Screening Antitumor Bioactive Fraction from Sauromatum giganteum (Engl.) Cusimano &amp; Hett and Sensitive Cell Lines  with the Serum Pharmacology Method and Identification  by UPLC-TOF-MS

Gao, Shuangquan; Gong, Yun-Fei; Sun, Qiu-Jia; Bai, Jing; Wang, Long; Fan, Ziquan; Sun, Yi-Ming; Su, Yijun; Gang, Jian; Yu-bin, JI

doi:10.3390/molecules20034290

Cited by 14 publications

(6 citation statements)

References 34 publications

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“…MMP reduction is also involved in oxidative stress, and may result in reactive oxygen species (ROS) release from mitochondria. 27,28 ROS generation in NCI-H460 cells ROS is dynamically equilibrated between antioxidant defense mechanisms and cytotoxic responses leading to cell apoptosis. [29][30][31] In particular, ROS generation could trigger a wide range of transcriptional changes as the second messengers in a signaling cascade, and it might be recovered after leading to apoptosis or cell death.…”

Section: Effects On the Mmpmentioning

confidence: 99%

Correlation of the cytotoxic effects of cationic lipids with their headgroups

et al. 2018

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As effective non-viral vectors of gene therapy, cationic lipids still have the problem of toxicity, which has become one of the main bottlenecks for their applications. The toxicity of cationic lipids is strongly connected to the headgroup structures. In this article, we studied the cytotoxicity of two cationic lipids with a quaternary ammonium headgroup (CDA14) and a tri-peptide headgroup (CDO14), respectively, and with the same linker bond and hydrophobic domain. The IC values of CDA14 and CDO14 against NCI-H460 cells were 109.4 μg mL and 340.5 μg mL, respectively. To determine the effects of headgroup structures of cationic lipids on cytotoxicity, apoptosis related pathways were investigated. As the lipids with a quaternary ammonium headgroup could induce more apoptotic cells than the ones with a peptide headgroup, the enzymatic activity of caspase-9 and caspase-3 increased obviously, whereas the mitochondrial membrane potential (MMP) decreased. At the same time, the reactive oxygen species (ROS) levels also increased and the cell cycle was arrested at the S phase. The results showed that the toxicity of the cationic lipid had a close relationship with its headgroup structures, and the cytotoxic mechanism was mainly the caspase activation dependent signaling pathway and mitochondrial dysfunction. Through this study, we hope to provide the scientific basis for exploiting safer and more efficient cationic lipids for gene delivery.

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Section: Effects On the Mmpmentioning

confidence: 99%

Correlation of the cytotoxic effects of cationic lipids with their headgroups

et al. 2018

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“…Serum pharmacology can objectively simulate the interactions between drugs and the body, and the pharmaceutical ingredients contained in serum are also the endogenous active ingredients produced by the body under the reactions of active ingredients and drugs via a series of in vivo biotransformations. In addition, the physical and chemical properties of serum are similar to that of the internal environments in which the cells are present (14,15). Therefore, if TFA directly acts on cells in vitro, the in vivo metabolic process will remain unclear, thus it cannot truly reflect the pharmacological effects of TFA in vivo.…”

Section: Discussionmentioning

confidence: 99%

Impacts of the serum containing total flavonoids of Ajuga on rat glomerular mesangial cells

Nan

Huang

Lai

et al. 2017

Molecular Medicine Reports

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The aim of the current study was to investigate the impacts and possible mechanisms of total flavonoids of Ajuga (TFA) on glomerular mesangial cells (GMC) through in vitro observations of the impacts of TFA‑containing serum on GMC proliferation and extracellular matrix (ECM) secretion in lipopolysaccharides (LPS)‑induced rats. Rat GMC was cultured in vitro, using LPS to stimulate the proliferation of GMC and the secretion of ECM; meanwhile, TFA‑containing serum (TFA‑S) was used for the intervention. Methyl thiazolyl tetrazolium (MTT) assay was performed to test the proliferation of GMC; enzyme‑linked immunosorbent assay (ELISA) was used to detect the expressions of fibronectin (FN) and collagen IV (Col‑IV) in cell supernatant, flow cytometry was performed to detect the cell cycle, and reverse transcription-polymerase chain reaction was performed to detect the expression levels of matrix metalloproteinase 9 (MMP‑9) mRNA and transforming growth factor β1 (TGF‑β1) mRNA. The GMC proliferation and the expressions of FN and Col‑IV in cell supernatant were significantly reduced after 24 and 48 h TFA‑S intervention (P<0.05 or 0.01). A total of 48 h subsequent to the intervention, the proportion of GMC in the G1 phase and the relative expression of MMP‑9 mRNA were significantly increased (P<0.05 or 0.01), however the proportion of GMC in S phase and the relative expression of TGF‑β1 mRNA were significantly reduced (P<0.05 or 0.01). TFA‑S can inhibit LPS‑induced GMC proliferation and ECM accumulation, and its roles are associated with regulating the cell cycle and the expression levels of TGF‑β1 and MMP‑9.

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“…Each dry powder (200 g) of TCMs was extracted with 1000 mL cyclohexane (CYH) firstly, and then the leftover solution was sequentially extracted using dichloromethane (DCM), ethyl acetate (EAC), n -butanol (NBA), and distilled water (W), according to the method described by Liu et al [ 13 , 14 ]. The five extracted solutions of 100 TCMs were harvested separately by ultrasonic extraction at 40°C for 30 min and repeated twice followed by filtration and evaporation under vacuum.…”

Section: Methodsmentioning

confidence: 99%

Screening for Anti‐Influenza Actives of Prefractionated Traditional Chinese Medicines

Kang

Wang

Cui

et al. 2020

Evidence-Based Complementary and Alternative Medicine

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Traditional Chinese medicines (TCMs) have proven to possess advantages in counteracting virus infections according to clinical practices. It’s therefore of great value to discover novel antivirals from TCMs. In this paper, One hundred medicinal plants which have been included in TCM prescriptions for antiviral treatment were selected and prefractionated into 5 fractions each by sequentially using cyclohexane, dichloromethane, ethyl acetate, n-butanol, and water. 500 TCM-simplified extracts were then subjected to a phenotypic screening using a recombinant IAV expressing Gaussia luciferase. Ten TCM fractions were identified to possess antiviral activities against influenza virus. The IC50’s of the hit fractions range from 1.08 to 6.45 μg/mL, while the SIs, from 7.52 to 98.40. Furthermore, all the ten hit fractions inhibited the propagation of progeny influenza virus significantly at 20 μg/mL. The hit TCM fractions deserve further isolation for responsible constituents leading towards anti-influenza drugs. Moreover, a library consisting of 500 simplified TCM extracts was established, facilitating antiviral screening in quick response to emerging and re-emerging viruses such as Ebola virus and current SARS-CoV-2 pandemic.

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Screening Antitumor Bioactive Fraction from Sauromatum giganteum (Engl.) Cusimano & Hett and Sensitive Cell Lines with the Serum Pharmacology Method and Identification by UPLC-TOF-MS

Cited by 14 publications

References 34 publications

Correlation of the cytotoxic effects of cationic lipids with their headgroups

Correlation of the cytotoxic effects of cationic lipids with their headgroups

Impacts of the serum containing total flavonoids of Ajuga on rat glomerular mesangial cells

Screening for Anti‐Influenza Actives of Prefractionated Traditional Chinese Medicines

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