, Huilin; Song, Fengrui; Xing, Junpeng; Tsao, Rong; Liu, Zhiqiang; Liu, Shuying

doi:10.1016/j.jasms.2009.04.003

Cited by 206 publications

(111 citation statements)

References 24 publications

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“…[17] Among MS-based methods, ultrafiltration LC-MS has proved to be a powerful tool for screening biologically active compounds from botanical extracts because of its high-throughput on-line screening ability, and its high sensitivity and selectivity needed for the characterisation of compounds present at low concentrations in highly complex matrices without time-consuming purification procedures. [18] The assays that can be directly performed after TLC separation (bioautography) can be applied to many extracts or fractions at the same time. They are simple and have been widely used for a rapid localisation of bioactive compounds in extracts.…”

Section: Profiling Phytonutrients In Nutraceuticalsmentioning

confidence: 99%

Advanced Methods for Natural Product Drug Discovery in the Field of Nutraceuticals

Wolfender

Eugster

Bohni

et al. 2011

Chimia

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Advances in analytical methods and bioassay development have helped to push forward the research in natural products. In plant extracts and nutraceuticals, bioactive compounds are part of a complex mixture. The development of high-resolution methods related to HPLC for both chemical and biological profiling has significantly increased the efficiency of classical bioactivity-guided fractionation procedures. Furthermore, the level of sensitivity obtained by these methods give the possibility to work with few micrograms of compound. This represents a key advantage for rapid localisation of the biological activity and subsequent identification of the compounds of interest. The same methods are also used to study the extracts from a metabolomic view point. The possibility to study them as a whole can highlight synergetic effects, which are likely to occur in plant extracts and nutraceuticals. In this paper, the main trends are summarised and the developments made in our laboratory on profiling crude extracts with UHPLC-TOF-MS, natural product identification at the microgram level using microflow NMR and integration of these methods with biological evaluation are highlighted.

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Section: Profiling Phytonutrients In Nutraceuticalsmentioning

confidence: 99%

Advanced Methods for Natural Product Drug Discovery in the Field of Nutraceuticals

Wolfender

Eugster

Bohni

et al. 2011

Chimia

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“…11 There is no need to immobilize or modify enzymes before the usage, and the use of free enzymes guaranteed its high sensitivity and selectivity. 12 This method also exhibited advantages like simple operation steps, low sample consumption and simultaneous screening ability. 9 In human body, alpha-amylase could hydrolyze starch into oligosaccharides and become one of the therapeutic targets for treating many diseases, 13 especially diabetes, because they could effectively block the digestion and assimilation of carbohydrate at the early digestive absorption in body.…”

Section: Introductionmentioning

confidence: 99%

Screening and Binding Analysis of Flavonoids with Alpha-Amylase Inhibitory Activity from Lotus Leaf

Liao¹,

Chen²,

Liu³

et al. 2017

J. Braz. Chem. Soc.

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Lotus leaf is gaining growing popularity due to its various benefits and widely usage. In this paper, ten flavonoids in lotus leaf extract were analyzed by high performance liquid chromatography (HPLC). Centrifugal ultrafiltration combined liquid chromatography was used to screen alphaamylase inhibitors from ten flavonoids mixture and the binding degrees ranged from 2.34 to 94.1%. The alpha-amylase inhibition and the 1,1-diphenyl-2-picrylhydrazyl (DPPH) antioxidant activity of ten flavonoids were verified as well. Apigenin, kaempferol and isorhamnetin with relatively higher binding degrees showed higher inhibition on alpha-amylase as well. The interactions between these flavonoids and alpha-amylase were investigated by spectroscopic method. As a result, the fluorescence quenching could be considered as static quenching because the quenching rate constant (K q ) values were higher than 2.0 × 10 10 L mol -1 s -1. The binding constants (log 10 K a ) of ten flavonoids were in the range of 5.971 to 7.417 L mol -1 . The hydrogenation of C 2 =C 3 double bond of apigenin and quercetin decreased the affinity for alpha-amylase (1.92-and 2.82-fold). The hydroxylation on 3 and 3' position decreased the affinity for alpha-amylase. Moreover, the glycosylation with different sugar moiety improved in varying degrees of affinity. The hydrogen bond force might be important in the binding between alpha-amylase and flavonoids.

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“…11 The ability of vitexin to inhibit adipogenesis in 3T3 L1 cells has also been demonstrated. 12 Both vitexin and isovitexin possess strong αglucosidase inhibitory activity 13 as well as antiglycation abil ity.…”

Section: Introductionmentioning

confidence: 99%

Comprehensive Assessment of Antioxidant Activities of Apigenin Isomers: Vitexin and Isovitexin

Khole

Panat

Suryawanshi

et al. 2016

FRA

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Introduction: Fenugreek is known to possess anti-diabetic as well as antioxidant properties known to increase with germination. Previous study from our laboratory has reported vitexin and isovitexin as major antioxidant compounds from germinated fenugreek seeds. The present study aimed to comprehensively assesses and compare their antioxidant activity. Methods: The antioxidant ability of these compounds against various radicals was checked using standard in vitro radical scavenging assays and pulse radiolysis. Protection conferred to mitochondria against oxidative damage was assessed by measuring the levels of lipid peroxides and protein sulphydryls. Protection from hydrogen peroxide induced cytotoxicity in HepG2 cells was checked by MTT assay and by measuring intracellular ROS. Their ability to modulate intracellular antioxidant status was checked both at activity and RNA level. Result: Vitexin and isovitexin exhibited differential antioxidant activity against various radicals. Vitexin was a better nitric oxide scavenger while isovitexin scavenged superoxide radicals more efficiently. Pulse radiolysis studies revealed good antioxidant activity of both compounds against short-lived radicals and affected the formation and decay of the 2-dG transient species to the same extent. In oxidatively damaged mitochondria, lipid peroxidation was inhibited significantly by isovitexin whereas vitexin prevented decrease in protein sulphydryl content more effectively. These compounds protected HepG2 cells to the same extent against hydrogen peroxide induced oxidative insult by reducing intracellular ROS and modulating the levels of antioxidant enzymes. Conclusion: The present study clearly demonstrates differential antioxidant potential of both vitexin and isovitexin and their role in mitigating oxidative stress induced damage and maintaining cellular redox homeostasis.

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Screening and structural characterization of α-glucosidase inhibitors from hawthorn leaf flavonoids extract by ultrafiltration LC-DAD-MSⁿ and SORI-CID FTICR MS

Cited by 206 publications

References 24 publications

Advanced Methods for Natural Product Drug Discovery in the Field of Nutraceuticals

Advanced Methods for Natural Product Drug Discovery in the Field of Nutraceuticals

Screening and Binding Analysis of Flavonoids with Alpha-Amylase Inhibitory Activity from Lotus Leaf

Comprehensive Assessment of Antioxidant Activities of Apigenin Isomers: Vitexin and Isovitexin

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