Screening, expression, and characterization of an anti-human oxidized low-density lipoprotein single-chain variable fragment

Kumano-Kuramochi, Miyuki; Fujimura, Takashi; Komba, Shiro; Maeda‐Yamamoto, Mari; Machida, Sachiko

doi:10.1016/j.jbiosc.2016.02.014

Cited by 5 publications

(5 citation statements)

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“…Some reports indicated that the statins that inhibit HMG-CoA reductase gene expression can lower serum cholesterol levels, but taking these drugs for long periods of time and at high doses can lead to hepatocellular damage, rhabdomyolysis, and resistance as well as intolerance (9)(10)(11)42). The findings in this study showed no changes in AST and ALT levels in the serum following higher doses of CZ consumption, indicating no harmful effects on the liver.…”

Section: -mentioning

confidence: 42%

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The Effects of Cinnamomum zeylanicum on Lipid Profiles and Histology Via Up-Regulation of LDL Receptor Gene Expression in Hamsters Fed a High Cholesterol Diet

Kassaee

Goodarzi

Roodbari

et al. 2016

Jundishapur J Nat Pharm Prod

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Background: Recently, medicinal plants have become the focus of more attention due to their potentially effective roles in the prevention and treatment of many diseases. Objectives: The aim of this research was to examine the effects of Cinnamomum zeylanicum (CZ) on lipid profile and histological changes due to LDL receptor (LDL-R) gene expression in hamsters fed a high cholesterol diet. Methods: Thirty-two male hamsters were allocated into four groups (eight in each group under four weeks of treatment) as follows: group ND were fed a standard normal diet; group HCD were fed a normal diet supplemented with high cholesterol; group HCD + CZ2 were fed a high cholesterol diet (HCD) along with 2% CZ; and group HCD + CZ8 were fed an HCD diet along with 8% CZ. After the treatment process, blood samples were taken from all of studied animals, and sera were prepared for lipid profile assays. Liver LDL-R gene expression was assayed using the real-time polymerase chain reaction (PCR) method. Liver histological changes were determined using the hematoxylin and eosin staining method. Results: A significant reduction was observed in serum triglycerides (TG), total cholesterol (TC), low-density lipoprotein cholesterol (LDL-c), very-low-density lipoprotein cholesterol (VLDL-c), aspartate transaminase (AST), and alanine transaminase (ALT) levels in the HCD + CZ8 group, while serum high density lipoprotein cholesterol (HDL-c) levels increased when compared with the HCD group (P < 0.001). The HCD group demonstrated decreased LDL-R gene expression in the animals' livers, whereas treatment with CZ upregulated its expression. The scores for hepatic steatosis in the HCD + CZ8 group were diminished significantly. Conclusions:The administration of CZ improved dyslipidemia in hypercholesterolemic animals, probably by increasing LDL-R gene expression in the liver; consequently, it can be concluded that CZ can reverse hepatic steatosis. Therefore, CZ was determined as having a protective role against hypercholesterolemia.

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Section: -mentioning

confidence: 42%

“…The hypolipidemic effects of some plant extracts may be due to decreases in hepatic-3-hydroxy-3-methylglutarylCoA reductase, cholesterol 7α-hydroxylase, pentosephosphate pathways, or antioxidant activities (3,21,24,41,42). Other mechanisms have also been reported.…”

Section: -mentioning

confidence: 99%

The Effects of Cinnamomum zeylanicum on Lipid Profiles and Histology Via Up-Regulation of LDL Receptor Gene Expression in Hamsters Fed a High Cholesterol Diet

Kassaee

Goodarzi

Roodbari

et al. 2016

Jundishapur J Nat Pharm Prod

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“…OxLDL-Ab level was the best indicator for both hospitalization and composite outcome [36]. It is excited that a new approach of determination OXLDL antibody component, a novel single-chain variable fragments (scFvs) γκ5, has been established and the γκ5 scFv can detect the modified LDL at very low concentrations [37]. This will promote the deep study of the relationship between oxLDL antibodies and diseases.…”

