Screening Antibacterial Agent from Crude Extract of Marine-Derived Fungi Associated with Soft Corals against MDR-<i>Staphylococcus haemolyticus</i>

Sabdaningsih, Aninditia; Cristianawati, Olvi; Sibero, Mada Triandala; Nuryadi, Handung; Radjasa, O K; Sabdono, Agus; Trianto, Agus

doi:10.1088/1755-1315/55/1/012026

Cited by 12 publications

(13 citation statements)

References 21 publications

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“…The fungal species showed high cytotoxic activity against human liver carcinoma cell line (HEPG-2) with IC 50 value 18.8 µg/ml. In close relation, the culture filtrate extract of A. tritici isolated from the coral Galaxea fascicularis collected at Port Dickson, Malaysia, showed cytotoxic and antimicrobial activities (Sabdaningsih et al 2016 showed potent cytotoxicity against human lung cancer cell line A549, human nasopharyngeal carcinoma cell line CNE2 and human colon cancer cell line Lovo (Li et al 2012). The LC-MS fingerprints of the A. ochraceopetaliformis EtOAc extract…”

Section: Cytotoxicity Of Coral Reef Symbiotic Fungi Against Tumour Cementioning

confidence: 93%

Biological activities and variation of symbiotic fungi isolated from Coral reefs collected from Red Sea in Egypt

et al. 2020

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Ten specimens of coral reefs were collected from the Red Sea in the Ein El-Sukhna region. Fungal isolation was done using two media, Dextrose Yeast Extract Agar (DYA) and Rose Bengal Agar (RBA). The morphological traits identified 18 fungal isolates belonging to the phyla Ascomycota, Mucoromycota and Deuteromycota. Five genera in three orders have been isolated: Eutrotiales (Aspergillus, Penicillium and Byssochlamys), Mucorales (Rhizopus) and Moniliales (Curvularia). The heat mapping clustering of the isolated fungi declared that Aspergillus and Penicillium were the most frequently isolate fungi in coral reefs. It was found that A. fumigatus colonised eight coral samples with 80% colonisation rate. Moreover, about 50% of the isolated fungal species were specific to one coral reef only such as A.candidus and A.carneus isolated from Isophyllastrea rigida only, A.japonicus and A.ochraceopetaliformis from Glaxaea fascicularis, A.niger van Tieghem from Porites astreoides, A.sydowii, A.terreus and P.waksmanii from Cladocora arbuscula, P.janthinellum from Pterogorgia guadalupensis and Curvularia tuberculata, Byssochlamys spectabilis and Rhizopus oryzae from Acropora humilis. Biological activities (antimicrobial, antioxidant antiradical and cytotoxicity) of the most predominant fungal species were investigated. The antimicrobial activity of coral fungal filtrates were investigated against six pathogenic bacteria including Escherichia coli ATCC11775, Neisseria gonorrhoeae ATCC19424, Pseudomonas aeruginosa ATCC10145, Streptococcus faecalis ATCC19433, Staphylococcus aureus subsp. aureus ATCC25923, Bacillus subtilis subsp. spizizenii ATCC6633 and two pathogenic yeast including Candida albicans ATCC7102 and Candida parapsilosis ATCC22019. Most of these fungal filtrates exhibited moderate to high antibacterial activities against both gram positive and gram negative bacteria, however it showed relatively low bioactivity towards the pathogenic Candida species. Investigating the free radical scavenging activity using DPPH reagent showed low to moderate bioactivities. The highest cytotoxic activity against liver cancer cell line Hep-G2 with an IC 50 values of 18.8 µg/ml was exhibited by Aspergillus ochraceopetaliformis MN083316 and a metabolomics study was done on the ethyl acetate extract of this strain using LC-ESI-MS fingerprints leading to the isolation and purification of compound 1. Using 1D and 2D NMR techniques compound 1 was identified as ditryptophenaline. Compound 1 exhibited a strong antimicrobial, antioxidant activities as well as cytotoxic activities against MCF-7 and HEPG2 with IC 50 values of 5.8 and 7.6 mmole, respectively.The objective of this study, isolation of Coral-reef associated fungi and studying their biological activities to produce the most active secondary metabolite which might possess a novel biological activity. ARTICLE HISTORY

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Section: Cytotoxicity Of Coral Reef Symbiotic Fungi Against Tumour Cementioning

confidence: 93%

Biological activities and variation of symbiotic fungi isolated from Coral reefs collected from Red Sea in Egypt

et al. 2020

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“…This is strongly indicating that sponge-associated fungi from Indonesia are still underevaluated. Therefore, exploration of novel compounds from fungi associating with Indonesian sponges is currently a major subject in our research group [16][17][18].…”

Section: Introductionmentioning

confidence: 99%

Two new aromatic polyketides from a sponge-derived Fusarium

Sibero¹,

Zhou²,

Fukaya³

et al. 2019

Beilstein J. Org. Chem.

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In our natural product screening program from marine fungi, two new aromatic polyketides karimunones A (1) and B (2) and five known compounds (3–7) were isolated from sponge-associated Fusarium sp. KJMT.FP.4.3 which was collected from an Indonesian sponge Xestospongia sp. The structures of these compounds were determined by the analysis of NMR and MS spectroscopic data. The NMR assignment of 1 was assisted by DFT-based theoretical chemical shift calculation. Compound 2 showed antibacterial activity against multidrug resistant Salmonella enterica ser. Typhi with a MIC of 125 µg/mL while 1 was not active.

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“…The agar plates were incubated at room temperature (25 • C) for three days. The initial selection of fungal colonies was done based on phenotype, e.g., colony morphology and color [40]. In total, 20 fungal colonies were isolated and propagated until axenic cultures were obtained from ten sponges.…”

Section: Fungal Isolation and Purificationmentioning

confidence: 99%

A New Citrinin Derivative from the Indonesian Marine Sponge-Associated Fungus Penicillium citrinum

et al. 2020

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Sponge-associated fungi are attractive targets for the isolation of bioactive natural products with different pharmaceutical purposes. In this investigation, 20 fungi were isolated from 10 different sponge specimens. One isolate, the fungus Penicillium citrinum strain WK-P9, showed activity against Bacillus subtilis JH642 when cultivated in malt extract medium. One new and three known citrinin derivatives were isolated from the extract of this fungus. The structures were elucidated by 1D and 2D NMR spectroscopy, as well as LC-HRMS. Their antibacterial activity against a set of common human pathogenic bacteria and fungi was tested. Compound 2 showed moderate activity against Mycobacterium smegmatis ATCC607 with a minimum inhibitory concentration (MIC) of 32 µg/mL. Compound 4 exhibited moderate growth inhibition against Bacillus subtilis JH642, B. megaterium DSM32, and M. smegmatis ATCC607 with MICs of 16, 16, and 32 µg/mL, respectively. Furthermore, weak activities of 64 µg/mL against B. subtilis DSM10 and S. aureus ATCC25923 were observed for compound 4.

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Screening Antibacterial Agent from Crude Extract of Marine-Derived Fungi Associated with Soft Corals against MDR-Staphylococcus haemolyticus

Cited by 12 publications

References 21 publications

Biological activities and variation of symbiotic fungi isolated from Coral reefs collected from Red Sea in Egypt

Biological activities and variation of symbiotic fungi isolated from Coral reefs collected from Red Sea in Egypt

Two new aromatic polyketides from a sponge-derived Fusarium

A New Citrinin Derivative from the Indonesian Marine Sponge-Associated Fungus Penicillium citrinum

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