Screening approaches and therapeutic targets: The two driving wheels of tuberculosis drug discovery

Perveen, Summaya

doi:10.1016/j.bcp.2021.114906

Cited by 19 publications

(14 citation statements)

References 174 publications

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“…The macrophages engulf the microbes by a process called phagocytosis resulting in the formation of the phagosome [ 6 ]. The interaction between the mycobacterial conserved molecular patterns with the pathogen recognition receptors like Toll-like receptors (TLRs), nucleotide-binding oligomerization domain-like receptors and C -type lectins on macrophages, causes stimulation of the innate immune response against Mtb like phagocytosis and activation proinflammatory cytokines production [ 7 , 8 ].…”

Section: Pathogenesismentioning

confidence: 99%

Evolution of tuberculosis diagnostics: From molecular strategies to nanodiagnostics

2023

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Section: Pathogenesismentioning

confidence: 99%

Evolution of tuberculosis diagnostics: From molecular strategies to nanodiagnostics

2023

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“…Acetyl-CoA is then converted to citrate, isocitrate, and carbon dioxide in the tricarboxylic acid (TCA) cycle. The glyoxylate shunt converts isocitrate into glyoxylate and succinate (ICL I and ICL II), aiding carbon conservation . Substrate-level glycolysis produces CO 2 and reduces NAD + to NADH.…”

Section: Targeting Energy Metabolismmentioning

confidence: 99%

Revolutionizing Tuberculosis Treatment: Uncovering New Drugs and Breakthrough Inhibitors to Combat Drug-Resistant Mycobacterium tuberculosis

Verma,

Naik,

Kumar

et al. 2023

ACS Infect. Dis.

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Tuberculosis (TB) is a global health threat that causes significant mortality. This review explores chemotherapeutics that target essential processes in Mycobacterium tuberculosis, such as DNA replication, protein synthesis, cell wall formation, energy metabolism, and proteolysis. We emphasize the need for new drugs to treat drug-resistant strains and shorten the treatment duration. Emerging targets and promising inhibitors were identified by examining the intricate biology of TB. This review provides an overview of recent developments in the search for anti-TB drugs with a focus on newly validated targets and inhibitors. We aimed to contribute to efforts to combat TB and improve therapeutic outcomes.

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“…Among the rapid Mtb nucleic acid diagnostic tests, WHO recommends using Xpert MTB/RIF Ultra and TB LAMP. Together with other technologies endorsed by WHO, these tests proved effective in diagnosing HIV-associated and different resistant forms of TB [92][93][94][95][96].…”

Section: Tb Tb Ismentioning

confidence: 99%

CRISPR-Based Programmable Nucleic Acid-Binding Protein Technology Can Specifically Detect Fatal Tropical Disease-Causing Pathogens

Rahman¹,

Majumder²,

Apu

et al. 2022

Journal of Tropical Medicine

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Diagnostic approaches capable of ultrasensitive pathogen detection from low-volume clinical samples, running without any sophisticated instrument and laboratory setup, are easily field-deployable, inexpensive, and rapid, and are considered ideal for monitoring disease progression and surveillance. However, standard pathogen detection methods, including culture and microscopic observation, antibody-based serologic tests, and primarily polymerase chain reaction (PCR)-oriented nucleic acid screening techniques, have shortcomings that limit their widespread use in responding to outbreaks and regular diagnosis, especially in remote resource-limited settings (RLSs). Recently, clustered regularly interspaced short palindromic repeats (CRISPR)-based programmable technology has emerged to challenge the unmet criteria of conventional methods. It consists of CRISPR-associated proteins (Cas) capable of targeting virtually any specific RNA or DNA genome based on the guide RNA (gRNA) sequence. Furthermore, the discovery of programmable trans-cleavage Cas proteins like Cas12a and Cas13 that can collaterally damage reporter-containing single-stranded DNA or RNA upon formation of target Cas-gRNA complex has strengthened this technology with enhanced sensitivity. Current advances, including automated multiplexing, ultrasensitive single nucleotide polymorphism (SNP)-based screening, inexpensive paper-based lateral flow readouts, and ease of use in remote global settings, have attracted the scientific community to introduce this technology in nucleic-acid-based precise detection of bacterial and viral pathogens at the point of care (POC). This review highlights CRISPR-Cas-based molecular technologies in diagnosing several tropical diseases, namely malaria, zika, chikungunya, human immunodeficiency virus and acquired immunodeficiency syndrome (HIV-AIDS), tuberculosis (TB), and rabies.

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Screening approaches and therapeutic targets: The two driving wheels of tuberculosis drug discovery

Cited by 19 publications

References 174 publications

Evolution of tuberculosis diagnostics: From molecular strategies to nanodiagnostics

Evolution of tuberculosis diagnostics: From molecular strategies to nanodiagnostics

Revolutionizing Tuberculosis Treatment: Uncovering New Drugs and Breakthrough Inhibitors to Combat Drug-Resistant Mycobacterium tuberculosis

CRISPR-Based Programmable Nucleic Acid-Binding Protein Technology Can Specifically Detect Fatal Tropical Disease-Causing Pathogens

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