Section: Discussionmentioning

confidence: 99%

The association between oxidized low-density lipoprotein antibodies and hematological diseases

et al. 2016

Lipids Health Dis

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BackgroundThe aim of the study is to compare the profiles of antibodies (IgM and IgG) against oxidized low-density lipoprotein (oxLDL) of hematological diseases.MethodsThe serum antibodies of oxLDL-IgM and oxLDL-IgG for 446 cases with hematological diseases and 90 patients with primary hypertension and 90 healthy controls were measured by enzyme-linked immunosorbent assay (ELISA) in a cross-section survey. The association of serum oxLDL-LgM and oxLDL-IgG with hematological diseases was analyzed by multiple linear regression model.ResultsComparing with the hypertension or normal groups, the levels of TCH, TG, LDL-c, HDL-c, oxLDL, and oxLDL-IgG were lower and the levels of ADP and oxLDL-IgM were higher in the hematological diseases group. The levels of oxLDL-IgG antibodies titer were different among hematological diseases group. The results of correlation and multiple regression analysis showed that the seven hematological disease subgroups were positively related to the oxLDL-IgM antibody titer but negatively related to the oxLDL-IgG antibody titer, having been adjusted for potential confounding factors such as age, SBP, DBP, BMI, TCH, TG, ADP, oxLDL, HDL-c, LDL-C.ConclusionsHere we show that oxLDL-IgG antibodies titer were lower and of oxLDL-IgM titer were higher than hypertension and healthy individuals. Also oxLDL-IgG titer were different among hematological diseases group.

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“…Platform IV (new functionality analysis), headed by Gen-Ichiro Soma (Control of Innate Immunity Technology Research Association), investigates bodily homeostasis for the assessment of food functionalities. A number of papers and patents have been produced [81][82][83][84][85][86][87][88][89][90][91][92][93][94][95]. To evaluate various aspects of the effect of AFF food products and sports on the bodily homeostasis, in addition to the usual biochemical testing, a basic system was constructed that enables using trace amounts (μl) of untreated blood samples for neutrophil, oxidized LDL, and phagocyte analyses.…”

Section: S I P: Cross-ministerial Strategic Innovation Promotion Progmentioning

confidence: 99%

“…The research group involved in this platform has also proposed an integrated protocol for the use of blood samples of animal and human origin. The most important accomplishments of the group thus far include (1) the first-ever patent pertaining to a construction of a device that can be used for simultaneous measurement of superoxide and myeloperoxidase activities [92,95]; (2) detection of oxidized LDL in human plasma using oxidized LDL receptor that is engineered in transgenic silkworms (detection limit: 10 ng/ml) [82,87,91,93]; and (3) construction of a firstever double-tube device for measuring phagocytosis [83,90,94] in the human peripheral blood. The members of the group are collaborating with platform I, II, and III group members to detect correlations between conventional biochemical parameters and these homeostasis monitoring factors ( Figure 5).…”

Section: S I P: Cross-ministerial Strategic Innovation Promotion Progmentioning

confidence: 99%

Food functionality research as a new national project in special reference to improvement of cognitive and locomotive abilities

Abe

Misaka

2018

Bioscience, Biotechnology, and Biochemistry

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In Japan, where a super-aging society is realized, we are most concerned about healthy longevity, which would ascertain the wellness of people by improving their quality of life (QOL). In 2014, the Cabinet Office proposed a strategic innovation promotion programme, launching a national project for the development of the agricultural-forestry-fisheries food products with new functionalities for the next generation. In addition to focusing on a conventional prevention of lifestyle-associated metabolic syndromes, the project targets the scientific evidence of the activation of brain cognitive ability and the improvement of bodily locomotive function. The project also involves the analysis of the foods-sports interrelation of chronic importance, and the development of devices for the verification of QOL-associated maintenance of homeostasis. In this review, we provide an overview of these studies, with special reference to cognition as a case of the gut-brain axis which the author is particularly interested in.

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Screening, expression, and characterization of an anti-human oxidized low-density lipoprotein single-chain variable fragment

Cited by 5 publications

References 32 publications

The Effects of Cinnamomum zeylanicum on Lipid Profiles and Histology Via Up-Regulation of LDL Receptor Gene Expression in Hamsters Fed a High Cholesterol Diet

The Effects of Cinnamomum zeylanicum on Lipid Profiles and Histology Via Up-Regulation of LDL Receptor Gene Expression in Hamsters Fed a High Cholesterol Diet

The association between oxidized low-density lipoprotein antibodies and hematological diseases

Food functionality research as a new national project in special reference to improvement of cognitive and locomotive abilities

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