Screening for anti-inflammatory components from Corydalis bungeana Turcz. based on macrophage binding combined with HPLC

Abstract

BackgroundCorydalis bungeana Turcz. (CB; family: Corydalis DC.) is an anti-inflammatory medicinal herb used widely in traditional Chinese medicine (TCM) for upper respiratory tract infection, etc., but its anti-inflammatory active molecules are unknown. This study was designed to screen for the anti-inflammatory components from CB based on macrophage binding combined with HPLC.MethodsXylene-induced ear edema in mouse and carrageenan-induced hind-paw edema in rats were used to evaluate the anti-inflammatory activity of CB. The macrophage binding with high-performance liquid chromatography (HPLC) analysis and HPLC-MS were established to screen the potential active compounds. ELISA kits were performed to measure the levels of IL-6, IL-10, TNF-α and NO in RAW 264.7 macrophages culture media.ResultsThe alkaloid extract of CB could inhibit significantly xylene-induced ear edema in mouse and carrageenan-induced hind-paw edema in rats. Two components binded to RAW 264.7 cell were identified as 12-hydroxycorynoline and corynoline. Bioassays demonstrated that these two compounds significantly inhibited LPS-induced IL-6, IL-10, TNF-α and NO levels.ConclusionsThe results suggest that corynoline and 12-hydroxycorynoline contribute to the anti-inflammatory effects of the alkaloid extract of CB. Our findings suggest that these two compounds can be used as candidate for anti-inflammatory drugs.

Biologically active isoquinoline alkaloids covering 2014–2018

“…Corynoline (314) from Corydalis bungeana possesses anti-inflammatory and antibacterial activities. 96 Ambinine 315 317 also was found in the whole plant of C. pallida. 73 8-Methoxydihydrosanguinarine (319) and dihydrosanguinarine (320) were obtained from C. mucronifera 82 ( Figure 15).…”

“…56 In 2017, N-formyl-asimilobine-2-O-β-D-glucoside (95), an amidic aporphine, was obtained from the tubers of Stephania succifera. 57 Four aporphine alkaloids, isoboldine (96), anonaine (97), nornuciferine (98), and actinodaphnine (99), were isolated from Annona hypoglauca. 58 Magnoflorine (100) was found in the rhizomes of M.…”

“…31 Corynoline (314) from C. bungeana inhibited inflammatory mediators in LPS-stimulated RAW264.7 cells and attenuated LPS-induced acute lung injury in mice by activating Nrf2. 96,292,293 The unique alkaloid dactyllactone A (126) inhibited the expression of IL-1β and PGE2 in a dose-dependent manner in LPS-induced RAW264.7 cells. 64 Decarine 313…”

Metabolism‐based herb–drug interaction of Corydalis Bungeanae Herba with berberine in vitro and in vivo in rats

“…Corydalis Bungeanae Herba ( C. bungeanae ), known as Kudiding in Chinese, is widely used in Asian nations (Xie, Veitch, Houghton, & Simmonds, ). The dried whole plant is used for clearing heat and toxins, as well as anti‐inflammatory effects for the treatment of various infectious diseases (Dong et al, ; Niu et al, ), and it has been officially recorded in the Chinese Pharmacopoeia (2015 version). Isoquinoline alkaloid corynoline (CRL) (Scheme ) was found to be the main functional component in the herb (Yang et al, ), with potent immunosuppressive activity and inhibitory effects on inflammation (Kamigauchi et al, ).…”

Metabolism and Bioactivation of Corynoline With Characterization of the Glutathione/Cysteine Conjugate and Evaluation of Its Hepatotoxicity in Mice

“…CRL is the only ingredient for assessing the quality of this herbal medicine. Furthermore, it has been proved that CRL have many pharmacology activities, including anti-inflammation ( Dong et al, 2015 ; Zhai et al, 2016 ), cell adhesion inhibitory ( Kamigauchi et al, 2005 ), inhibition of acetylcholinesterase ( Kim, 2002 ) and β-site amyloid precursor protein cleaving enzyme 1 (BACE1) ( Chlebek et al, 2016 ), cytotoxic activities ( Choi et al, 2007 ) and so on, which have captured the interest of scientists in recent years.…”