SCREENING AND STRUCTURAL CHARACTERIZATION OF MMP-2 INHIBITOR FROM CARTHAMUS TINCTORIUS L. USING ULTRAFILTRATION LIQUID CHROMATOGRAPHY–MASS SPECTROMETRY

UF-LC-DAD-MS n for discovering enzyme inhibitors for nutraceuticals and functional foods

“…Finding selective inhibitors of MMP-2, 5-LOX or COX-2 from herb extracts has become increasingly a hot research topic in recent years. UF-LC-MS n has been proven useful for high-throughput screening and identification of MMP-2, 5-LOX or COX-2 inhibitors from some plant extracts (Li et al, 2014;Li et al, 2015;.…”

“…Similar ultrafiltration LC-MS screening has been conducted for MMP-2 inhibitors from C. tinctorius, S. glabra, Smilax china L. and S. divaricate (Li et al, 2014;Li et al, 2015). S. glabra and S. china are widely consumed by Chinese as functional/medicinal foods for improving immunity and general health.…”

“…Membrane ultrafil-tration with different molecular cut-offs has been used for isolation and purification of macromolecules (He et al, 2013). However in the last few years, this technology has been coupled with liquid chromatography-diode array detector-mass spectrometry (LC-DAD-MS) for high-throughput screening and discovery of enzyme inhibitors from herb extracts and plant foods (Cao et al, 2010;Choi et al, 2011;Li et al, 2014;Li et al, 2015;Li et al, 2009;Liu et al, 2012a, b;Shi et al, 2013;van Breemen et al, 2011;Yang et al, 2012;Zhang et al, 2012;Shi et al, 2010;Zhou et al, 2012 ). In ultrafiltration LC-DAD-MS (UF-LC-MS) experiments, when a mixture of compounds (ligands) is in contact with the target enzyme (a macromolecular receptor), the ultrafiltration membrane retains the macromolecules and the ligandreceptor complexes, but allows unbound, low molecular weight compounds to pass through.…”